| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
|
Data corrente: |
21/10/2014 |
|
Data da última atualização: |
02/04/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
AGUSTINI, B. C.; SILVA, L. P. da; BLOCH JUNIOR, C.; BONFIM, T. M. B.; SILVA, G. A. da. |
|
Afiliação: |
BRUNA CARLA AGUSTINI, CNPUV; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, CENARGEN; GILDO ALMEIDA DA SILVA, CNPUV. |
|
Título: |
Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 98, n. 12, p. 5645-5654, 2014. |
|
Idioma: |
Português |
|
Notas: |
DOI: 10.1007/s00253-014-5686-7 |
|
Conteúdo: |
Yeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database. This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts. MenosYeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After ma... Mostrar Tudo |
|
Palavras-Chave: |
Levedura; MALDI-TOF MS. |
|
Thesagro: |
Base de dados; Biologia molecular; Genética; Levedo; Microrganismo. |
|
Thesaurus Nal: |
Molecular biology; Yeasts. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110284/1/Silva-AMB.pdf
|
|
Marc: |
LEADER 02726naa a2200289 a 4500
001 1997892
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aAGUSTINI, B. C.
245 $aEvaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database.$h[electronic resource]
260 $c2014
500 $aDOI: 10.1007/s00253-014-5686-7
520 $aYeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database. This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts.
650 $aMolecular biology
650 $aYeasts
650 $aBase de dados
650 $aBiologia molecular
650 $aGenética
650 $aLevedo
650 $aMicrorganismo
653 $aLevedura
653 $aMALDI-TOF MS
700 1 $aSILVA, L. P. da
700 1 $aBLOCH JUNIOR, C.
700 1 $aBONFIM, T. M. B.
700 1 $aSILVA, G. A. da
773 $tApplied Microbiology and Biotechnology$gv. 98, n. 12, p. 5645-5654, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 221 | |
| 61. |  | SILVA, C. M.; STEPHAN, M. P.; AZEVEDO, T. de L.; BLOCH JUNIOR, C.; PRATES, M. V.; LAGO, R. C. A. HPLC fractionation of peptides obtained after partial hydrolysis of a whey protein isolate. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: abstracts. Foz do Iguaçu: IUFoST: ALACCTA, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 62. | | PIRES JUNIOR, O. R.; SEBBEN, A.; SCHWARTZ. E. F.; MORALES, R. A. V.; BLOCH JUNIOR, C.; SCHWARTZ, C. A. Further report of the occurrence of tetrodoxin and new analogues in the Anuran family Brachycephalidae. Toxicon, Elmsford, NY, v. 45, p. 73-79, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 63. | | RIBEIRO, A. C. J.; MAGALHÃES, M. T. Q.; SILVA, L. P.; PRATES, M. V.; BLOCH JUNIOR, C. Isolamento e caracterização de um peptídeo com potencial antimicrobiano da secreção cutânea de Hypsiboas punctatus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 048. p. 88.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 64. | | VINECKY, F.; BARBOSA, E. A.; BRITO, K. M.; PRATES, M. V.; BLOCH JÚNIOR, C.; ANDRADE, A. C. Isolamento de genes que codificam peptídeos antimicrobianos produzidos por hylidios da fauna brasileira. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 69.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 65. | | MELO, F. R.; FRANCO, O. L.; BLOCH JUNIOR, C.; SILVA, M. C. M.; SA, M. F. G. de. Isolation of cysteine proteinases from acanthoscelides obtectus say (coleoptera, bruchidae). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.34E-60.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 66. | | GERHARDT, I. R.; LEITE, A.; PAES, N.; ROMANO, E.; AZEVEDO, M.; BLOCH JUNIOR, C.; GROSSI de SA, M. F. Isolation and partial characterization of a genomic clone of arcelin-5 protein. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIA VEGETAL, 2., 1995, Puerto Iguazu, Argentina. REDBIO95. [S.l.: s.n.], 1995. n.D-11.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 67. | | FRANCO, O. L.; RIDGEN, D. J.; MELO, F. R.; BLOCH JUNIOR, C.; GROSSI-de- SÁ, M. F. Inibidores de alpha-amilase do trigo ativos contra alpha-amilases de bruquídeos e explicação estrutural das especificidades observadas. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 54.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 68. | | SILVA, D. P.; CASADO FILHO, E. L.; FARIAS, L. R.; BLOCH JÚNIOR, C.; NORONHA, E. F.; FRANCO, O. L. Inibidores ativos contra α-amilases de Callosobruchus maculatus provenientes de sementes de sucupira branca Pterodon pubescens. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO DE PLANTAS REGIONAL DF, 1., 2005, Brasília, DF. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 110. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 144).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 69. | | MADEIRA, L. M.; TINOCO, M. L. P.; VIANNA, G. R.; RECH, E. L.; BLOCH JUNIOR, C.; ARAGÃO, F. J. L. Expressão de genes de peptídeos antimicrobianos em soja [Glycine max L. (Merrill)] geneticamente modificada visando resistência à ferrugem asiática. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 623.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 71. | | WERMELINGER, L. S.; DUTRA, D. L.; OLIVEIRA-CARVALHO, A. L.; SOARES, M. R.; BLOCH JUNIOR, C.; ZINGALI, R. B. Fast analysis of low-molecular-weight compounds present in snake venom: identification of ten new pyroglutamate-containing peptides. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 73. | | SILVA, V. C.; MELO, J. A. T.; BARBOSA, E. A.; SILVA, L. P.; BLOCH JÚNIOR, C.; ANDRADE, A. C. Estudo integrado de genômica e proteômica associados à qualidade do café. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 78.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 74. | | GALDINO, A. S.; ULHOA, C. J.; MORAES, L. M. P.; PRATES, M. V.; BLOCH JÚNIOR, C.; TORRES, F. A. G. Cloning, molecular characterization and heterologous expression of AMY1, an α-amylase gene from Cryptococcus flavus. FEMS Microbiology Letters, Amsterdam, v. 280, p. 189-194, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 76. | | MANDEL, S. M. de S.; MUNDIM, N. C. C. R.; PRATES, M. V.; SILVA, L. P.; BLOCH JÚNIOR, C. Dermaseptins and phylloseptins de Phyllomedusa (Hylidae) secretion. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 77. | | FELICIANO SILVA, A. E. D.; DIAS, A. L.; UNANIAN, M. M.; FREITAS, A. R. de; BLOCH JUNIOR, C. Conteúdo de peptídeos e avaliação morfofisiológica dos espermatozóides de epidídimo e ejaculado de bovinos. Revista Brasileira de Zootecnia, v.32, n.6, (supl.2), p.1890-1900, 2003.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 80. | | COSTA, T. O. G.; MORALES, R. A. V.; BRITO, J. P.; GORDO, M.; PINTO, A. C.; BLOCH JUNIOR, C. Occurrence of bufotenin in the Osteocephalus genus (Anura: Hylidae). Toxicon, v. 46, n. 4, p. 371-374, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 221 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
