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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/11/2010 |
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Data da última atualização: |
29/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; F. D. GUERRERO, LIVESTOCK INSECTS RESEARCH LABORATORY/KERRVILLE-TX. |
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Título: |
Pasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010. |
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Páginas: |
p. 125 - 126. |
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Idioma: |
Português |
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Conteúdo: |
Knockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated. MenosKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each ... Mostrar Tudo |
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Palavras-Chave: |
Allele characterization; KDR; Pyrethroid resistance; Rondonia. |
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Thesaurus Nal: |
Rhipicephalus microplus. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03025nam a2200217 a 4500
001 1865776
005 2023-06-29
008 2010 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aPasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV$c2010
300 $ap. 125 - 126.
520 $aKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated.
650 $aRhipicephalus microplus
653 $aAllele characterization
653 $aKDR
653 $aPyrethroid resistance
653 $aRondonia
700 1 $aBARBIERI, F. da S.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGUERRERO, F. D.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 209 | |
| 61. |  | BEZERRA, W. S. M.; LACERDA, C. L.; RAMOS, M. B.; BRITO, L. G.; BARBIERI, F. da S. Avaliação de transmissão congênita de Babesia bovis e Babesia bigemina por meio de esfregaços sanguíneos. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 8.; ENCONTRO DE PÓS-GRADUAÇÃO, 3., 2017, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2017. p. 68.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 62. | | BRITO, L. G.; BARBIERI, F. da S.; GIGLIOTI, R.; SOUZA, G. C. R.; BENITEZ, F.; OLIVEIRA, M. C. de S. Babesia bovis infection in catlle from Rondonia, Brazil. In: WORLD BUATRICS CONGRESS, 26., 2010, Santiago. Abstracts...Santiago: BUATRICS, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Rondônia. |
|  |
| 63. | | BRITO, L. G.; GASPARIN, G.; HUACCA, M. E. F.; REGITANO, L. C. de A.; BORJA, G. E. M. Avaliação de diferentes protocolos de extração de DNA celular a partir de adultos de Haematobia irritans (Linnaeus, 1758) (Diptera muscidae). In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA 12., 2002, Rio de Janeiro, RJ. Anais... Rio de Janeiro: Colégio Brasileiro de Parasitóligia Veterinária, 2002. 2 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 64. | | FUNES-HUACCA, M.E.; GIGLIOTI, R.; NÉO, T. A.; CARRILHO, E.; BRITO, L. G.; OLIVEIRA, M. C. de S. ANÁLISE DO PERFIL PROTÉICO DOS PRODUTOS DE EXCREÇÃO/SECREÇÃO DE LARVAS DE Cochliomyia hominivorax. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUDESTE, 2006, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 68. | | BARBIERI, F. S.; BRITO, L. G.; LABRUNA, M. B.; BARROS-BATTESTI, D. M.; CAMARGO, L. M. A.; FAMADAS, K. M. Description of the larva of Amblyoma ovale Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy. Systematic and Applied Acarology, v. 13, p. 109-119, 2008. Meta 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Rondônia. |
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| 69. | | BARBIERI, F. S.; BRITO, L. G.; LABRUNA, M. B.; BARROS-BATTESTI, D. M.; CAMARGO, L. M. A.; FAMADAS, K. M. Description of the larva of Amblyomma pacae Aragão, 1911 (Acari: Ixodidae) by light and scanning electron microscopy. Systematic and Applied Acarology, v. 13, p. 195-203, 2008. Metas 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Rondônia. |
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| 70. | | MATOS, A. S.; FIGUEIRO, M. R.; BRITO, L. G.; MARCONDES, C. R.; MARQUES, J. R. F.; PAZ, C. C. da. Desenvolvimento ponderal e correlação com produção de leite em rebanho de búfalas sob controle leiteiro no estado de Rondônia. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 46., 2009, Maringá. Inovação científica e tecnológica em zootecnia: anais dos resumos. Maringa: SBZ: UEM, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 71. | | FERREZINI, J.; SCHIAVONE, D. C.; BRITO, L. G.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Diagnóstico da resistência de Rhipicephalus (Boophilus) microplus a carrapaticidas no rebanho bovino da Embrapa Pecuária Sudeste. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUDESTE, 2., 2007, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste, 2007. (Embrapa Pecuária Sudeste. Documentos, 76).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 75. | | MAROSO, R. D.; BARBIERI, F. da S.; BRITO, L. G.; FIGUEIRO, M. R.; BANDEIRA, P. F.; LANZONI, M. M. Identificação das espécies do gênero Eimeria em bezerros bovinos e bubalinos criados em Rondônia. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande. [Anais...] Campo Grande: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 78. | | BRITO, L. G.; REGITANO, L. C. de A.; OLIVEIRA, M. C. de S.; HUACCA, M. E. F.; BORJA, M. Genotype characterization of the Haematobia irritans (Diptera: muscidae) from Brazil, Dominican Republic and Clombia based on Randomly amplied polymorphic DNA (RAPD) analysis. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 14.; SIMPÓSIO LATINO-AMERICANO DE RICKETTSIOSES, 2., 2006, Ribeirão Preto. Anais... Ribeirão Preto: CBPV, 2006. p. 298| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 79. | | BRITO, L. G.; REGITANO, L. de A.; HUACCA, M. E. F.; CARRILHO, E.; PAES, M. J.; MOYA-BORJA, G. Genotype characterization of the Haematobia irritans (Diptera: muscidae) from Brazil, Dominican Republic and Colombia based on randomly amplified polymorphic DNA (RADP) analysis. Revista Brasileira de Parasitologia Veterinária, v. 17, n. 4, p. 179-184, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Rondônia. |
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| 80. | | BRITO, L. G.; REGITANO, L. C. de A.; OLIVEIRA, M. C. de S.; HUACCA, M. E. F.; BORJA, M. Genotype characterization of the Haematobia irritans (Diptera: muscidae) from different Brazilian geographic regions based on Randomly amplied polymorphic DNA (RAPD) analysis. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 14.; SIMPÓSIO LATINO-AMERICANO DE RICKETTSIOSES, 2., 2006, Ribeirão Preto. Anais... Ribeirão Preto: CBPV, 2006. p. 183.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 209 | |
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