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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/05/2017 |
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Data da última atualização: |
29/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. |
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Afiliação: |
Carolina Cerqueira Sarmento Olivares; Joanna Maria Gonçalves de Souza-Fabjan; JEFERSON FERREIRA DA FONSECA, CNPC; Mario Felipe Alvarez Balaro; Vicente José de Figueirêdo Freitas; Rodrigo Vasconcelos de Oliveira; Felipe Zandonadi Brandão. |
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Título: |
Comparison of different sperm selection techniques in ram frozen-thawed sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 45, p. 1431, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique. MenosResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of inte... Mostrar Tudo |
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Palavras-Chave: |
CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques; Sperm preparation. |
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Thesagro: |
Espermatozóide; Ovino; Reprodução animal; Sêmen. |
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Thesaurus Nal: |
Reproduction; Sheep; sperm capacitation; Spermatozoa; Treaties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160322/1/CNPC-2017-Comparison.pdf
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Marc: |
LEADER 04855naa a2200373 a 4500
001 2070178
005 2017-05-29
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different sperm selection techniques in ram frozen-thawed sperm.
260 $c2017
520 $aResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique.
650 $aReproduction
650 $aSheep
650 $asperm capacitation
650 $aSpermatozoa
650 $aTreaties
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSêmen
653 $aCASA
653 $aCTC
653 $aPlasma membrane integrity
653 $aProtocolo
653 $aReproductive techniques
653 $aSperm preparation
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aFREITAS, V. J. de F.
700 1 $aOLIVEIRA, R. V. de
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 45, p. 1431, 2017.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 263 | |
| 121. |  | LIMA, J. T. M.; FONSECA, J. F. da; BALARO, M. F. A.; RIBEIRO, A. C. S.; DELGADO, K. F.; BRANDÃO, F. Z. Taxa de recuperação de estruturas em ovelhas da raça Santa Inês após protocolo de superovulação. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. SBTE 071. p. 364.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 122. | | ESTEVES, L. V.; BRANDÃO, F. Z.; ZAMBRINI, F. N.; SIQUEIRA, L. G. B.; FERREIRA, M. I. C.; FONSECA, J. F. da. Superovulação e coleta de embriões pela via transcervical em cabras da raça Toggenburg durante a estação reprodutiva. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 20., 2013, Uberlandia. Anais... Belo Horizonte: CBRA, 2013. p. 57.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 123. | | ESTEVES, L. V.; BRANDÃO, F. Z.; ZAMBRINI, F. N.; SIQUEIRA, L. G. B.; FERREIRA, M. I. C.; FONSECA, J. F. da. Superovulação e coleta de embriões pela via transcervical em cabras da raça Toggenburg durante a estação reprodutiva. CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 20., 2013, Uberlandia. Anais... Belo Horizonte: CBRA, 2013. p. 57| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 124. | | FONSECA, J. F. da; ESTEVES, L. V.; ZAMBRINI, F. N.; FERREIRA, M. I. C.; SIQUEIRA, L. G. B.; BRANDÃO, F. Z. Superovulation and embryo recovery by transcervical technique in Toggenburg goats during seasonal anestrus. In: ANNUAL MEETING - AETE, 29., 2013, Istanbul, Turkey. Colloque cientifique. Istanbu: Association Europeenne de Transfert Embryonnaire, 2013. p. 130.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 125. | | MAIA, A. L. R. S.; BRANDAO, F. Z.; SANTOS, G. B.; BALARO, M. F. A.; RODRIGUES, A. L. R.; FONSECA, J. F. da; FACO, O. Use of D-Cloprostenol at different intervals of administration for estrous synchronization in cyclic Saanen goats. Animal Reproduction, v. 11, n. 3, p. 338, Jul./Sept. 2014. Abstract A058. Proceedings of the 28th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August, 2014, Natal, RN, Brazil. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 126. | | PRATES, J. F.; BRAIR, V. L.; MAIA, A. L. R. S.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; BONATO, G. C.; FONSECA, J. F. da. Use of human intravaginal tampon embedded with natural progesterone induces synchronous estrus in Santa Inês ewes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 71, n. 1, p. 345-348, 2019.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 127. | | PINTO NETO, A.; LUCCA, F. M. de; ALBERTON, J.; MOTA, M. F.; LUCACIN, E.; BORGES, A. M.; ACCO, A.; FONSECA, J. F. da; SILVA FILHO, J. M. da; SILVA, A. V. da; BRANDÃO, F. Z. Avaliação dos efeitos do flunixim meglumine sobre a concentração sérica de progesterona e ciclo estral em novilhas e vacas mestiças. Revista Brasileira de Ciência Veterinária, v. 15, n. 1, p. 10-14, jan./abr. