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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
07/12/2021 |
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Data da última atualização: |
07/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BONADIMANN, S. N. G. S.; CORDEIRO, M. D.; BAUTISTA, J. L. R.; SILVA, J. B. da; NICOLINO, R. R.; FONSECA, A. H. da; LOPES, L. B. |
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Afiliação: |
SUYANE NAYARA GARCIA SOCOLOSKI BONADIMANN, UFMT, Sinop-MT; MATHEUS DIAS CORDEIRO, UFRRJ, Seropedica-RJ; JOSÉ LUIS RODRÍGUEZ BAUTISTA, UFRRJ, Seropedica-RJ; JENEVALDO BARBOSA DA SILVA, UFVJM, Unaí-MG; RAFAEL ROMERO NICOLINO, UFVJM, Unaí-MG; ADIVALDO HENRIQUE DA FONSECA, UFRRJ, Seropedica-RJ; LUCIANO BASTOS LOPES, CPAMT. |
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Título: |
Serological and molecular detection of Theileria equi in horses from Sinop, Mato Grosso state, Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência Veterinária, v. 28, n. 3, p. 156-161, 2021. |
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ISSN: |
1413-0130 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Detecção sorológica e molecular de Theileria equi em equinos oriundos de Sinop, Mato Grosso, Brasil. |
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Conteúdo: |
Abstract: Equine piroplasmosis is the most important tick-borne disease to affect horses in Brazil. Theileria equi is one of the causative agents of equine piroplasmosis. Chronic cases are expected, in which the animals show no apparent signs of infection and remain asymptomatic but constitute a source of the infectious agent that ticks can spread. This study was conducted across 81 ranches located in the municipality of Sinop, State of Mato Grosso, Brazil. A sample calculation was performed to estimate the apparent prevalence of T. equi among horses. A total of 1,853 animals were included in the sampling analysis based on the information available from the Institute of Agricultural and Livestock Defense of Mato Grosso State. The serological analysis of 367 serum samples using an indirect enzyme-linked immunosorbent assay (ELISA) to detect anti-T. equi antibodies revealed that 337 animals were positive, representing a frequency of 90.70%. The molecular analysis to amplify the EMA-1 gene showed positivity in 20 of 89 tested samples. The fragments of four samples were sequenced and analyzed to determine their similarities to sequences from other species, based on sequences deposited at GenBank. All showed 100% similarity with T. equi. Our study represents the first report of T. equi antibodies among the equids in north-central region of Mato Grosso, revealing the widespread distribution of seropositive animals. | Resumo: A piroplasmose equina é a doença transmitida por carrapatos mais importante em cavalos no Brasil. Theileria equi é um dos agentes causadores da piroplasmose equina. São esperados casos crônicos, nos quais os animais não apresentam sinais aparentes de infecção e permanecem assintomáticos, mas constituem uma fonte de infecção e disseminação por carrapatos. Este estudo foi realizado em 81 fazendas localizadas no município de Sinop, Estado de Mato Grosso, Brasil. Um cálculo amostral foi realizado para estimar a prevalência aparente de T. equi entre cavalos. No total, 1.853 animais foram incluídos na análise amostral com base nas informações disponíveis no Instituto de Defesa Agropecuária do Estado de Mato Grosso. A análise sorológica de 367 amostras de soro por meio de ensaio imunoenzimático indireto (ELISA) para detecção de anticorpos anti-T. equi revelou que 337 animais eram positivos, representando uma frequência de 90,70%. A análise molecular para o gene EMA-1 mostrou positividade em 20 das 89 amostras testadas. Os fragmentos de quatro amostras foram sequenciados e analisados para determinar suas semelhanças com sequências de outras espécies, a partir das sequências depositadas no GenBank. Todos mostraram 100% de similaridade com T. equi. Nosso estudo representa o primeiro relato de anticorpos contra T. equi entre os equídeos na região centro norte de Mato Grosso, revelando a ampla distribuição de animais soropositivos. MenosAbstract: Equine piroplasmosis is the most important tick-borne disease to affect horses in Brazil. Theileria equi is one of the causative agents of equine piroplasmosis. Chronic cases are expected, in which the animals show no apparent signs of infection and remain asymptomatic but constitute a source of the infectious agent that ticks can spread. This study was conducted across 81 ranches located in the municipality of Sinop, State of Mato Grosso, Brazil. A sample calculation was performed to estimate the apparent prevalence of T. equi among horses. A total