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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/08/1995 |
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Data da última atualização: |
31/08/1995 |
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Autoria: |
BOHER, B.; KPEMOUA, K.; NICOLE, M.; LUISETTI, J.; GEIGER, J. P. |
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Afiliação: |
ORSTOM, Laboratoire de Phytopathologie, B.P. 5045, 34032 Montpellier, France; fourty author:INRA, Station de Pathologie Vegetale et Phytobacteriologie, B.P. 57, 49071 Beaucouze Cedex, France. |
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Título: |
Ultrastructure of Interactions between cassava and xanthomonas campestris pv. manihotis: Cytochemistry of cellulose and pectin degradation in a susceptible cultivar. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Phytopathology, v.85, n.7, p.777-788, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A cytochemical investigation was carried out on the development of an aggressive strain of Xanthomonas campestris pv. manihotis, responsible for the cassava bacterial blight, to gain better insights into molecular and cellular mechanisms involved in leaf cell wall degradation by this pathogen. The use of anti-pectin monoclonal antibodies revealed that the plant middle lamellae were damaged during the infection process, from the epiphytic stage on the leaf surface to invasion of vascular bundles. In parallel, application of a Beta-1,4-exoglucanase-gold probe to healthy and infected tissues indicated that primary and secondary cell walls were also altered. Quantitation of gold labeling confirmed that pectin was more severely degraded than cellulose. Accumulation of pectinlike compounds was also detected in occluded infected vessels. Bacterial-surrounding sheaths, which were coutinely seen during pathogenesis early after leaf inoculation, had a dense of loosened fibrillar appearance and were differentiated from the pathogen cell wall. Close association occurred between extracellular fibrils and leaf cell both at early and advanced stages of wall degradation. Bacterial extracellular sheaths were often seen deep in host cell walls, sometimes enclosing residual plant cell wall fragments. Our cytochemical data demonstrated that cell wall degradation of cassava by Xanthomonas campestris pv. manihotis plays an important role in host tissue colonization. |
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Palavras-Chave: |
Manihot esculenta crantz; Plant material; Tissue processing. |
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Thesaurus Nal: |
cytochemistry. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02148naa a2200217 a 4500
001 1634002
005 1995-08-31
008 1995 bl uuuu u00u1 u #d
100 1 $aBOHER, B.
245 $aUltrastructure of Interactions between cassava and xanthomonas campestris pv. manihotis$bCytochemistry of cellulose and pectin degradation in a susceptible cultivar.
260 $c1995
520 $aA cytochemical investigation was carried out on the development of an aggressive strain of Xanthomonas campestris pv. manihotis, responsible for the cassava bacterial blight, to gain better insights into molecular and cellular mechanisms involved in leaf cell wall degradation by this pathogen. The use of anti-pectin monoclonal antibodies revealed that the plant middle lamellae were damaged during the infection process, from the epiphytic stage on the leaf surface to invasion of vascular bundles. In parallel, application of a Beta-1,4-exoglucanase-gold probe to healthy and infected tissues indicated that primary and secondary cell walls were also altered. Quantitation of gold labeling confirmed that pectin was more severely degraded than cellulose. Accumulation of pectinlike compounds was also detected in occluded infected vessels. Bacterial-surrounding sheaths, which were coutinely seen during pathogenesis early after leaf inoculation, had a dense of loosened fibrillar appearance and were differentiated from the pathogen cell wall. Close association occurred between extracellular fibrils and leaf cell both at early and advanced stages of wall degradation. Bacterial extracellular sheaths were often seen deep in host cell walls, sometimes enclosing residual plant cell wall fragments. Our cytochemical data demonstrated that cell wall degradation of cassava by Xanthomonas campestris pv. manihotis plays an important role in host tissue colonization.
650 $acytochemistry
653 $aManihot esculenta crantz
653 $aPlant material
653 $aTissue processing
700 1 $aKPEMOUA, K.
700 1 $aNICOLE, M.
700 1 $aLUISETTI, J.
700 1 $aGEIGER, J. P.
773 $tPhytopathology$gv.85, n.7, p.777-788, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 15 | |
| 4. |  | COSTA, S. B.; LOPES, C. A.; BOHER, B.; FERREIRA, M. Diversidade bioquímica e patogênica de isolados de Ralstonia solanacearum obtidos de diferentes hospedeiras da Região Amazônica. Horticultura Brasileira, Brasília, DF, v. 19, n. 2, jul. 2001. não paginado. 1 CD-ROM. Trabalho apresentado no 41° Congresso Brasileiro de Olericultura, 2001.| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | COSTA, S. B.; LOPES, C. A.; BOHER, B.; FERREIRA, M. Virulência de isolados de Ralstonia solanacearum ao tomateiro. Fitopatologia Brasileira, Brasília, DF, v. 26, p. 303, ago. 2001. Suplemento. Resumo. Apresentado no 34º Congresso Brasileiro de Fitopatologia, 2001.| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | VÉRAS, S. de M.; GASPAROTTO, L.; COSTA, S. B.; PEREIRA, J. C. R.; GATO, A.; BOHER, B. Comparação de métodos de inoculação de Ralstonia solanacearum Raça 2 em cultivares de banana. Fitopatologia Brasileira, v. 24, p. 259, ago. 1999. Suplemento. Edição dos resumos do 32º Congresso Brasileiro de Fitopatologia, 1999. Resumo 80.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
|  |
| 12. | | VÉRAS, S. de M.; GASPAROTTO, L.; COSTA, S. B.; PEREIRA, J. C. R.; GATO, A.; BOHER, B. Avaliação da resistência de diploides de banana à Ralstonia solanacearum Raça 2. Fitopatologia Brasileira, v. 24, p. 259, ago. 1999. Suplemento. Edição dos resumos do 32º Congresso Brasileiro de Fitopatologia, 1999. Resumo 79.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | SILVA, S. de O.; VERAS, S. de M.; GASPAROTTO, L.; MATOS, A. P. de; CORDEIRO, Z. M.; BOHER, B. Evaluation of Musa spp. for resistance to moko disease (Ralstonia solanacearum, race 2). Infomusa, v. 9, n. 1, p. 19-20, jun. 2000.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 15. | | SILVA, S. de O. e; VERAS, S. de M.; GASPAROTTO, L.; MATOS, A. P. de; CORDEIRO, Z. J. M.; BOHER, B. Evaluación de Musa spp. para la resistência a la enfermedad de Moko (Ralstonia solanacearum, raza 2). Infomusa, v. 9, n. 1, p. 19-20, jun. 2000.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 15 | |
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