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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
15/08/2022 |
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Data da última atualização: |
15/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CABRAL, C. S.; FONSECA, M. E. N.; BOITEUX, L. S.; BARBOZA, E. A.; VELOSO, J. S.; LOURENÇO JUNIOR, V.; REIS, A. |
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Afiliação: |
C. S. CABRAL, UNIVERSIDADE DE BRASÍLIA; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; LEONARDO SILVA BOITEUX, CNPH; E. A. BARBOZA, UNIVERSIDADE DE BRASÍLIA; J. S. VELOSO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; VALDIR LOURENCO JUNIOR, CNPH; AILTON REIS, CNPH. |
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Título: |
Phenotypic and genetic variability of fungal isolates associated with the Septoria leaf spot disease of lettuce (Lactuca sativa) in Brazil. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Plant Diseases and Protection, v. 129, n. 4, p. 53-62, Aug. 2022. |
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DOI: |
https://doi.org/10.1007/s41348-021-00537-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Septoria leaf spot is a severe disease of lettuce in tropical and subtropical regions, inducing yield/quality losses and increasing production costs. Globally, Septoria lactucae has been reported as the major pathogen associated with this disease. However, a complex of Septoria species has been also reported in association with lettuce crop around the world. Extensive surveys of Septoria species associated with this disease have not been conducted in the Neotropics. Therefore, the objective of the present study was to undergo a phenotypic and genetic characterization of a collection of 32 Brazilian Septoria isolates causing lettuce leaf spot. Morphometrical characterization of conidia and pycnidia was conducted with ten isolates obtained from three lettuce-producing regions in Brazil. In addition, partial sequences of four gene regions (β-tubulin, RPB2, actin, and calmodulin) were used in phylogenetic analyses of all 32 Septoria isolates. All tested isolates were pathogenic to two lettuce cultivars and morphometrically similar to each other. These isolates grouped together into a single monophyletic clade composed exclusively by genuine S. lactucae isolates. Therefore, S. lactucae was found to be the sole species associated with the Septoria leaf spot of lettuce in Brazil. The number of polymorphic sites (n = 27), mutations (n = 190), the level of nucleotide diversity (0.00262), and the average number of nucleotide differences (2.961) were relatively low. Notwithstanding, this genetic variability allowed the identification of 17 haplotypes amongst the 32 Brazilian isolates. This information will help to guide resistance breeding programs and establish more effective management strategies of this disease. MenosSeptoria leaf spot is a severe disease of lettuce in tropical and subtropical regions, inducing yield/quality losses and increasing production costs. Globally, Septoria lactucae has been reported as the major pathogen associated with this disease. However, a complex of Septoria species has been also reported in association with lettuce crop around the world. Extensive surveys of Septoria species associated with this disease have not been conducted in the Neotropics. Therefore, the objective of the present study was to undergo a phenotypic and genetic characterization of a collection of 32 Brazilian Septoria isolates causing lettuce leaf spot. Morphometrical characterization of conidia and pycnidia was conducted with ten isolates obtained from three lettuce-producing regions in Brazil. In addition, partial sequences of four gene regions (β-tubulin, RPB2, actin, and calmodulin) were used in phylogenetic analyses of all 32 Septoria isolates. All tested isolates were pathogenic to two lettuce cultivars and morphometrically similar to each other. These isolates grouped together into a single monophyletic clade composed exclusively by genuine S. lactucae isolates. Therefore, S. lactucae was found to be the sole species associated with the Septoria leaf spot of lettuce in Brazil. The number of polymorphic sites (n = 27), mutations (n = 190), the level of nucleotide diversity (0.00262), and the average number of nucleotide differences (2.961) were relatively low. Notwithstandin... Mostrar Tudo |
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Palavras-Chave: |
Phylogenetic analysis; Septoria leaf spot. |
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Thesagro: |
Alface; Lactuca Sativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145442/1/Cabral-et-al-2022-Journal-of-Plant-Diseasees-and-Protection-129-p-53-62-2022.pdf
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Marc: |
LEADER 02579naa a2200253 a 4500
001 2145442
005 2022-08-15
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s41348-021-00537-9$2DOI
100 1 $aCABRAL, C. S.
245 $aPhenotypic and genetic variability of fungal isolates associated with the Septoria leaf spot disease of lettuce (Lactuca sativa) in Brazil.$h[electronic resource]
260 $c2022
520 $aSeptoria leaf spot is a severe disease of lettuce in tropical and subtropical regions, inducing yield/quality losses and increasing production costs. Globally, Septoria lactucae has been reported as the major pathogen associated with this disease. However, a complex of Septoria species has been also reported in association with lettuce crop around the world. Extensive surveys of Septoria species associated with this disease have not been conducted in the Neotropics. Therefore, the objective of the present study was to undergo a phenotypic and genetic characterization of a collection of 32 Brazilian Septoria isolates causing lettuce leaf spot. Morphometrical characterization of conidia and pycnidia was conducted with ten isolates obtained from three lettuce-producing regions in Brazil. In addition, partial sequences of four gene regions (β-tubulin, RPB2, actin, and calmodulin) were used in phylogenetic analyses of all 32 Septoria isolates. All tested isolates were pathogenic to two lettuce cultivars and morphometrically similar to each other. These isolates grouped together into a single monophyletic clade composed exclusively by genuine S. lactucae isolates. Therefore, S. lactucae was found to be the sole species associated with the Septoria leaf spot of lettuce in Brazil. The number of polymorphic sites (n = 27), mutations (n = 190), the level of nucleotide diversity (0.00262), and the average number of nucleotide differences (2.961) were relatively low. Notwithstanding, this genetic variability allowed the identification of 17 haplotypes amongst the 32 Brazilian isolates. This information will help to guide resistance breeding programs and establish more effective management strategies of this disease.
650 $aAlface
650 $aLactuca Sativa
653 $aPhylogenetic analysis
653 $aSeptoria leaf spot
700 1 $aFONSECA, M. E. N.
700 1 $aBOITEUX, L. S.
700 1 $aBARBOZA, E. A.
700 1 $aVELOSO, J. S.
700 1 $aLOURENÇO JUNIOR, V.
700 1 $aREIS, A.
773 $tJournal of Plant Diseases and Protection$gv. 129, n. 4, p. 53-62, Aug. 2022.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 797 | |
| 10. |  | REIS, A.; BOITEUX, L. S. Caracterização de raças de Verticillium de isolados obtidos de diferentes hospedeira e Estados do Brasil. Tropical Plant Pathology, Brasília, DF, v. 33, p. S226, ago. 2008. Suplemento. Resumo MIC 047. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual meeting of the Brazilian Phytophatological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 18. | | REIS, A.; BOITEUX, L. S. Círculo de plantas hospedeiras de isolados de Stemphylium solani. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. s31, ago. 2007. Suplemento. Resumo 158. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 797 | |
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