of 1,853 animals were included in the sampling analysis based on the information available from the Institute of Agricultural and Livestock Defense of Mato Grosso State. The serological analysis of 367 serum samples using an indirect enzyme-linked immunosorbent assay (ELISA) to detect anti-T. equi antibodies revealed that 337 animals were positive, representing a frequency of 90.70%. The molecular analysis to amplify the EMA-1 gene showed positivity in 20 of 89 tested samples. The fragments of four samples were sequenced and analyzed to determine their similarities to sequences from other species, based on sequences deposited at GenBank. All showed 100% similarity with T. equi. Our study represents the first report of T. equi antibodies among the equids in north-central region of Mato Grosso, revealing the widespread distribution of seropositive animals. | Resumo: A piroplasmose equina é a doença transmitida por carrapa... Mostrar Tudo |
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Palavras-Chave: |
Mato Grosso; Sinop-MT; Sorologia. |
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Thesagro: |
Cavalo; Elisa; Eqüino; Piroplasmose. |
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Thesaurus Nal: |
Equine piroplasmosis; Serology; Theileria; Theileria equi. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228658/1/2021-cpamt-lbl-serological-molecular-detection-theileria-horses-sinop-mt.pdf
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Marc: |
LEADER 04016naa a2200349 a 4500
001 2137272
005 2021-12-07
008 2021 bl uuuu u00u1 u #d
022 $a1413-0130
100 1 $aBONADIMANN, S. N. G. S.
245 $aSerological and molecular detection of Theileria equi in horses from Sinop, Mato Grosso state, Brazil.$h[electronic resource]
260 $c2021
500 $aTítulo em português: Detecção sorológica e molecular de Theileria equi em equinos oriundos de Sinop, Mato Grosso, Brasil.
520 $aAbstract: Equine piroplasmosis is the most important tick-borne disease to affect horses in Brazil. Theileria equi is one of the causative agents of equine piroplasmosis. Chronic cases are expected, in which the animals show no apparent signs of infection and remain asymptomatic but constitute a source of the infectious agent that ticks can spread. This study was conducted across 81 ranches located in the municipality of Sinop, State of Mato Grosso, Brazil. A sample calculation was performed to estimate the apparent prevalence of T. equi among horses. A total of 1,853 animals were included in the sampling analysis based on the information available from the Institute of Agricultural and Livestock Defense of Mato Grosso State. The serological analysis of 367 serum samples using an indirect enzyme-linked immunosorbent assay (ELISA) to detect anti-T. equi antibodies revealed that 337 animals were positive, representing a frequency of 90.70%. The molecular analysis to amplify the EMA-1 gene showed positivity in 20 of 89 tested samples. The fragments of four samples were sequenced and analyzed to determine their similarities to sequences from other species, based on sequences deposited at GenBank. All showed 100% similarity with T. equi. Our study represents the first report of T. equi antibodies among the equids in north-central region of Mato Grosso, revealing the widespread distribution of seropositive animals. | Resumo: A piroplasmose equina é a doença transmitida por carrapatos mais importante em cavalos no Brasil. Theileria equi é um dos agentes causadores da piroplasmose equina. São esperados casos crônicos, nos quais os animais não apresentam sinais aparentes de infecção e permanecem assintomáticos, mas constituem uma fonte de infecção e disseminação por carrapatos. Este estudo foi realizado em 81 fazendas localizadas no município de Sinop, Estado de Mato Grosso, Brasil. Um cálculo amostral foi realizado para estimar a prevalência aparente de T. equi entre cavalos. No total, 1.853 animais foram incluídos na análise amostral com base nas informações disponíveis no Instituto de Defesa Agropecuária do Estado de Mato Grosso. A análise sorológica de 367 amostras de soro por meio de ensaio imunoenzimático indireto (ELISA) para detecção de anticorpos anti-T. equi revelou que 337 animais eram positivos, representando uma frequência de 90,70%. A análise molecular para o gene EMA-1 mostrou positividade em 20 das 89 amostras testadas. Os fragmentos de quatro amostras foram sequenciados e analisados para determinar suas semelhanças com sequências de outras espécies, a partir das sequências depositadas no GenBank. Todos mostraram 100% de similaridade com T. equi. Nosso estudo representa o primeiro relato de anticorpos contra T. equi entre os equídeos na região centro norte de Mato Grosso, revelando a ampla distribuição de animais soropositivos.
