Data/hora: 22/09/2024 19:51:27

Biblioteca(s): 	Embrapa Agrobiologia; Embrapa Soja.	
Data corrente: 	09/06/2017	
Data da última atualização: 	22/09/2017	
Autoria: 	DALL'AGNOL, R. F; BOURNAUD, C.; FARIA, S. M. de; BÉNA, G.; MOULIN, L.; HUNGRIA, M.	
Afiliação: 	REBECA FUZINATTO DALL'AGNOL, UEL; CAROLINE BOURNAUD, IRD CIRAD; SERGIO MIANA DE FARIA, CNPAB; GILLES BÉNA, IRD CIRAD; LIONEL MOULIN, IRD CIRAD; MARIANGELA HUNGRIA DA CUNHA, CNPSO.	
Título: 	Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).	
Ano de publicação: 	2017	
Fonte/Imprenta: 	FEMS Microbiology Ecology, v. 93, n. 4, Apr. 2017	
DOI: 	http://doi.org.secure.sci-hub.cc/10.1093/femsec/fix027	
Idioma: 	Inglês	
Conteúdo: 	Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called ?-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (?-rhizobia) and Cupriavidus (?-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All ?- and ?-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed.	
Palavras-Chave: 	Beta-rhizobia; Biological nitrogen fixation; Diazotrophic bacteria; Mata Atlântica.	
Thesagro: 	Floresta tropical; Simbiose.	
Thesaurus Nal: 	nodulation; phylogeny; Symbiosis; Tropical forests.	
Categoria do assunto: 	P Recursos Naturais, Ciências Ambientais e da Terra
S Ciências Biológicas	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (40502-1) - UPC - AP - DD - 2017.00051 -  - 2017.00051 - DIS
CNPSO - (37767-1) - UPC - AP - DD -  -  -  - DIS