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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
05/12/2016 |
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Data da última atualização: |
07/12/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BRIGIDA, A. I. S.; SILVA, C. M.; STEPHAN, M. P.; ARAUJO, C. A.; SILVA, K. N.; GOTTSCHALK, L. M. F. |
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Afiliação: |
ANA IRAIDY SANTA BRIGIDA, CTAA; CAROLINE MELLINGER SILVA, CTAA; MARILIA PENTEADO STEPHAN, CTAA; Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro; Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro; LEDA MARIA FORTES GOTTSCHALK, CTAA. |
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Título: |
Imobilização de lipases por adsorção em agarose modificada. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida. Anais. Gramado: SBCTA Regional, 2016. |
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Páginas: |
6 p. |
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Idioma: |
Português |
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Notas: |
Food: the tree that sustains life. 913. |
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Conteúdo: |
Buscando contribuir para o desenvolvimento de processos contínuos e o reuso de enzimas, bem como promovendo uma maior estabilidade e atividade catalítica de enzimas, muitos estudos vem sendo realizados para obtenção de lipases imobilizadas. Neste trabalho o processo de imobilização de lipases de Aspergillus niger C e de Thermomyces lanuginosus por adsorção em agarose modificada foi avaliado. A imobilização ocorreu a pH 7 em três tipos de suportes a base de agarose: octil sepharose, DEAE sepharose e CM sepharose. O uso de octil sepharose mostrou-se mais eficiente na obtenção de derivados mais ativos frente à DEAE e CM sepharose, independente do tipo de extrato de lipase usado. Maior atividade no derivado foi obtida na imobilização de lipase de T. lanuginosus (304 U/g de octil sepharose) com 1,1 mg de proteína adsorvida em 1 grama de suporte. |
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Palavras-Chave: |
Hydrophobic interaction; Interações hidrofóbicas; Troca iônica. |
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Thesagro: |
Adsorção; Aspergillus Niger. |
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Thesaurus Nal: |
adsorption; ion exchange; Thermomyces lanuginosus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151212/1/913.pdf
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Marc: |
LEADER 01884nam a2200289 a 4500
001 2057913
005 2016-12-07
008 2016 bl uuuu u00u1 u #d
100 1 $aBRIGIDA, A. I. S.
245 $aImobilização de lipases por adsorção em agarose modificada.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida. Anais. Gramado: SBCTA Regional$c2016
300 $a6 p.
500 $aFood: the tree that sustains life. 913.
520 $aBuscando contribuir para o desenvolvimento de processos contínuos e o reuso de enzimas, bem como promovendo uma maior estabilidade e atividade catalítica de enzimas, muitos estudos vem sendo realizados para obtenção de lipases imobilizadas. Neste trabalho o processo de imobilização de lipases de Aspergillus niger C e de Thermomyces lanuginosus por adsorção em agarose modificada foi avaliado. A imobilização ocorreu a pH 7 em três tipos de suportes a base de agarose: octil sepharose, DEAE sepharose e CM sepharose. O uso de octil sepharose mostrou-se mais eficiente na obtenção de derivados mais ativos frente à DEAE e CM sepharose, independente do tipo de extrato de lipase usado. Maior atividade no derivado foi obtida na imobilização de lipase de T. lanuginosus (304 U/g de octil sepharose) com 1,1 mg de proteína adsorvida em 1 grama de suporte.
650 $aadsorption
650 $aion exchange
650 $aThermomyces lanuginosus
650 $aAdsorção
650 $aAspergillus Niger
653 $aHydrophobic interaction
653 $aInterações hidrofóbicas
653 $aTroca iônica
700 1 $aSILVA, C. M.
700 1 $aSTEPHAN, M. P.
700 1 $aARAUJO, C. A.
700 1 $aSILVA, K. N.