650 $aEquine piroplasmosis
650 $aSerology
650 $aTheileria
650 $aTheileria equi
650 $aCavalo
650 $aElisa
650 $aEqüino
650 $aPiroplasmose
653 $aMato Grosso
653 $aSinop-MT
653 $aSorologia
700 1 $aCORDEIRO, M. D.
700 1 $aBAUTISTA, J. L. R.
700 1 $aSILVA, J. B. da
700 1 $aNICOLINO, R. R.
700 1 $aFONSECA, A. H. da
700 1 $aLOPES, L. B.
773 $tRevista Brasileira de Ciência Veterinária$gv. 28, n. 3, p. 156-161, 2021.
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Embrapa Agrossilvipastoril (CPAMT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
12/04/2022 |
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Data da última atualização: |
09/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SANTOS, R. T. dos S. e; TORRES, L. H. P. S.; BIASOTO, A. C. T.; FREITAS, S. T. de; MELO, N. F. de; SILVA, F. L. H. da. |
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Afiliação: |
RENATA TORRES DOS SANTOS E SANTOS, UFPB; LUIS HENRIQUE PEREIRA SÁ TORRES, UNEB; ALINE TELLES BIASOTO MARQUES, CPATSA; SERGIO TONETTO DE FREITAS, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA; FLÁVIO LUIZ HONORATO DA SILVA, UFPB. |
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Título: |
Optimization of pectinolytic hydrolysis in Caatinga passion fruit wine must with commercial pectinase, according to the central composite rotatable design approach. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Research, Society and Development, v. 11, n. 5, e14511528088, 2022. |
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ISSN: |
2525-3409 |
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DOI: |
http://dx.doi.org/10.33448/rsd-v11i5.2808 |
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Idioma: |
Inglês |
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Conteúdo: |
The Passiflora cincinnata Mast. has high flavor and potential for wine and other alcoholic beverages production. However, for wine production, the presence of pectin becomes undesirable, as it influences the efficiency of the fermentation process and the clarity of the final product. The objective of this study was to optimize the pectin hydrolysis process in Caatinga passion fruit must for wine production with pectinolytic enzymes, using a Central Composite Rotatable Design (CCRD). The 2³ factorial CCRD, E01 to E17 assays, was applied with six axial tests and three replications in the central point. Caatinga passion fruit must was obtained with pulp and distilled water (40:60 proporcion). Commercial enzyme with high concentration of pectinase was tested. Concentration of enzyme (0.014- 0.056 g L-1), working temperature (43-57°C) and reaction time (12-139min) were the independent variables in the optimization process; and the soluble pectin content (mg 100g-1) corresponded to a process-dependent variable. Prior to the beginning of the assays, in order to provide the optimum working pH range for the pectinase, correction of the must pH to 3.9. The initial Caatinga passion fruit wine must had 24.06 mg 100g-1 of soluble pectin in its composition. After the optimization using CCRD, the treatments presented pectin contents ranging from 6.81 (E16: 0.014g L-1/50°C/75min) to 0.00 mg 100 g-1 (E05: 0.05 g L-1/45°C/ 30min and E06: 0.05 g L-1/45°C/120min). According to the results, to a satisfactory process of pectin hydrolysis in Caatinga passion fruit wine must be added 0.05 g L-1 of pectinase at 45°C for 30min. MenosThe Passiflora cincinnata Mast. has high flavor and potential for wine and other alcoholic beverages production. However, for wine production, the presence of pectin becomes undesirable, as it influences the efficiency of the fermentation process and the clarity of the final product. The objective of this study was to optimize the pectin hydrolysis process in Caatinga passion fruit must for wine production with pectinolytic enzymes, using a Central Composite Rotatable Design (CCRD). The 2³ factorial CCRD, E01 to E17 assays, was