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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
03/08/2016 |
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Data da última atualização: |
07/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIGLIOTI, A.; OLIVEIRA, H. N.; SANTANA, C. H.; IBELLI, A. M. G.; NÉO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. |
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Afiliação: |
Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); UFSCAR; Unesp (Jaboticabal); MARCIO DIAS RABELO, CPPSE; Unesp (Jaboticabal); LUCIANA GATTO BRITO, CPAF-Rondonia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 7, p. 657-66, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. MenosThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus a... Mostrar Tudo |
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Palavras-Chave: |
QPCR; R microplus; Resistance. |
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Thesaurus Nal: |
babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03159naa a2200277 a 4500
001 2050164
005 2022-03-07
008 2016 bl uuuu u00u1 u #d
100 1 $aGIGLIOTI, A.
245 $aBabesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil.$h[electronic resource]
260 $c2016
520 $aThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal.
650 $ababesiosis
653 $aQPCR
653 $aR microplus
653 $aResistance
700 1 $aOLIVEIRA, H. N.
700 1 $aSANTANA, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aNÉO, T. A.
700 1 $aBILHASSI, T. B.
700 1 $aRABELO, M. D.
700 1 $aMACHADO, R. Z.
700 1 $aBRITO, L. G.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 7, p. 657-66, 2016.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Unidades Centrais. |
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Data corrente: |
14/01/1997 |
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Data da última atualização: |
24/11/2011 |
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Autoria: |
DIAS-FILHO, M. B.; WISE, J. A.; DAWSON, T. E. |
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Afiliação: |
MOACYR BERNARDINO DIAS FILHO, CPATU; JAMES A. WISE, CORNELL UNIVERSITY; TODD E. DAWSON, CORNELL UNIVERSITY. |
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Título: |
Irradiance and water deficit effects on gas exchange behavior of two C3 Amazonian weeds. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 30, n. 3, p. 319-325, mar. 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
The gas exchange behavior or two perennial weed species, a vine Ipomoea asarifolia (Desr.) Roem. & Schultz and a shrub Stachytarpheta cayennensis (L.C. Rich) Vahl., both common in the Brazilian Amazonia, was compared for plants grown under well watered and water limited conditions. Under well-watered conditions, light saturated rates of photosynthesis (A) and stomatal conductance (g) in I. asarifolia were, respectively, 15 and 83% higher than in S. cayennensis. When water stress was imposed, A and g in I. asarifolia declined, respectively, by 29 and 57% in relation to S. cayennensis. Dark respiration and light compensation point were similar on well-watered plants. Under water limited conditions, S. cayennensis had 60% higher dark respiration and 35% higherlight compensation point than I. asarifolia. The observed responses are in accord with the known distribution of the species in the field and may provide important information for designing differential management strategies for controlling these weeds. |
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Palavras-Chave: |
Amazon; Ipomoea asarifolia; Tropical pasture. |
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Thesagro: |
Stachytarpheta Cayennensis. |
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Thesaurus NAL: |
photosynthesis; stomatal conductance; water stress. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/47798/1/pab95-04-mar.pdf
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Marc: |
LEADER 01710naa a2200229 a 4500
001 1907160
005 2011-11-24
008 1995 bl uuuu u00u1 u #d
100 1 $aDIAS-FILHO, M. B.
245 $aIrradiance and water deficit effects on gas exchange behavior of two C3 Amazonian weeds.
260 $c1995
520 $aThe gas exchange behavior or two perennial weed species, a vine Ipomoea asarifolia (Desr.) Roem. & Schultz and a shrub Stachytarpheta cayennensis (L.C. Rich) Vahl., both common in the Brazilian Amazonia, was compared for plants grown under well watered and water limited conditions. Under well-watered conditions, light saturated rates of photosynthesis (A) and stomatal conductance (g) in I. asarifolia were, respectively, 15 and 83% higher than in S. cayennensis. When water stress was imposed, A and g in I. asarifolia declined, respectively, by 29 and 57% in relation to S. cayennensis. Dark respiration and light compensation point were similar on well-watered plants. Under water limited conditions, S. cayennensis had 60% higher dark respiration and 35% higherlight compensation point than I. asarifolia. The observed responses are in accord with the known distribution of the species in the field and may provide important information for designing differential management strategies for controlling these weeds.
650 $aphotosynthesis
650 $astomatal conductance
650 $awater stress
650 $aStachytarpheta Cayennensis
653 $aAmazon
653 $aIpomoea asarifolia
653 $aTropical pasture
700 1 $aWISE, J. A.
700 1 $aDAWSON, T. E.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 30, n. 3, p. 319-325, mar. 1995.
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Embrapa Amazônia Oriental (CPATU) |
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