Data/hora: 17/06/2024 08:04:48

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 04/10/2016
Data da última atualização: | 29/12/2017
Autoria: | SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R.
Afiliação: | PATRÍCIA C. SOUSA, UFERSA; ERIKA A. A. SANTOS, UFERSA; ANDRÉIA M. SILVA, UFERSA; JOSÉ A. B. BEZERRA, UFERSA; ANA L. P. SOUZA, UFERSA; GABRIELA L. LIMA, UFRR; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA.
Título: | Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016.
Idioma: | Inglês
Conteúdo: | The aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol.
Palavras-Chave: | Dano ultraestrutural e funcional; Euphractus sexcinctus; Membrane integrity; Mitochondrial activity; Six-banded armadillo; Sperm; Tatu-peba; Ultraestrutura; Ultrastructural and functional damages; Xenarthras.
Thesagro: | Criopreservação; Espermatozoide; Membrana; Mitocondria; Sêmen.
Thesaurus Nal: | Cryopreservation; Spermatozoa; Ultrastructure.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/148299/1/Identification-of-ultrastructural.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (60036-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS