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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
15/03/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOARI, A. de J.; QUADROS, A. F. F.; KAUFFMANN, C. M.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; CORDOVIL, G. A.; GAVINHO, B. E. S.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, T. |
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Afiliação: |
ALESSANDRA DE JESUS BOARI, CPATU; AYANE F. F. QUADROS, UFRA; CATERYNNE M. KAUFFMANN, UFRA; KÉSSIA F. C. PANTOJA, Univesidade Estadual de São Paulo; RUI ALBERTO GOMES JUNIOR, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; RAIMUNDO PARENTE DE OLIVEIRA, CPATU; GABRIELE A. CORDOVIL, UFRA; BRENDA E. S. GAVINHO, UFRA; ELLIOT W. KITAJIMA, ESALQ/USP; ROSANA BLAWID, Universidade Federal Rural de Pernambuco; TATSUYA NAGATA, UNB. |
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Título: |
Near-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 46, n. 4, p. 487-491, Aug. 2021. |
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DOI: |
https://doi.org/10.1007/s40858-021-00420-w |
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Idioma: |
Inglês |
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Conteúdo: |
The sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%). MenosThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% ... Mostrar Tudo |
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Thesagro: |
Feijão; Genoma; Vigna Unguiculata; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02717naa a2200313 a 4500
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00420-w$2DOI
100 1 $aBOARI, A. de J.
245 $aNear-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection.$h[electronic resource]
260 $c2021
520 $aThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%).
650 $aFeijão
650 $aGenoma
650 $aVigna Unguiculata
650 $aVírus
700 1 $aQUADROS, A. F. F.
700 1 $aKAUFFMANN, C. M.
700 1 $aPANTOJA, K. F. C.
700 1 $aGOMES JUNIOR, R. A.
700 1 $aFREIRE FILHO, F. R.
700 1 $aOLIVEIRA, R. P. de
700 1 $aCORDOVIL, G. A.
700 1 $aGAVINHO, B. E. S.
700 1 $aKITAJIMA, E. W.
700 1 $aBLAWID, R.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 46, n. 4, p. 487-491, Aug. 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca |
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| Registros recuperados : 48 | |
| 4. |  | BLAWID, R.; MORGADO, F. S.; SOUZA, C. A.; BOITEUX, L. S.; PEREIRA-CARVALHO, R. C. Fluorescence in situ hybridization analysis of endosymbiont genera reveals novel infection patterns in a tomato-infesting Bemisia tabaci population from Brazil. Tropical Plant Pathology, Brasília, DF, v. 40, n. 4, p. 233-243, Aug. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | KAUFFMANN, C. M.; BOARI, A. de J.; SILVA, J. M. F.; BLAWID, R.; NAGATA, T. Complete genome sequence of mimosa mosaic virus, a new sobemovirus infecting Mimosa sensitiva L. Archives of Virology, v. 168, n. 1, Article number 28, Jan. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | KAUFFMANN, C. M.; BOARI, A. de J.; SILVA, J. M. F.; BLAWID, R.; NAGATA, T. Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil. Archives of Virology, v. 167, n. 12, p. 2817-2820, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| 9. | | SILVA, J. M. F.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. In silico detection of a defective genomic RNA of Grapevine leafroll-associated virus 4 strain 5 in High-Throughput Sequencing data. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 162-163.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | QUADROS, A. F. F.; KAUFFMANN, C. M.; BOARI, A. de J.; BLAWID, R.; NAGATA, T. Sequenciamento do genoma de um Cytorhabdovirus infectando patchouli. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019. p. 836.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | SILVA, J. M. F; RWAHNIH, M. A.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1. Virus Genes, v.53, n. 4, p. 667-671 [online], 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | KAUFFMANN, C. M.; BOARI, A. de J.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, R. Detecção de uma nova espécie de Fabavirus em pimenteira-do-reino por sequenciamento de alto desempenho. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019. p. 814.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | TAVARES, M. L.; BLAWID, R.; NAGATA, T.; NAGATA, A. K. I. Immunostaining of root tissue of nicotiana benthamiana infected with pepper risngpot virus. Virus Reviews and Research, v. 20, n. 2, p. 137, 2016. Edição dos resumos do XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016. p. 141. Na publicação: Inoue Nagata, A. K. Resumo PIV 223.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | SILVA, J. M. F.; FAJARDO, T. V. M.; AL RWAHNIH, M.; BLAWID, R.; NAGATA, T. Molecular characterization of grapevine enamo-like virus, a novel putative member of the genus enamovirus. Virus Reviews and Research, v. 20, n. 2, p. 27, 2016. (Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | SILVA, J. M. F.; FAJARDO, T. V. M.; AL RWAHNIN, M.; BLAWID, R.; NAGATA, T. Molecular characterization of Grapevine enamolike virus, a novel putative member of the genus Enamovirus. Virus Reviews and Research, n. 20, v. 2, p. 28-29, 2016. (Annals of the XXVII Brazilian Congress of Virology & X Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & X Mercosur Meeting of Virology, Pirenópolis, GO, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | TAVARES-ESASHIKA, M. L.; CAMPOS, R. N. S.; BLAWID, R.; LUZ, L. L. da; INOUE-NAGATA, A. K.; NAGATA, T. Characterization of an infectious clone of pepper ringspot virus and its use as a viral vector. Archives of Virology, v. 165, n. 2, p. 367-375, Feb. 2020.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 48 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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