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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
04/11/2011 |
Data da última atualização: |
21/12/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CONCENCO, G.; SALTON, J. C.; SECRETTI, M. L.; MENDES, P. B.; BREVILIERI, R. C.; GALON, L. |
Afiliação: |
GERMANI CONCENCO, CPAO; JULIO CESAR SALTON, CPAO; M. L. SECRETTI, UEMS; P. B. MENDES, UNIGRAN; R. C. BREVILIERI, UEMS; L. GALON, UNIPAMPA. |
Título: |
Effect of long-term agricultural management systems on occurrence and composition of weed species. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Planta Daninha, Viçosa, v. 29, n. 3, p. 515-522, 2011. |
Idioma: |
Português |
Palavras-Chave: |
Fitossociologia; Integração lavoura-pecuária. |
Thesagro: |
Sistema de Cultivo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45556/1/RPD-v29-n3-Concenco-Salton-1.pdf
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Marc: |
LEADER 00625naa a2200205 a 4500 001 1905017 005 2011-12-21 008 2011 bl uuuu u00u1 u #d 100 1 $aCONCENCO, G. 245 $aEffect of long-term agricultural management systems on occurrence and composition of weed species. 260 $c2011 650 $aSistema de Cultivo 653 $aFitossociologia 653 $aIntegração lavoura-pecuária 700 1 $aSALTON, J. C. 700 1 $aSECRETTI, M. L. 700 1 $aMENDES, P. B. 700 1 $aBREVILIERI, R. C. 700 1 $aGALON, L. 773 $tPlanta Daninha, Viçosa$gv. 29, n. 3, p. 515-522, 2011.
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Embrapa Agropecuária Oeste (CPAO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
10/08/2004 |
Data da última atualização: |
10/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
SILVA, J. R. V.; HURK, R. van den; COSTA, S. H. F.; ANDRADE, E. R.; NUNES, A. P. A.; FERREIRA, F. V. A.; LOBO, R. N. B.; FIGUEIREDO, J. R. |
Afiliação: |
JOSÉ R. V. SILVA, UNIVERSIDADE ESTADUAL DO CEARÁ; ROBERT VAN DEN HURK, UTRECHT UNIVERSITY; SONIA H. F. COSTA, UNIVERSIDADE ESTADUAL DO CEARÁ; EVELYN R. ANDRADE, UNIVERSIDADE ESTADUAL DO CEARÁ; RAIMUNDO NONATO BRAGA LOBO, CNPC. |
Título: |
Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 81, n. 3/4, p. 273-286, 2004. |
DOI: |
10.1016/j.anireprosci.2003.09.006 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration. MenosAbstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles... Mostrar Tudo |
Palavras-Chave: |
Activation; Drug effects; Folículo ovariano; Folículo primordial; Growth; Maintenance; Ovary; Primordial follicles; Suplemento; Supplements. |
Thesagro: |
Água de Coco; Caprino; Ovário; Reprodução. |
Thesaurus NAL: |
Bovine serum albumin; Cell division; Coconut water; Females; Glutamine; Goats; Granulosa cells; Hypoxanthine; In vitro culture; Insulin; Oocytes; Ovarian follicles; Pharmacology; Physiology; Reproduction; Solutions; Transferrin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03757naa a2200589 a 4500 001 1530666 005 2024-04-10 008 2004 bl uuuu u00u1 u #d 024 7 $a10.1016/j.anireprosci.2003.09.006$2DOI 100 1 $aSILVA, J. R. V. 245 $aSurvival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water.$h[electronic resource] 260 $c2004 520 $aAbstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration. 650 $aBovine serum albumin 650 $aCell division 650 $aCoconut water 650 $aFemales 650 $aGlutamine 650 $aGoats 650 $aGranulosa cells 650 $aHypoxanthine 650 $aIn vitro culture 650 $aInsulin 650 $aOocytes 650 $aOvarian follicles 650 $aPharmacology 650 $aPhysiology 650 $aReproduction 650 $aSolutions 650 $aTransferrin 650 $aÁgua de Coco 650 $aCaprino 650 $aOvário 650 $aReprodução 653 $aActivation 653 $aDrug effects 653 $aFolículo ovariano 653 $aFolículo primordial 653 $aGrowth 653 $aMaintenance 653 $aOvary 653 $aPrimordial follicles 653 $aSuplemento 653 $aSupplements 700 1 $aHURK, R. van den 700 1 $aCOSTA, S. H. F. 700 1 $aANDRADE, E. R. 700 1 $aNUNES, A. P. A. 700 1 $aFERREIRA, F. V. A. 700 1 $aLOBO, R. N. B. 700 1 $aFIGUEIREDO, J. R. 773 $tAnimal Reproduction Science$gv. 81, n. 3/4, p. 273-286, 2004.
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