applied with six axial tests and three replications in the central point. Caatinga passion fruit must was obtained with pulp and distilled water (40:60 proporcion). Commercial enzyme with high concentration of pectinase was tested. Concentration of enzyme (0.014- 0.056 g L-1), working temperature (43-57°C) and reaction time (12-139min) were the independent variables in the optimization process; and the soluble pectin content (mg 100g-1) corresponded to a process-dependent variable. Prior to the beginning of the assays, in order to provide the optimum working pH range for the pectinase, correction of the must pH to 3.9. The initial Caatinga passion fruit wine must had 24.06 mg 100g-1 of soluble pectin in its composition. After the optimization using CCRD, the treatments presented pectin contents ranging from 6.81 (E16: 0.014g L-1/50°C/75min) to 0.00 mg 100 g-1 (E05: 0.05 g L-1/45°C/ 30min and E06: 0.05 g L-1/45°C/120min). According to the results, to a... Mostrar Tudo |
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Palavras-Chave: |
Bioma Caatinga; Caatinga biome; Delineamento Composto Central Rotaciona; Design fatorial; DOE; Maracuja do mato; Passiflora cincinnata. |
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Thesagro: |
Caatinga; Maracujá; Mosto; Pectina. |
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Thesaurus NAL: |
Passion fruits. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02750naa a2200349 a 4500
001 2142152
005 2022-09-09
008 2022 bl uuuu u00u1 u #d
022 $a2525-3409
024 7 $ahttp://dx.doi.org/10.33448/rsd-v11i5.2808$2DOI
100 1 $aSANTOS, R. T. dos S. e
245 $aOptimization of pectinolytic hydrolysis in Caatinga passion fruit wine must with commercial pectinase, according to the central composite rotatable design approach.$h[electronic resource]
260 $c2022
520 $aThe Passiflora cincinnata Mast. has high flavor and potential for wine and other alcoholic beverages production. However, for wine production, the presence of pectin becomes undesirable, as it influences the efficiency of the fermentation process and the clarity of the final product. The objective of this study was to optimize the pectin hydrolysis process in Caatinga passion fruit must for wine production with pectinolytic enzymes, using a Central Composite Rotatable Design (CCRD). The 2³ factorial CCRD, E01 to E17 assays, was applied with six axial tests and three replications in the central point. Caatinga passion fruit must was obtained with pulp and distilled water (40:60 proporcion). Commercial enzyme with high concentration of pectinase was tested. Concentration of enzyme (0.014- 0.056 g L-1), working temperature (43-57°C) and reaction time (12-139min) were the independent variables in the optimization process; and the soluble pectin content (mg 100g-1) corresponded to a process-dependent variable. Prior to the beginning of the assays, in order to provide the optimum working pH range for the pectinase, correction of the must pH to 3.9. The initial Caatinga passion fruit wine must had 24.06 mg 100g-1 of soluble pectin in its composition. After the optimization using CCRD, the treatments presented pectin contents ranging from 6.81 (E16: 0.014g L-1/50°C/75min) to 0.00 mg 100 g-1 (E05: 0.05 g L-1/45°C/ 30min and E06: 0.05 g L-1/45°C/120min). According to the results, to a satisfactory process of pectin hydrolysis in Caatinga passion fruit wine must be added 0.05 g L-1 of pectinase at 45°C for 30min.
650 $aPassion fruits
650 $aCaatinga
650 $aMaracujá
650 $aMosto
650 $aPectina
653 $aBioma Caatinga
653 $aCaatinga biome
653 $aDelineamento Composto Central Rotaciona
653 $aDesign fatorial
653 $aDOE
653 $aMaracuja do mato
653 $aPassiflora cincinnata
700 1 $aTORRES, L. H. P. S.
700 1 $aBIASOTO, A. C. T.
700 1 $aFREITAS, S. T. de
700 1 $aMELO, N. F. de
700 1 $aSILVA, F. L. H. da
773 $tResearch, Society and Development$gv. 11, n. 5, e14511528088, 2022.
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