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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/12/2015 |
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Data da última atualização: |
16/04/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. |
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Afiliação: |
ANTONIO NHANI JUNIOR, CNPT; LUCIANO CONSOLI, CNPT; ELISEU BINNECK, CNPSO. |
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Título: |
Suppression subtractive hybridization analysis of genes regulated by magnaporthe oryzae infection in wheat adult plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. |
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Páginas: |
p. 34. |
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Idioma: |
Inglês |
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Conteúdo: |
Blast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype. MenosBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For t... Mostrar Tudo |
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Thesagro: |
Trigo. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135952/1/suppression.binneck.2015.pdf
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Marc: |
LEADER 03294nam a2200193 a 4500
001 2032054
005 2018-04-16
008 2015 bl uuuu u00u1 u #d
100 1 $aTURCHETTO, C.
245 $aSuppression subtractive hybridization analysis of genes regulated by magnaporthe oryzae infection in wheat adult plants.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos].$c2015
300 $ap. 34.
520 $aBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype.
650 $aTrigo
700 1 $aGONZÁLES, H. H. S.
700 1 $aTORRES, G. A. M.
700 1 $aNHANI, A.
700 1 $aCONSOLI, L.
700 1 $aBINNECK, E.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 169 | |
| 23. |  | BUENO, P.; LAU, E. Y.; BINNECK, E.; NHANI JUNIOR, A.; SCAGLIUSI, S. M. M. RNA-Seq para análise de genes diferencialmente expressos em micrósporos de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2021. p. 48.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Soja. |
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| 24. | | BUENO, P.; LAU, E. Y.; BINNECK, E.; NHANI JUNIOR, A.; SCAGLIUSI, S. M. M. RNA-Seq para análise de genes diferencialmente expressos em micrósporos de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2021. p. 48.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 25. | | BINNECK, E.; SILVA, J. F. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. VSQual: a visual system to assist DNA sequencing quality control. Genetics and Molecular Research, Ribeirão Preto, v. 3, n. 4, p. 474-482, 2004.| Biblioteca(s): Embrapa Soja. |
|  |
| 26. | | TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. Suppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants. In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015]. p. 34.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 29. | | MITANI, E. A.; NASCIMENTO, L. S.; SILLA, P. R.; FARIAS, J. R. B.; BINNECK, E. Sistema Web para estimativas de perdas por seca na cultura da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 48-49. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 30. | | NASCIMENTO, L. S.; SILLA, P. R.; VITORINO, J. C.; IWATA, M.; MITANI, E. A.; BINNECK, E. Soybean gene express: plataforma para análise de expressão diferencial e bibliotecas substrativas de cDNA. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 105-109. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 31. | | COSTAMILAN, L.; BERTAGNOLLI, P.; YORINORI, J.; ALMEIDA, A.; SEIXAS, C.; BINNECK, E.; ARAÚJO, M.; CARBONARI, J. Soybean stem canker (Diaporthe phaseolorum var. caulivora) in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 250. Resumo P654. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 32. | | COSTAMILAN, L.; BERTAGNOLLI, P.; YORINORI, J.; ALMEIDA, A.; SEIXAS, C.; BINNECK, E.; ARAÚJO, M.; CARBONARI, J. Soybean stem canker (Diaporthe phasseolorum var. caulivora) in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 250, ref. P654. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 33. | | SILLA, P. R.; PASSIANOTTO, A. L. L.; CAMARGO-BRUNETTO, M. A. O.; GONELA, A.; MARCELINO, F. C.; BINNECK, E. Algoritmo computacional para determinar o perfil mínimo de marcadores moleculares que discriminam um conjunto de cultivares. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 133-135. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 34. | | SOSA-GÓMEZ, D. R.; OLIVEIRA, L. J. SILVA, J. J. da; MARIN, S. R. R.; BINNECK, E. Caracterização molecular de espécies de Scaptocoris (Hemiptera: Cydnidae). In: REUNIÃO SUL-BRASILEIRA SOBRE PRAGAS DE SOLO, 10., 2007, Dourados. Pragas-Solo-Sul: anais e ata. Dourados: Embrapa Agropecuária Oeste, 2007. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 88). Organizado por: Crébio José Ávila, Rômulo Penna Scorza Júnior.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 35. | | TURCHETTO, C.; GONZÁLES, H. H. S.; FERREIRA, J. R.; TORRES, G. A. M.; CONSOLI, L.; NHANI JUNIOR, A.; BINNECK, E. Prospecção de genes relacionados à interação de Magnaporthe oryzae com trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 10., MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 7., 2015, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2015. Pós-Graduação. p. 45.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 36. | | SOSA-GÓMEZ, D. R.; SILVA, J. J. da; COSTA, F.; BINNECK, E.; MARIN, S. R. R.; NEPOMUCENO, A. L. Population structure of the Brazilian southern green stink bug, Nezara viridula. Journal of Insect Science, 5, n. 23, p. 1-14, Oct. 2005.| Biblioteca(s): Embrapa Soja. |
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| 37. | | ZIOBER, I. L.; PAIÃO, F. G.; MARCHI, D. F.; COUTINHO, L. L.; BINNECK, E.; NEPOMUCENO, A. L.; SHIMOKOMAKI, M. Heat and chemical stress modulate the expression of the alfa-RYR gene in broiler chickens. Genetics and Molecular Research, Ribeirão Preto, v. 9, n. 2, p. 1258-1266, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 38. | | FUGANTI, R.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. Desenvolvimento de marcadores moleculares de microssatélite para seleção de genótipos de soja resistentes a Meloidogyne javanica. In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.025.| Biblioteca(s): Embrapa Soja. |
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| 39. | | SOUZA, T. D. de; GODOY, S. M. de; BINNECK, E.; FELICIANO, D. C.; SÓSA-GOMEZ, D. R. Desenvolvimento de microssatélites como ferramenta para estudo da diversidade genética do fungo entomopatogênico Metarhizium rileyi. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 28., 2022, Fortaleza. Biodiversidade: Conhecer, conservar e utilizar. anais. Fortaleza: Sociedade Entomológica do Brasil, 2022. Edição Técnica: Nivia da Silva Dias Pini, Gerane Celly Dias Bezerra Silva, Márcio Alves Silva, Regiane Cristina Oliveira, José Wagner da Silva Melo, Flávia Rabelo Barbosa. resumo. p. 324.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 40. | | NEPOMUCENO, A. L.; FARIAS, J. R. B.; NEUMAIER, N.; TOBITA, S.; YAMANAKA, N.; BINNECK, E.; MOLINA, J.; STOLF, R. Drought induced gene expression in Brazilian soybean genotypes. In: INTERNATIONAL CONFERENCE ON INTEGRATED APPROACHES TO SUSTAIN AND IMPROVE PLANT PRODUCTION UNDER DROUGHT STRESS, 2., 2005, Rome. InterDrought - II: final program and abstract book. Rome, 2005. Não paginado. Resumo: P 6.34.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 169 | |
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