Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
08/01/2002 |
Data da última atualização: |
08/01/2002 |
Autoria: |
BLAUDEZ, D.; BOTTON, B.; CHALOT, M. |
Afiliação: |
University Henri Poincare. |
Título: |
Cadmium uptake and subcellular compartmentation in the extomycorrhizalfungus Paxillus involutus |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Microbiology, v.146, n.5, p.1109-1117, 2000 |
Idioma: |
Inglês |
Conteúdo: |
Cadmium uptake and subcellular compartmentation in the ectomycorrhizalfungus Paxillus involutus were investigated using radiotracer fkux analyses. Concentration-dependent Cd2+-uptake kinetics were characterizedby a snooth, non-saturating curve that could be dissected into linear and saturable components. The linear-uptake kinetic component was interpreted as representing binding of Cd to apoplastic components, whereas the remaining saturable component was the result of carrier-mediatedtransport across the plasma membrane. Cell-wall-bound Cd was almost completely removed during desorption from cell-wall preparations. Cd2+desorption from intact mycelium was found to be a function of time involving three compartments corresponding in series to cell wall (50%), cytoplasm (30%) and vacuole (20%), when mycelia were exposed to a 0-05uMCd concentration. |
Categoria do assunto: |
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Marc: |
LEADER 01271naa a2200145 a 4500 001 1650329 005 2002-01-08 008 2000 bl --- 0-- u #d 100 1 $aBLAUDEZ, D. 245 $aCadmium uptake and subcellular compartmentation in the extomycorrhizalfungus Paxillus involutus 260 $c2000 520 $aCadmium uptake and subcellular compartmentation in the ectomycorrhizalfungus Paxillus involutus were investigated using radiotracer fkux analyses. Concentration-dependent Cd2+-uptake kinetics were characterizedby a snooth, non-saturating curve that could be dissected into linear and saturable components. The linear-uptake kinetic component was interpreted as representing binding of Cd to apoplastic components, whereas the remaining saturable component was the result of carrier-mediatedtransport across the plasma membrane. Cell-wall-bound Cd was almost completely removed during desorption from cell-wall preparations. Cd2+desorption from intact mycelium was found to be a function of time involving three compartments corresponding in series to cell wall (50%), cytoplasm (30%) and vacuole (20%), when mycelia were exposed to a 0-05uMCd concentration. 700 1 $aBOTTON, B. 700 1 $aCHALOT, M. 773 $tMicrobiology$gv.146, n.5, p.1109-1117, 2000
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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