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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
04/02/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, L. de C.; CHAGAS, E. C.; CRESCENCIO, R.; PESSOA, M. A.; SILVA, A. L. F. da; CARVALHO, E. da S.; ANDRADE JUNIOR, G. de; BRITO, M. V. T. de; PORTO, M. S. A. do. |
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Afiliação: |
Levy de Carvalho Gomes; EDSANDRA CAMPOS CHAGAS, CPAA; ROGER CRESCENCIO, CPAA; MARCIA ASSUNCAO PESSOA, CPAA; André Luiz Ferreira da Silva; Edivânia da Silva Carvalho; Gilberto de Andrade Junior; Marcus Vinicius Tôrres de Brito; Mahatma Sonhará Araújo do Porto. |
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Título: |
Validation of a simple portable instrument for measurement of blood glucose in four Amazon fishes. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Journal of Aquaculture in the Tropics, New York, v. 20, n. 2, p. 101-109, 2005. |
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Idioma: |
Português |
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Conteúdo: |
Responses of four Amazon fishes (pirarucu, tambaqui, matrinxã and tamoatá) to capture, handling and crowling stress were used to validade the efficcacy of a portable meter in measuring blood glucose. Blood glucose was highly correlated with reference method in all the studied tamoatá. Blood glucose in pirarucu and matrinxã were lower than those obtained with a plasma enzymatics reference method. Reliability of both methods are high being greater in the reference method. The variation of a same sample of blood glucose in resting fish than in stressed fish. These findings indicate a potential role for portable glucose instrument in the field and hatchery facilities, but the result is species specific and should be validated for each species before use. |
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Palavras-Chave: |
Blood glucose meter; Glucosa sanguínea; Medidor de glicose; Medidor portátil; Peixe amazônico; Pez de agua dulce; Portable meter. |
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Thesagro: |
Arapauma gigas; Brycon; Colossoma macropomum; Glicose; Hoplosternum littorale; Matrinxã; Peixe de água doce; Pirarucu; Tambaqui; Tamuatá. |
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Thesaurus Nal: |
Blood glucose; Freshwater fish. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02050naa a2200445 a 4500
001 2119800
005 2020-02-04
008 2005 bl uuuu u00u1 u #d
100 1 $aGOMES, L. de C.
245 $aValidation of a simple portable instrument for measurement of blood glucose in four Amazon fishes.$h[electronic resource]
260 $c2005
520 $aResponses of four Amazon fishes (pirarucu, tambaqui, matrinxã and tamoatá) to capture, handling and crowling stress were used to validade the efficcacy of a portable meter in measuring blood glucose. Blood glucose was highly correlated with reference method in all the studied tamoatá. Blood glucose in pirarucu and matrinxã were lower than those obtained with a plasma enzymatics reference method. Reliability of both methods are high being greater in the reference method. The variation of a same sample of blood glucose in resting fish than in stressed fish. These findings indicate a potential role for portable glucose instrument in the field and hatchery facilities, but the result is species specific and should be validated for each species before use.
650 $aBlood glucose
650 $aFreshwater fish
650 $aArapauma gigas
650 $aBrycon
650 $aColossoma macropomum
650 $aGlicose
650 $aHoplosternum littorale
650 $aMatrinxã
650 $aPeixe de água doce
650 $aPirarucu
650 $aTambaqui
650 $aTamuatá
653 $aBlood glucose meter
653 $aGlucosa sanguínea
653 $aMedidor de glicose
653 $aMedidor portátil
653 $aPeixe amazônico
653 $aPez de agua dulce
653 $aPortable meter
700 1 $aCHAGAS, E. C.
700 1 $aCRESCENCIO, R.
700 1 $aPESSOA, M. A.
700 1 $aSILVA, A. L. F. da
700 1 $aCARVALHO, E. da S.
