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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/04/2005 |
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Data da última atualização: |
03/10/2016 |
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Autoria: |
FAHEY, J.; BOLAND, M. P.; O'CALLAGHAN, D. |
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Título: |
The effects of dietary urea on embryo development in superovulated donor ewes and on early embryo survival and development in recipient ewes. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Animal Science, v. 72, pt 2 , p. 395-400, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim was to examine the effect of dietary urea on yield and quality of embryos in superovulated donor ewes, and on embryo survival in recipient ewes. Ewes (25 donors and 34 recipients) were offered 1.25 kg of grass meal containing 50 g urea per ewe per day on a group basis; the remaining ewes (30 donors and 32 recipients) received 1.25 kg untreated grass meal per day. After 5 days, ewes were synchronized using a progestagen pessary for 14 days and stimulated with pregnant male serum gonadotropin. Donors were naturally mated; recipients were detected in oestrus using vasectomized rams. On day 4 following the onset of oestrus, embryos were recovered from donors, graded morphologically and cell number counted. Two embryos of acceptable morphological grade (= eight cells on day 4) were then surgically transferred to recipient ewes. Recipients were slaughtered between days 34 and 36 post oestrus. The mean number of embryos recovered per ewe [3.68 (s.e.O-41) v. 3.83 (s.e. 0-45)J was not different between untreated and urea-treated ewes, respectlvely. However, urea treatment of donor ewes reduced the mean cell number per embryo (10.27 (s.e.0.27) v. 8.17 (s.e. 0.29) for untreated and urea-treated danar ewes, respectively, P < 0.001). Similarly, the percentage of embryos recovered that had =c10 cells and their mean cell number WtlS reduced following urea treatment of donor ewes (82/113 (73%) v. 39/93 (42%), P < 0.01 and 11.61 (s.e.0.20) v. 10-41 (s.e.0.29), P < 0.001) for untreated and urea-treated ewes, respectively. The diet offered to recipient ewes had no effect on lhe number of ewes confirmed pregnant (24/32 (75%) v. 28/34 (82%) or on embryo survival following transfer (45/64 (70%) v. 51/68 (75%) for untreated and urea-treated ewes, respectively, P> 0.05). Excess non- protein nitrogen in lhe form of dietary urea reduced embryo quality. However, embryo survival in recipient ewes was not affected when good quality embryos were transferred. This suggests that the deleterious effects of excess dietary urea are due to alterations to the oocyte and (or) the oviductal environment rather than changes in the uterine environment. MenosThe aim was to examine the effect of dietary urea on yield and quality of embryos in superovulated donor ewes, and on embryo survival in recipient ewes. Ewes (25 donors and 34 recipients) were offered 1.25 kg of grass meal containing 50 g urea per ewe per day on a group basis; the remaining ewes (30 donors and 32 recipients) received 1.25 kg untreated grass meal per day. After 5 days, ewes were synchronized using a progestagen pessary for 14 days and stimulated with pregnant male serum gonadotropin. Donors were naturally mated; recipients were detected in oestrus using vasectomized rams. On day 4 following the onset of oestrus, embryos were recovered from donors, graded morphologically and cell number counted. Two embryos of acceptable morphological grade (= eight cells on day 4) were then surgically transferred to recipient ewes. Recipients were slaughtered between days 34 and 36 post oestrus. The mean number of embryos recovered per ewe [3.68 (s.e.O-41) v. 3.83 (s.e. 0-45)J was not different between untreated and urea-treated ewes, respectlvely. However, urea treatment of donor ewes reduced the mean cell number per embryo (10.27 (s.e.0.27) v. 8.17 (s.e. 0.29) for untreated and urea-treated danar ewes, respectively, P < 0.001). Similarly, the percentage of embryos recovered that had =c10 cells and their mean cell number WtlS reduced following urea treatment of donor ewes (82/113 (73%) v. 39/93 (42%), P < 0.01 and 11.61 (s.e.0.20) v. 10-41 (s.e.0.29), P < 0.001) for untreate... Mostrar Tudo |
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Thesagro: |
Cordeiro; Embrião; Ovino; Superovulação. |
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Thesaurus Nal: |
urea. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02759naa a2200205 a 4500
001 1531084
005 2016-10-03
008 2001 bl uuuu u00u1 u #d
100 1 $aFAHEY, J.
245 $aThe effects of dietary urea on embryo development in superovulated donor ewes and on early embryo survival and development in recipient ewes.
260 $c2001
520 $aThe aim was to examine the effect of dietary urea on yield and quality of embryos in superovulated donor ewes, and on embryo survival in recipient ewes. Ewes (25 donors and 34 recipients) were offered 1.25 kg of grass meal containing 50 g urea per ewe per day on a group basis; the remaining ewes (30 donors and 32 recipients) received 1.25 kg untreated grass meal per day. After 5 days, ewes were synchronized using a progestagen pessary for 14 days and stimulated with pregnant male serum gonadotropin. Donors were naturally mated; recipients were detected in oestrus using vasectomized rams. On day 4 following the onset of oestrus, embryos were recovered from donors, graded morphologically and cell number counted. Two embryos of acceptable morphological grade (= eight cells on day 4) were then surgically transferred to recipient ewes. Recipients were slaughtered between days 34 and 36 post oestrus. The mean number of embryos recovered per ewe [3.68 (s.e.O-41) v. 3.83 (s.e. 0-45)J was not different between untreated and urea-treated ewes, respectlvely. However, urea treatment of donor ewes reduced the mean cell number per embryo (10.27 (s.e.0.27) v. 8.17 (s.e. 0.29) for untreated and urea-treated danar ewes, respectively, P < 0.001). Similarly, the percentage of embryos recovered that had =c10 cells and their mean cell number WtlS reduced following urea treatment of donor ewes (82/113 (73%) v. 39/93 (42%), P < 0.01 and 11.61 (s.e.0.20) v. 10-41 (s.e.0.29), P < 0.001) for untreated and urea-treated ewes, respectively. The diet offered to recipient ewes had no effect on lhe number of ewes confirmed pregnant (24/32 (75%) v. 28/34 (82%) or on embryo survival following transfer (45/64 (70%) v. 51/68 (75%) for untreated and urea-treated ewes, respectively, P> 0.05). Excess non- protein nitrogen in lhe form of dietary urea reduced embryo quality. However, embryo survival in recipient ewes was not affected when good quality embryos were transferred. This suggests that the deleterious effects of excess dietary urea are due to alterations to the oocyte and (or) the oviductal environment rather than changes in the uterine environment.
650 $aurea
650 $aCordeiro
650 $aEmbrião
650 $aOvino
650 $aSuperovulação
700 1 $aBOLAND, M. P.
700 1 $aO'CALLAGHAN, D.
773 $tAnimal Science$gv. 72, pt 2 , p. 395-400, 2001.
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Embrapa Caprinos e Ovinos (CNPC) |
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