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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
03/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Kelma Costa de Souza, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE; ALICE ANDRIOLI, CNPC; LUCIA HELENA SIDER, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; Rosivaldo Quirino Bezerra Junior; Renato Mesquita Peixoto; Maria Fátima da Silva Teixeira. |
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Título: |
Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015. |
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Idioma: |
Português |
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Conteúdo: |
[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium. Menos[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene ... Mostrar Tudo |
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Palavras-Chave: |
Artrite-encefalite Caprina; CAEV; Caprine arthritis encephalit virus; DNA sequence; PCR. |
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Thesagro: |
Caprino; Doença animal; Saliva; Transmissão de doença; Vírus. |
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Thesaurus Nal: |
Animal diseases; Disease transmission; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136302/1/cnpc-2015-Deteccao.pdf
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Marc: |
LEADER 04687naa a2200349 a 4500
001 2032574
005 2019-04-03
008 2015 bl uuuu u00u1 u #d
100 1 $aSOUZA, K. C. de
245 $aDetecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva.$h[electronic resource]
260 $c2015
520 $a[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium.
650 $aAnimal diseases
650 $aDisease transmission
650 $aGoats
650 $aCaprino
650 $aDoença animal
650 $aSaliva
650 $aTransmissão de doença
650 $aVírus
653 $aArtrite-encefalite Caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aDNA sequence
653 $aPCR
700 1 $aANDRIOLI, A.
700 1 $aSIDER, L. H.
700 1 $aPINHEIRO, R. R.
700 1 $aBEZERRA JUNIOR, R. Q.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 43, n. 1266, p. 1-6, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 557 | |
| 141. |  | ALVES, T. M.; ROSA, A. da S.; QUINTELA, E. D.; BARRIGOSSI, J. A. F. Distribuição espacial do percevejo-do-colmo, Tibraca limbativentris Stal. 1860 (Heteroptera: pentatomidae), em arroz irrigado. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 6., 2009, Porto Alegre. Estresses e sustentabilidade: desafios para a lavoura arrozeira: anais. Porto Alegre: Palotti, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 143. | | LIMA, O. P.; ROSA, G. O.; POMPEO, M. L.; DIVIDORIO, C.; ROSA, A. do N. Efeito de diferentes doses de prostaglandina f2 alfa na inducao do estro e fertilidade de vacas e novilhas zebuinas. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 18., CONGRESSO INTERNACIONAL DE VETERINARIA EM LINGUA PORTUGUESA, 3.; SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 3., 1982, Balneario Camboriu. Resumos. Florianopolis : SBMV / SOMEVESC, 1982. p.236. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 144. | | EMYGDIO, B. M.; ROSA, A. P. S. A. da; BARROS, L.; FACCHINELLO, P. H. Efeito de diferentes épocas de semeadura sobre o desempenho de cultivares de sorgo sacarino em condições de solos hidromórficos. In: REUNIÃO TÉCNICA ANUAL DO MILHO, 59.; REUNIÃO TÉCNICA ANUAL DO SORGO, 42., 2014, Três de Maio. Atas e artigos. Três de Maio: EMATER/RS: FEPAGRO: SETREM, 2014. 182 p. Organização: Marcos Caraffa, Cinei Teresinha Riffel, Letícia dos Santos Holbig Harter. p. 116-117| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 145. | | MEDINA, L.; HELLIWIG, L.; LANDERO, E.; EMYGDIO, B. M.; ROSA, A. P. S. A. da. Determinação do número de instares de Spodoptera frugiperda (J.E. SMITH, 1797) (Lepidoptera: Noctuidae) em sorgo sacarino BRS 506 In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 151. | | BETTONI, T. V.; TORRES JÚNIOR, R. A. A.; SILVA, L. O. C.; ROSA, A. N. Uso de simulação na avaliação de estratégias de reposição de matrizes em programas de melhoramento de bovinos de corte. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 3., 2007, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2007. p. 44 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
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| 152. | | ROSA, A. M.; GALARZA, B. P.; MARODIN, G. A. B.; SANTOS, H. P. dos. Manejo de indutores de brotação em vinhedos de 'Merlot' (Vitis vinifera L.) na Região da Campanha Gaúcha. In: CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15.; CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 13., 2015, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho: Associação Brasileira de Enologia, 2015. p. 356 XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 157. | | ABREU, U. G. P. de; ROSA, A. N.; OLIVEIRA, C. A. L. Melhoramento genético da raça nelore no Pantanal. Corumbá, MS: Embrapa Pantanal, 2007. 4 p. (Embrapa Pantanal. Artigo de Divulgação na Mídia, 119). Disponível também em: clickpantanal, estadomsnews, bonitonoticias, portalms, folhadedourados, agrosoft, criareplantar, agrolink, perfilnews, douradosinforma, zoonews, infobibos, reporterms, corumbaonline, portalbonito, aquidauananews,...| Tipo: Artigo de Divulgação na Mídia | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 557 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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