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 128. | | CORTÊS, L. R.; FRAGA, M. O.; SILVA, D. S.; PRATES, J. F.; MARTINS, B. B.; FIGUEIRA, L. M.; BONATO, G. C.; ARRAIS, A. M.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; FONSECA, J. F. da. Administration of HCG seven days after estrous onset increases the pregnancy rate in Toggenburg goats subjected to induction of synchronized estrus and natural mating. Animal Reprodroduction, v. 15, n. 3, p. 366, Jul./Sept. 2018. Abstract A051. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 129. | | CÔRTES, L. R.; SOUZA-FABJAN, J. M. G.; DIAS, D. S.; MARTINS, B. B.; MAIA, A. L. R. S.; VEIGA, M. O.; ARASHIRO, E. K. N.; BRANDÃO, F. Z.; OLIVEIRA, M. E. F.; BARTLEWSKI, P. M.; FONSECA, J. F. da. Administration of a single dose of 300 IU of human chorionic gonadotropin seven days after the onset of estrus improves pregnancy rate in dairy goats by an unknown mechanism. Domestic Animal Endocrinology, v. 74, e106579, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 130. | | SOUZA, J. M. G.; TORRES, C. A. A.; MAIA, A. R. L. S.; BRANDÃO, F. Z.; BRUSCHI, J. H.; VIANA, J. H. M.; OBÁ, E.; FONSECA, J. F. da. Autoclaved, previously used intravaginal progesterone devices induces estrus and ovulation in anestrous Toggenburg goats. Animal Reproduction Science, v. 129, n. 1/2, p. 50-55, Nov. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 131. | | PINTO, P. H. N.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; RIBEIRO, L. dos S.; BRAGANÇA, G. M.; LEITE, C. R.; ARASHIRO, E. K. N.; SILVA, K. de M.; FONSECA, J. F. da; BRANDÃO, F. Z. Anti-Müllerian hormone and antral follicle count are more effective for selecting ewes with good potential for in vivo embryo production than the presence of FecGE mutation or eCG pre-selection tests. Theriogenology, v. 113, p. 146-152, Feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 132. | | SANTOS, V. M. B. dos; BALARO, M. F. A.; SANTOS, J. D. R.; TAIRA, A. R.; PEREIRA, P. V. dos S.; GONÇALVES, F. M.; FONSECA, J. F. da; BRANDÃO, F. Z. Apenas o uso da ocitocina é eficiente para a transposição cervical visando a coleta de embrião não cirúrgica em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 502, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 133. | | ARASHIRO, E. K. N.; VIANA, J. H. M.; FONSECA, J. F. da; SIQUEIRA, L. G. B.; BRUSCHI, J. H.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; BRANDAO, F. Z. Assessment of luteal function in Toggenburg goats by computer-assisted image analysis. Reproduction, Fertility and Development, v.21, n. 1, p. 189, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 134. | | ARASHIRO, E. K. N.; VIANA, J. H. M.; FONSECA, J. F. da; SIQUEIRA, L. G. B.; BRUSCHI, J. H.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; BRANDAO, F. Z. Assessment of Luteal function in toggenbutg Goats by computer-assisted image analysis. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceedings... San Diego: ISTE, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 135. | | SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 199.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 136. | | PINTO, P. H. N.; BALARO, M. F. A.; RIBEIRO, A. C. S.; DELGADO, K. F.; FERNANDES, D. A. de M.; CAVALCANTI, L. M.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação de dois protocolos hormonais a base de d-cloprostenol para sincronização do estro em ovelhas leiteiras da raça Lacaune em estação reprodutiva no rio de Janeiro. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 186.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 137. | | VARAGO, F. C.; MOUTACAS, V. S.; CARVALHO, B. C. de; SERAPIÃO, R. V.; VIEIRA, F.; CHIARINI-GARCIA, H.; BRANDÃO, F. Z.; CAMARGO, L. S. de A.; HENRY, M.; LAGARES, M. A. Comparison of conventional freezing and vitrification with dimethylformamide and ethylene glycol for cryopreservation of ovine embryos. Reproduction in Domestic Animals, v. 49, n. 5, p. 839-44, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 138. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. Comparison of different sperm selection techniques in ram frozen-thawed sperm. Acta Scientiae Veterinariae, v. 45, p. 1431, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 139. | | SANTOS, J. D. R.; OLIVEIRA, I. C.; BRAIR, V. L.; BALARO, M. F. A.; MORAIS, M. C. C.; ARASHIRO, E. K. N.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BRANDÃO, F. Z. Comparison of stress and animal welfare caused by the procedures of embryo collection by either surgical or non-surgical via in sheep. Animal Reprodroduction, v. 15, n. 3, p. 404, Jul./Sept. 2018. Abstract A091. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 140. | | LIMA, J. M. T.; FONSECA, J. F. da; BALARO, M. F. A.; RIBEIRO, A. C. S.; RODRIGUES, C. A. F.; TORRES FILHO, R. A.; ESTEVES, L. V.; BRANDÃO, F. Z. Comportamento sexual de ovelhas superovuladas submetidas a dois métodos de acasalamento. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 20., 2013, Uberlandia. Anais... Belo Horizonte: CBRA, 2013. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 263 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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