Data/hora: 04/06/2024 08:56:07

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 18/07/2022
Data da última atualização: | 31/01/2023
Autoria: | MUDO, L. M. D.; QUEIROZ, A. F. S.; MELO, N. F. de; BARBOSA, M. A. G.; ANDRADE, E. C. de; BRITTO, D. de.
Afiliação: | LAISLANE MIRELA DESMONDE MUDO, UNIVASF; AGDA FREIRE SILVA QUEIROZ, IF SERTÃO de Pernambuco; NATONIEL FRANKLIN DE MELO, CPATSA; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DOUGLAS DE BRITTO, CPATSA.
Título: | Stability evaluation of dsRNA and DNA encapsulated in chitosan nanoparticles;
Ano de publicação: | 2022
Fonte/Imprenta: | BioNanoScience, v. 12, p. 774-784, 2022.
DOI: | https://doi.org/10.1007/s12668-022-01003-y
Idioma: | Inglês
Conteúdo: | The polyelectrolyte efect of polysaccharides can be exploited in biotechnological systems for the encapsulation and transport of nucleic acids (NA). The main target applications could be non-viral vaccines and gene silencing. As general application models, this study used plasmid DNA from Xanthomonas citri pv. viticola and dsRNA molecules. Encapsulation occurred by ionotropic gelation based on the pre- and post-addition of NA to the nanoparticle. The suspensions were characterized by UV?visible spectroscopy, electrophoresis, particle size and zeta potential (dynamic light scattering, DLS), and scanning electronic microscopy (SEM). Size distribution was around 173 nm and 587 nm, respectively, for dsRNA and DNA, according to the DLS technique. The post-addition process resulted in small nanoparticles and a more stable suspension, with high zeta potential. The SEM analysis showed spherical nanoparticle and homogeneity in distribution. The degradation study showed that the encapsulation of both dsRNA and DNA resulted in stable nanoparticles when subjected to 80 °C, ultraviolet light, and the action of the RNase enzyme (in the case of dsRNA). Furthermore, stability was still efective in the solid state through the freeze-drying and casting processes. Therefore, dsRNA and DNA nanoencapsulated by ionotropic gelation are feasible, with a high potential for phytopathological application in disease treatment and pest control.
Palavras-Chave: | Cancro bacteriano da videira; Degradação de RNA e DNA; Efeito elicitor; Encapsulamento; Nanoparticula; Nanotecnologia; Quitosana.
Thesagro: | Cancro Bacteriano; Doença; Uva; Xanthomonas Campestris.
Thesaurus Nal: | Encapsulation; Grapes.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (59913-1) - UPC - AP - DD -  -  -  - DIS