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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIANA CORTES BOITE, FIOCRUZ; SABINE WOHLRES-VIANA, UFJF; GUSTAVO BRUNO MOTA, UNB; RAQUEL VARELA SERAPIÃO, PESAGRO; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF. |
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Título: |
Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Cryobiology, v. 63, n. 3, p. 256-262, 2011. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2011.09.135 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. MenosThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-... Mostrar Tudo |
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Palavras-Chave: |
Embryo. |
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Thesaurus Nal: |
cryopreservation; osmosis; vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02924naa a2200265 a 4500
001 1910654
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2011.09.135$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aOsmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro.$h[electronic resource]
260 $c2011
520 $aThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system.
650 $acryopreservation
650 $aosmosis
650 $avitrification
653 $aEmbryo
700 1 $aBOITE, M. C.
700 1 $aWOHLRES-VIANA, S.
700 1 $aMOTA, G. B.
700 1 $aSERAPIÃO, R. V.
700 1 $aSÁ, W. F. de
700 1 $aVIANA, J. H. M.
700 1 $aNOGUEIRA, L. A. G.
773 $tCryobiology$gv. 63, n. 3, p. 256-262, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
19/02/2010 |
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Data da última atualização: |
26/04/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
FERREIRA, M. das G. R.; SANTOS, M. R. A. dos; BRAGADO, A. C. R. |
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Afiliação: |
Maria das Graças Rodrigues Ferreira, Embrapa Rondônia; Maurício Reginaldo Alves dos Santos, Embrapa Rondônia; Ana Cleide Ribeiro Bragado Graduanda FARO. |
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Título: |
Propagação in vitro de cupuaçuzeiro: desinfestação de explantes florais. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Separata de: Saber Científico, Porto Velho, v. 2, n. 2, p. 37 - 44, jul./dez.,2009. |
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Idioma: |
Português |
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Conteúdo: |
O trabalho teve como objetivo desenvolver um protocolo para a desinfestação de explantes florais de cupuaçuzeiro, visando ao seu estabelecimento in vitro. Botões florais fechados, oriundos de cupuaçuzeiros sem sementes, foram lavados com água destilada e imersos em álcool 70% (v/v) por um minuto. Em câmara de fluxo, os botões foram imersos em hipoclorito de sódio a 0,25 e 0,50%, durante 20 e 30 minutos, e lavados três vezes com água estéril. Os botões foram segmentados em pétala, estaminóide, lígula e ovário, os quais foram inoculados em meio MS contendo ágar (8 g.L-1), com e sem cefotaxima (100 mg.L-1). Avaliou-se a contaminação dos explantes nos 20 dias subseqüentes e constatou-se que a utilização de antibiótico no meio de cultura foi essencial para o controle da contaminação. A fim de reduzir a oxidação dos tecidos, devido ao maior tempo de exposição ao hipoclorito de sódio, recomenda-se a concentração de 0,25% e 20 minutos de imersão dos explantes. |
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Palavras-Chave: |
Cefotaxima; Cultivo in vitro; Hipoclorito de sódio. |
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Thesaurus NAL: |
cefotaxime; in vitro culture; sodium hypochlorite. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-RO-2010/14403/1/propagacao-invitro.pdf
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Marc: |
LEADER 01660naa a2200217 a 4500
001 1710816
005 2010-04-26
008 2009 bl uuuu u00u1 u #d
100 1 $aFERREIRA, M. das G. R.
245 $aPropagação in vitro de cupuaçuzeiro$bdesinfestação de explantes florais.
260 $c2009
520 $aO trabalho teve como objetivo desenvolver um protocolo para a desinfestação de explantes florais de cupuaçuzeiro, visando ao seu estabelecimento in vitro. Botões florais fechados, oriundos de cupuaçuzeiros sem sementes, foram lavados com água destilada e imersos em álcool 70% (v/v) por um minuto. Em câmara de fluxo, os botões foram imersos em hipoclorito de sódio a 0,25 e 0,50%, durante 20 e 30 minutos, e lavados três vezes com água estéril. Os botões foram segmentados em pétala, estaminóide, lígula e ovário, os quais foram inoculados em meio MS contendo ágar (8 g.L-1), com e sem cefotaxima (100 mg.L-1). Avaliou-se a contaminação dos explantes nos 20 dias subseqüentes e constatou-se que a utilização de antibiótico no meio de cultura foi essencial para o controle da contaminação. A fim de reduzir a oxidação dos tecidos, devido ao maior tempo de exposição ao hipoclorito de sódio, recomenda-se a concentração de 0,25% e 20 minutos de imersão dos explantes.
650 $acefotaxime
650 $ain vitro culture
650 $asodium hypochlorite
653 $aCefotaxima
653 $aCultivo in vitro
653 $aHipoclorito de sódio
700 1 $aSANTOS, M. R. A. dos
700 1 $aBRAGADO, A. C. R.
773 $tSeparata de: Saber Científico, Porto Velho$gv. 2, n. 2, p. 37 - 44, jul./dez.,2009.
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Embrapa Rondônia (CPAF-RO) |
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