Data/hora: 15/06/2024 18:25:31

Biblioteca(s): 	Embrapa Amazônia Oriental.	
Data corrente: 	02/01/2017	
Data da última atualização: 	20/05/2022	
Autoria: 	MOREIRA, C. M.; ANDRADE, H. B. de; BERTOLUCCI, S. K. V.; LAMEIRA, O. A.; MOHAMMED, A.; PINTO, J. E. B. P.	
Afiliação: 	Carolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA.	
Título: 	Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Turkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016.	
DOI: 	10.3906/biy-1601-56	
Idioma: 	Inglês	
Conteúdo: 	The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.	
Palavras-Chave: 	Regeneração in vitro.	
Thesagro: 	Curaua; Regeneração.	
Thesaurus Nal: 	thidiazuron.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/152619/1/Carolina-Moreira-Plantlet-regeneration-curaua-2016.pdf	
Registro original: 	Embrapa Amazônia Oriental (CPATU)	
Exemplares: 	CPATU - (53120-1) - UPC - AP - DD -  -  -  - DIS