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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
Data corrente: |
04/09/2017 |
Data da última atualização: |
16/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRITO, L. G.; NERY, L. de O.; BARBIERI, F. da S.; HUACCA, M. E. F.; PEREIRA, S. dos S.; SILVA, R. R. da; FERNANDES, C. F. C.; OLIVEIRA, M. C. de S. |
Afiliação: |
LUCIANA GATTO BRITO, CPATU; Louí de Oliveira Néry, Bolsista CPATU; FABIO DA SILVA BARBIERI, CPATU; Maribel Elizabeth Funes Huacca, UNIR; Soraya dos Santos Pereira, Fiocruz Rondônia; RENATA REIS DA SILVA, CPATU; Carla Freire Celedonio Fernandes, Fiocruz Rondônia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Molecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 5, p. 725-732, 2017. |
DOI: |
https://doi.org/10.1016/j.ttbdis.2017.05.006 |
Idioma: |
Inglês |
Conteúdo: |
The use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. MenosThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future ... Mostrar Tudo |
Palavras-Chave: |
Cattle tick; Organophosphate resistance. |
Thesaurus Nal: |
esterases. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02493naa a2200253 a 4500 001 2076221 005 2019-01-16 008 2017 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ttbdis.2017.05.006$2DOI 100 1 $aBRITO, L. G. 245 $aMolecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus.$h[electronic resource] 260 $c2017 520 $aThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. 650 $aesterases 653 $aCattle tick 653 $aOrganophosphate resistance 700 1 $aNERY, L. de O. 700 1 $aBARBIERI, F. da S. 700 1 $aHUACCA, M. E. F. 700 1 $aPEREIRA, S. dos S. 700 1 $aSILVA, R. R. da 700 1 $aFERNANDES, C. F. C. 700 1 $aOLIVEIRA, M. C. de S. 773 $tTicks and Tick-borne Diseases$gv. 8, n. 5, p. 725-732, 2017.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/09/2012 |
Data da última atualização: |
10/11/2016 |
Tipo da produção científica: |
Documentos |
Autoria: |
CASTRO, M. E. B. de; RIBEIRO, Z. M. de A.; SOUZA, M. L. de; SIHLER, W.; ESTEVES, P. A.; LAZZAROTTI, M. |
Afiliação: |
MARIA ELITA BATISTA DE CASTRO, CENARGEN; ZILDA MARIA DE ARAUJO RIBEIRO, CENARGEN; MARLINDA LOBO DE SOUZA, CENARGEN; WILLIAM SIHLER, CENARGEN; PAULO AUGUSTO ESTEVES, CNPSA; MATEUS LAZZAROTTI, CNPSA. |
Título: |
Manual de curadores de germoplasma - microorganismos: vírus de invertebrados, suínos e aves. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2011. |
Páginas: |
20 P. |
ISSN: |
(Embrapa Recursos Genéticos e Biotecnologia. Documentos, 331; Embrapa Suínos e Aves. Documentos, 152). |
Idioma: |
Português |
Palavras-Chave: |
Ave. |
Thesagro: |
Animal invertebrado; Conservação; Microrganismo; Recurso genético; Suíno; Vírus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/149778/1/doc331-152.pdf
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Marc: |
LEADER 00866nam a2200265 a 4500 001 1933494 005 2016-11-10 008 2011 bl uuuu u0uu1 u #d 022 $a(Embrapa Recursos Genéticos e Biotecnologia. Documentos, 331; Embrapa Suínos e Aves. Documentos, 152). 100 1 $aCASTRO, M. E. B. de 245 $aManual de curadores de germoplasma - microorganismos$bvírus de invertebrados, suínos e aves.$h[electronic resource] 260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2011 300 $a20 P. 650 $aAnimal invertebrado 650 $aConservação 650 $aMicrorganismo 650 $aRecurso genético 650 $aSuíno 650 $aVírus 653 $aAve 700 1 $aRIBEIRO, Z. M. de A. 700 1 $aSOUZA, M. L. de 700 1 $aSIHLER, W. 700 1 $aESTEVES, P. A. 700 1 $aLAZZAROTTI, M.
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