700 1 $aGOTTSCHALK, L. M. F.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
17/02/2011 |
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Data da última atualização: |
20/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FURTADO, G. Q.; MORAES, S. R. G.; ALVES, S. A. M.; AMORIM, L.; MASSOLA JUNIOR, N. S. |
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Afiliação: |
GLEIBER QUINTÃO FURTADO, UFV; SYLVIA RAQUEL GOMES MORAES, ESALQ/USP; SILVIO ANDRE MEIRELLES ALVES, CNPUV; LILIAN AMORIM, UFV; NELSON SIDNEI MASSOLA JUNIOR, UFV. |
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Título: |
The infection of soybean leaves by Phakopsora pachyrhizi during conditions of discontinuous wetness. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Phytopathology, Berlin, v. 159, n. 3, p. 165-170, mar. 2011. |
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Volume: |
159 |
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Páginas: |
165-170 |
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DOI: |
10.1111/j.1439-0434.2010.01742.x |
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Idioma: |
Inglês |
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Conteúdo: |
The ability of Phakopsora pachyrhizi to cause infection under conditions of discontinuous wetness was investigated. In in vitro experiments, droplets of a uredospore suspension were deposited onto the surface of polystyrene. After an initial wetting period of either 1, 2 or 4 h, the drops were dried for different time intervals and then the wetness was restored for 11, 10 or 8 h. Germination and appressorium formation were evaluated. In in vivo experiments, soybean plants were inoculated with a uredospore suspension. Leaf wetness was interrupted for 1, 3 or 6 h after initial wetting periods of 1, 2 or 4 h. Then, the wetting was re-established for 11, 10 or 8 h, respectively. Rust severity was evaluated 14 days after inoculation. The germination of the spores and the formation of the appressoria on the soybean leaves after different periods of wetness were also quantified in vivo by scanning electron microscopy. P. pachyrhizi showed a high infective capacity during short periods of time. An interruption of wetness after 1 h caused average reductions in germination from 56 to 75% and in appressorium formation from 84 to 96%. Rust severity was lower in all of the in vivo treatments with discontinuous wetness when compared to the control plants. Rust severity was zero when the interruption of wetness occurred 4 h after the initial wetting. Wetting interruptions after 1 and 2 h reduced the average rust severity by 83 and 77%, respectively. The germination of the uredospores on the soybean leaves occurred after 2 h of wetness, with a maximum germination appearing after 4 h of wetness. Wetness interruption affected mainly the spores that had initiated the germination. MenosThe ability of Phakopsora pachyrhizi to cause infection under conditions of discontinuous wetness was investigated. In in vitro experiments, droplets of a uredospore suspension were deposited onto the surface of polystyrene. After an initial wetting period of either 1, 2 or 4 h, the drops were dried for different time intervals and then the wetness was restored for 11, 10 or 8 h. Germination and appressorium formation were evaluated. In in vivo experiments, soybean plants were inoculated with a uredospore suspension. Leaf wetness was interrupted for 1, 3 or 6 h after initial wetting periods of 1, 2 or 4 h. Then, the wetting was re-established for 11, 10 or 8 h, respectively. Rust severity was evaluated 14 days after inoculation. The germination of the spores and the formation of the appressoria on the soybean leaves after different periods of wetness were also quantified in vivo by scanning electron microscopy. P. pachyrhizi showed a high infective capacity during short periods of time. An interruption of wetness after 1 h caused average reductions in germination from 56 to 75% and in appressorium formation from 84 to 96%. Rust severity was lower in all of the in vivo treatments with discontinuous wetness when compared to the control plants. Rust severity was zero when the interruption of wetness occurred 4 h after the initial wetting. Wetting interruptions after 1 and 2 h reduced the average rust severity by 83 and 77%, respectively. The germination of the uredospores on th... Mostrar Tudo |
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Thesagro: |
Doença de planta; Ferrugem; Folha; Fungo; Soja; Umidade. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/27516/1/SILVIO-JPhytopathol-v159p165-2011.pdf
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Marc: |
LEADER 02488naa a2200277 a 4500
001 1877741
005 2019-05-20
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1439-0434.2010.01742.x$2DOI
100 1 $aFURTADO, G. Q.
245 $aThe infection of soybean leaves by Phakopsora pachyrhizi during conditions of discontinuous wetness.$h[electronic resource]
260 $c2011
300 $a165-170 159
490 $v159
520 $aThe ability of Phakopsora pachyrhizi to cause infection under conditions of discontinuous wetness was investigated. In in vitro experiments, droplets of a uredospore suspension were deposited onto the surface of polystyrene. After an initial wetting period of either 1, 2 or 4 h, the drops were dried for different time intervals and then the wetness was restored for 11, 10 or 8 h. Germination and appressorium formation were evaluated. In in vivo experiments, soybean plants were inoculated with a uredospore suspension. Leaf wetness was interrupted for 1, 3 or 6 h after initial wetting periods of 1, 2 or 4 h. Then, the wetting was re-established for 11, 10 or 8 h, respectively. Rust severity was evaluated 14 days after inoculation. The germination of the spores and the formation of the appressoria on the soybean leaves after different periods of wetness were also quantified in vivo by scanning electron microscopy. P. pachyrhizi showed a high infective capacity during short periods of time. An interruption of wetness after 1 h caused average reductions in germination from 56 to 75% and in appressorium formation from 84 to 96%. Rust severity was lower in all of the in vivo treatments with discontinuous wetness when compared to the control plants. Rust severity was zero when the interruption of wetness occurred 4 h after the initial wetting. Wetting interruptions after 1 and 2 h reduced the average rust severity by 83 and 77%, respectively. The germination of the uredospores on the soybean leaves occurred after 2 h of wetness, with a maximum germination appearing after 4 h of wetness. Wetness interruption affected mainly the spores that had initiated the germination.
650 $aDoença de planta
650 $aFerrugem
650 $aFolha
650 $aFungo
650 $aSoja
650 $aUmidade
700 1 $aMORAES, S. R. G.
700 1 $aALVES, S. A. M.
700 1 $aAMORIM, L.
700 1 $aMASSOLA JUNIOR, N. S.
773 $tJournal of Phytopathology, Berlin$gv. 159, n. 3, p. 165-170, mar. 2011.
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