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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Mandioca e Fruticultura. |
Data corrente: |
28/02/1992 |
Data da última atualização: |
27/06/1995 |
Autoria: |
BONFANTE FASOLO, P.; VIAN, B.; PEROTTO, S.; FACCIO, A.; KNOX, J. P. |
Título: |
Cellulose and pectin localization in roots of mycorrhizal Allium porrum: labelling continuity between host cell wall and interfacial material. |
Ano de publicação: |
1990 |
Fonte/Imprenta: |
Planta, v.180, p.537-547, 1990. |
Idioma: |
Inglês |
Conteúdo: |
Two different types of contacts (or interfaces) exist between the plant host and the fungus during the vesicular-arbuscular mycorrhizal symbiosis, depending on whether the fungus is intercellular or intracellular. In the first case, the walls of the partners are in contact, while in the second case the fungal wall is separated from the host cytoplasm by the invaginated host plasmamembrana and by an interfacial material. In order to verify the origin of the interfacial material, affinity techniques which allow identification in situ of cell-wall components, were used. Cellobiohydrolase (CBH I) that binds to cellulose and a monoclonal antibody (JIM 5) that reacts with pectic components were tested on roots of Allium porrum L. (leek) colonized by Glomus versiforme (Karst.) Berch. Both probes gave a labelling specific for the host cell wall, but each probe labelled over specific and distinct areas. The CBH I -colloidal gold complex heavily labelled the thick epidermal cell walls, whereas JIM 5 only labelled this area weakly. Labelling of the hypodermis was mostly on intercellular material after treatment with JIM 5 and only on the wall when CBH I was used. Suberin bands found on the radial walls were never labelled. These components show a similar distribution in the interfacial material laid down around tehe vesicular-arbuscular mycorrhizal fungus indicating that the interfacial material is of host origin. |
Palavras-Chave: |
Allium - Cellulose - Cell Wall - Mycorrhiza (vesicular-arbuscular) - Pectin; Cell wall; Mycorrhiza; Pectin. |
Thesagro: |
Allium Porrum; Método; Micorriza; Microscopia Eletrônica; Polissacarídeo. |
Thesaurus Nal: |
Allium; cellulose. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02306naa a2200301 a 4500 001 1612787 005 1995-06-27 008 1990 bl --- 0-- u #d 100 1 $aBONFANTE FASOLO, P. 245 $aCellulose and pectin localization in roots of mycorrhizal Allium porrum$blabelling continuity between host cell wall and interfacial material. 260 $c1990 520 $aTwo different types of contacts (or interfaces) exist between the plant host and the fungus during the vesicular-arbuscular mycorrhizal symbiosis, depending on whether the fungus is intercellular or intracellular. In the first case, the walls of the partners are in contact, while in the second case the fungal wall is separated from the host cytoplasm by the invaginated host plasmamembrana and by an interfacial material. In order to verify the origin of the interfacial material, affinity techniques which allow identification in situ of cell-wall components, were used. Cellobiohydrolase (CBH I) that binds to cellulose and a monoclonal antibody (JIM 5) that reacts with pectic components were tested on roots of Allium porrum L. (leek) colonized by Glomus versiforme (Karst.) Berch. Both probes gave a labelling specific for the host cell wall, but each probe labelled over specific and distinct areas. The CBH I -colloidal gold complex heavily labelled the thick epidermal cell walls, whereas JIM 5 only labelled this area weakly. Labelling of the hypodermis was mostly on intercellular material after treatment with JIM 5 and only on the wall when CBH I was used. Suberin bands found on the radial walls were never labelled. These components show a similar distribution in the interfacial material laid down around tehe vesicular-arbuscular mycorrhizal fungus indicating that the interfacial material is of host origin. 650 $aAllium 650 $acellulose 650 $aAllium Porrum 650 $aMétodo 650 $aMicorriza 650 $aMicroscopia Eletrônica 650 $aPolissacarídeo 653 $aAllium - Cellulose - Cell Wall - Mycorrhiza (vesicular-arbuscular) - Pectin 653 $aCell wall 653 $aMycorrhiza 653 $aPectin 700 1 $aVIAN, B. 700 1 $aPEROTTO, S. 700 1 $aFACCIO, A. 700 1 $aKNOX, J. P. 773 $tPlanta$gv.180, p.537-547, 1990.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
07/04/2011 |
Data da última atualização: |
07/04/2011 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MADEIRO, A. S.; MORENZ, M. J. F.; PACIULLO, D. S. C.; LOPES, F. C. F. |
Afiliação: |
AFRANIO SILVA MADEIRO, UFRRJ; MIRTON JOSE FROTA MORENZ, CNPGL; DOMINGOS SAVIO CAMPOS PACIULLO, CNPGL; FERNANDO CESAR FERRAZ LOPES, CNPGL. |
Título: |
Avaliação de clones de capim-elefante de porte baixo sob pastejo de lotação rotativa. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Forum de Pós-Graduação da Universidade Federal Rural do Rio de Janeiro, 3., 2008, Seropédica. Anais... |
Idioma: |
Português |
Thesagro: |
Pastagem. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 00528naa a2200157 a 4500 001 1885374 005 2011-04-07 008 2008 bl --- 0-- u #d 100 1 $aMADEIRO, A. S. 245 $aAvaliação de clones de capim-elefante de porte baixo sob pastejo de lotação rotativa. 260 $c2008 650 $aPastagem 700 1 $aMORENZ, M. J. F. 700 1 $aPACIULLO, D. S. C. 700 1 $aLOPES, F. C. F. 773 $tForum de Pós-Graduação da Universidade Federal Rural do Rio de Janeiro, 3., 2008, Seropédica. Anais...
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