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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
27/10/2004 |
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Data da última atualização: |
04/11/2004 |
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Autoria: |
BORÉM, A.; OLHOFT, P. M.; LITTERER, L. A.; PLANK, D. W.; SOMERS, D. A. |
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Título: |
A plant binary vector with an antisense soybean UDP-glucose dehydrogenase gene. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 1, p. 49-52, Mar. 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean seeds are a valuable food and feed source because of their high protein and oil content. Conventional breeding has had limited success in increasing both oil and protein. Reducing the amount of cell wall polysaccharide in soybean seed could allow higher protein and oil content, thus increasing their economic value. UDP-glucose dehydrogenase is a key enzyme in cell wall polysaccharide synthesis. To investigate the role of UDP-glucose dehydrogenase in seed development, a transgenic approach was initiated. A new binary vector has been construted for soybean transformation that has the following features: i) the selectable marker hydromycin phosphotransferase (hpt); ii) placement of the selectable marker adjacent to the T-DNA left border and, iii) an antisense copy of the UDP-glucose dehydrogenase gene driven by the seed-specific vicilin promoter. This genetic construct was engineered to suppress the expression of UDP-glucose dehydrogenase in soybean seeds. |
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Palavras-Chave: |
Anti-senso; Antisense technology; Binary vectors; Genetic construct; Oil and protein content; Soybean; Soybean transformation; Vetor binário. |
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Thesagro: |
Gene; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01784naa a2200289 a 4500
001 1212655
005 2004-11-04
008 2003 bl uuuu u00u1 u #d
100 1 $aBORÉM, A.
245 $aA plant binary vector with an antisense soybean UDP-glucose dehydrogenase gene.
260 $c2003
520 $aSoybean seeds are a valuable food and feed source because of their high protein and oil content. Conventional breeding has had limited success in increasing both oil and protein. Reducing the amount of cell wall polysaccharide in soybean seed could allow higher protein and oil content, thus increasing their economic value. UDP-glucose dehydrogenase is a key enzyme in cell wall polysaccharide synthesis. To investigate the role of UDP-glucose dehydrogenase in seed development, a transgenic approach was initiated. A new binary vector has been construted for soybean transformation that has the following features: i) the selectable marker hydromycin phosphotransferase (hpt); ii) placement of the selectable marker adjacent to the T-DNA left border and, iii) an antisense copy of the UDP-glucose dehydrogenase gene driven by the seed-specific vicilin promoter. This genetic construct was engineered to suppress the expression of UDP-glucose dehydrogenase in soybean seeds.
650 $aGene
650 $aSoja
653 $aAnti-senso
653 $aAntisense technology
653 $aBinary vectors
653 $aGenetic construct
653 $aOil and protein content
653 $aSoybean
653 $aSoybean transformation
653 $aVetor binário
700 1 $aOLHOFT, P. M.
700 1 $aLITTERER, L. A.
700 1 $aPLANK, D. W.
700 1 $aSOMERS, D. A.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 3, n. 1, p. 49-52, Mar. 2003.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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