| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
14/12/2016 |
|
Data da última atualização: |
03/10/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
CARDOSO, A. A.; ANDRAUS, M. de P.; BORBA, T. C. de O.; MARTIN-DIDONET, C. C. G.; FERREIRA, E. P. de B. |
|
Afiliação: |
ALINE ASSIS CARDOSO, UFG; MICHEL DE PAULA ANDRAUS, UFG; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS, Anápolis-GO; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF. |
|
Título: |
Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 48, n. 1, p. 43-50, jan./mar. 2017. |
|
ISSN: |
1678-4405 |
|
DOI: |
10.1016/j.bjm.2016.09.002 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments. MenosThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean i... Mostrar Tudo |
|
Palavras-Chave: |
Carbon source use; Symbiotic efficiency; Toleance to salinity and temperature. |
|
Thesagro: |
Feijão; Phaseolus vulgaris; Rhizobium; Simbiose. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156257/1/CNPAF-2017-bjm.pdf
|
|
Marc: |
LEADER 02432naa a2200277 a 4500
001 2058672
005 2017-10-03
008 2017 bl uuuu u00u1 u #d
022 $a1678-4405
024 7 $a10.1016/j.bjm.2016.09.002$2DOI
100 1 $aCARDOSO, A. A.
245 $aCharacterization of rhizobia isolates obtained from nodules of wild genotypes of common bean.$h[electronic resource]
260 $c2017
520 $aThis study aimed to evaluate the tolerance to salinity and temperature, the genetic diversityand the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of commonbean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The iso-lates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at differenttemperatures (28◦C, 33◦C, 38◦C, 43◦C and 48◦C). Genotypic characterization was performedbased on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbioticefficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to tem-perature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbonsources studied. Clustering analysis based on the ability to use carbon sources and on thetolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropiciH12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolatesshowed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobiumreference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that twoefficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercialinoculant for common bean in Brazil and must be assayed in field experiments.
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aRhizobium
650 $aSimbiose
653 $aCarbon source use
653 $aSymbiotic efficiency
653 $aToleance to salinity and temperature
700 1 $aANDRAUS, M. de P.
700 1 $aBORBA, T. C. de O.
700 1 $aMARTIN-DIDONET, C. C. G.
700 1 $aFERREIRA, E. P. de B.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 48, n. 1, p. 43-50, jan./mar. 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
27/07/2009 |
|
Data da última atualização: |
20/05/2010 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
MEYER, J. D. F. SILVA, D. C. G. MORTEL, M. van de; PEDLEY, K. F. HILL, J. H. SHOEMAKER, R. C. ABDELNOOR, R. V. WHITHAM, S. A. GRAHAM, M. A. |
|
Afiliação: |
Ricardo Vilela Abdelnoor, CNPSo. |
|
Título: |
Identification and analyses of candidate Genes for Rpp4 mediated resistance to Asian Soybean rust in soybean (Glycine max). |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 17., 2009, San Diego. Final Abstract Guide. [S.l.], 2009. Guide. [S.l.], 2009. p. 47, W474. |
|
Idioma: |
Inglês |
|
Thesagro: |
Ferrugem. |
|
Thesaurus NAL: |
genes. |
|
Categoria do assunto: |
-- |
|
URL: |
https://intl.plantphysiol.org/cgi/reprint/150/1/295
|
|
Marc: |
LEADER 00691naa a2200145 a 4500
001 1471317
005 2010-05-20
008 2009 bl --- 0-- u #d
100 1 $aMEYER, J. D. F. SILVA, D. C. G. MORTEL, M. van de
245 $aIdentification and analyses of candidate Genes for Rpp4 mediated resistance to Asian Soybean rust in soybean (Glycine max).
260 $c2009
650 $agenes
650 $aFerrugem
700 1 $aPEDLEY, K. F. HILL, J. H. SHOEMAKER, R. C. ABDELNOOR, R. V. WHITHAM, S. A. GRAHAM, M. A.
773 $tIn: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 17., 2009, San Diego. Final Abstract Guide. [S.l.], 2009. Guide. [S.l.], 2009. p. 47, W474.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