700 1 $aANDRADE JUNIOR, G. de
700 1 $aBRITO, M. V. T. de
700 1 $aPORTO, M. S. A. do
773 $tJournal of Aquaculture in the Tropics, New York$gv. 20, n. 2, p. 101-109, 2005.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
04/09/2019 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OKINO, C. H.; GIGLIOTI, R.; BASSETTO, C. C.; SILVA, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Rodrigo Giglioti, Instituto de Zootecnia; César Cristiano Bassetto, UNESP; Pamella Cristini Silva, UNICEP; Henrique Nunes de Oliveira, Instituto de Zootecnia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Elimination of erroneous results related to bovine mononuclear cell immunophenotyping by antibodies binding to Fc receptors. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Veterinary Immunology and Immunopathology, v. 213, jul. 2019. |
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DOI: |
https://doi.org/10.1016/j.vetimm.2019.109889 |
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Idioma: |
Inglês |
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Conteúdo: |
Blocking immunoglobulin G (IgG) binding receptors on leukocytes is an established and highly recommended preventive procedure for immunological assays. Failing to prevent such nonspecific binding can lead to erroneous results. Several studies testing different blocking reagents have been performed in murine or human cells, however, there are no specific studies on bovine cells. Our study aimed to investigate the efficiency of blocking reagents to inhibit the nonspecific binding of mouse monoclonal antibodies (mAbs) to bovine peripheral blood cells. We observed nonspecific interactions of IgG2a and IgG2b negative isotypes with bovine leukocytes, but not IgG1. We found that these nonspecific bindings could be eliminated by blocking with purified mouse IgG, whereas little or no blocking effect was observed when bovine serum or Mouse Seroblock FcR were applied. Moreover, in the absence of an efficient blocking reagent, the percentage of CD335 positive cells was significantly higher than in the group previously blocked with mouse IgG. Based on these results, and due to the lack of specific commercial blocking reagents for bovine cells, our recommendation is to use purified mouse IgG as a blocking reagent for immune assays targeting bovine leukocytes in order to enhance the accuracy of the results. |
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Palavras-Chave: |
Ensaios imunológicos; Fc receptor blocking; Mouse IgG; Nonspecific antibody binding; RPE-Cy5. |
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Thesaurus NAL: |
Flow cytometry. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02174naa a2200265 a 4500
001 2111876
005 2023-03-13
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetimm.2019.109889$2DOI
100 1 $aOKINO, C. H.
245 $aElimination of erroneous results related to bovine mononuclear cell immunophenotyping by antibodies binding to Fc receptors.$h[electronic resource]
260 $c2019
520 $aBlocking immunoglobulin G (IgG) binding receptors on leukocytes is an established and highly recommended preventive procedure for immunological assays. Failing to prevent such nonspecific binding can lead to erroneous results. Several studies testing different blocking reagents have been performed in murine or human cells, however, there are no specific studies on bovine cells. Our study aimed to investigate the efficiency of blocking reagents to inhibit the nonspecific binding of mouse monoclonal antibodies (mAbs) to bovine peripheral blood cells. We observed nonspecific interactions of IgG2a and IgG2b negative isotypes with bovine leukocytes, but not IgG1. We found that these nonspecific bindings could be eliminated by blocking with purified mouse IgG, whereas little or no blocking effect was observed when bovine serum or Mouse Seroblock FcR were applied. Moreover, in the absence of an efficient blocking reagent, the percentage of CD335 positive cells was significantly higher than in the group previously blocked with mouse IgG. Based on these results, and due to the lack of specific commercial blocking reagents for bovine cells, our recommendation is to use purified mouse IgG as a blocking reagent for immune assays targeting bovine leukocytes in order to enhance the accuracy of the results.
650 $aFlow cytometry
653 $aEnsaios imunológicos
653 $aFc receptor blocking
653 $aMouse IgG
653 $aNonspecific antibody binding
653 $aRPE-Cy5
700 1 $aGIGLIOTI, R.
700 1 $aBASSETTO, C. C.
700 1 $aSILVA, P. C.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tVeterinary Immunology and Immunopathology$gv. 213, jul. 2019.
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Embrapa Pecuária Sudeste (CPPSE) |
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