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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
14/10/2016 |
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Data da última atualização: |
03/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, F. R. M.; DEUS, K. E. de; PEREIRA, W. J.; SILVEIRA, R. D. D.; VIANELLO, R. P.; BRONDANI, C. |
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Afiliação: |
FERNANDA RAQUEL MARTINS ABREU, UNIVERSIDADE FEDERAL DE GOIÁS; KARINE EVARISTO DE DEUS, UNIVERSIDADE FEDERAL DE GOIÁS; WENDELL JACINTO PEREIRA, UNIVERSIDADE FEDERAL DE GOIÁS; RICARDO DIOGENES DIAS SILVEIRA; ROSANA PEREIRA VIANELLO, CNPAF; CLAUDIO BRONDANI, CNPAF. |
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Título: |
Expression of rice genes homologous of Arabidopsis genes previously related to drought tolerance. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 10, n. 9, p. 1266-1271, 2016. |
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ISSN: |
1835-2707 |
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Idioma: |
Inglês |
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Conteúdo: |
The identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive stages, whereas in Primavera, this gene was suppressed in both tissues and stages under drought. Therefore, the OsPLDa1 gene was the most important in relation to drought response and is an interesting candidate for further studies in developing rice cultivars that are more tolerant to this stress. MenosThe identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive s... Mostrar Tudo |
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Palavras-Chave: |
Gene homology. |
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Thesagro: |
Acido abscisico; Arroz; Oryza sativa; Rendimento. |
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Thesaurus Nal: |
Abscisic acid; Arabidopsis; Quantitative polymerase chain reaction; Rice; Yields. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148788/1/CNPAF-2016-ajcs.pdf
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Marc: |
LEADER 02695naa a2200313 a 4500
001 2054691
005 2017-03-03
008 2016 bl uuuu u00u1 u #d
022 $a1835-2707
100 1 $aABREU, F. R. M.
245 $aExpression of rice genes homologous of Arabidopsis genes previously related to drought tolerance.$h[electronic resource]
260 $c2016
520 $aThe identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive stages, whereas in Primavera, this gene was suppressed in both tissues and stages under drought. Therefore, the OsPLDa1 gene was the most important in relation to drought response and is an interesting candidate for further studies in developing rice cultivars that are more tolerant to this stress.
650 $aAbscisic acid
650 $aArabidopsis
650 $aQuantitative polymerase chain reaction
650 $aRice
650 $aYields
650 $aAcido abscisico
650 $aArroz
650 $aOryza sativa
650 $aRendimento
653 $aGene homology
700 1 $aDEUS, K. E. de
700 1 $aPEREIRA, W. J.
700 1 $aSILVEIRA, R. D. D.
700 1 $aVIANELLO, R. P.
700 1 $aBRONDANI, C.
773 $tAustralian Journal of Crop Science$gv. 10, n. 9, p. 1266-1271, 2016.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
27/09/1995 |
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Data da última atualização: |
27/08/2013 |
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Autoria: |
CORRÊA, M. P. F.; STOLBERG W. A. G. Z. |
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Título: |
Propagação vegetativa do guaranazeiro (Paullinia cupana) var. Sorbilis (Mart. Ducke). |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Manaus: EMBRAPA-UEPAE de Manaus, 1981. |
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Páginas: |
4 p. |
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Série: |
(EMBRAPA-UEPAE de Manaus. Pesquisa em Andamento, 23). |
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Idioma: |
Português |
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Conteúdo: |
O estudo impõe-se à pesquisa como prioridade, apoiando os trabalhos de seleção e melhoramento, e na definição de um sistema de produção de mudas pelo processo assexuado compatível com as condições do produtor. |
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Thesagro: |
Guaraná; Paullinia Cupana; Propagação Vegetativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/83059/1/Pesquisa-23-1981.pdf
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Marc: |
LEADER 00777nam a2200181 a 4500
001 1672829
005 2013-08-27
008 1981 bl uuuu u0uu1 u #d
100 1 $aCORRÊA, M. P. F.
245 $aPropagação vegetativa do guaranazeiro (Paullinia cupana) var. Sorbilis (Mart. Ducke).
260 $aManaus: EMBRAPA-UEPAE de Manaus$c1981
300 $a4 p.
490 $a(EMBRAPA-UEPAE de Manaus. Pesquisa em Andamento, 23).
520 $aO estudo impõe-se à pesquisa como prioridade, apoiando os trabalhos de seleção e melhoramento, e na definição de um sistema de produção de mudas pelo processo assexuado compatível com as condições do produtor.
650 $aGuaraná
650 $aPaullinia Cupana
650 $aPropagação Vegetativa
700 1 $aSTOLBERG W. A. G. Z.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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