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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia; Embrapa Roraima; Embrapa Semiárido; Embrapa Soja; Embrapa Solos; Embrapa Trigo; Embrapa Unidades Centrais. MenosEmbrapa Agrobiologia; Embrapa Agroindústria Tropical; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte... Mostrar Todas |
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Data corrente: |
22/10/2002 |
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Data da última atualização: |
19/09/2007 |
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Autoria: |
GUIMARÃES, P. M.; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J. |
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Título: |
Isolamento e caracterização de cDNAs codificadores para proteínas musculares do nematóide do cisto da soja, Heterodera glycines. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Brasília, DF: Embrapa Recursos Geneticos e Biotecnologia, 2001. |
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Páginas: |
22 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 20). |
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Idioma: |
Português |
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Conteúdo: |
Visando a identificação de genes ligados a interação nematóide-hospedeiro, realizou-se uma varredura de biblioteca de cDNA do nematóide Heterodera glycines em vetor À- UniZAPTM XR (Stratagene, La Jolla, CAL), utilizando anticorpos policlonais interespecíficos. Foi possível a clonagem e caracterização de dois cDNAs de Heterodera glycines que apresentaram alta identidade com genes que codificam para proteínas de filamento intermediário e para tropomiosinas de outros organismos, inclusive o nematóide de vida livre Caenorhabditis elegans e outros vermes. Estas seqüências foram denominadas Hg/F1 (proteína de filamento) e HgTrp 1 (tropomiosina) , respectivamente. |
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Palavras-Chave: |
cDNAs; Cesto da soja; Cisto da soja; Namatioide; Nematóide - cesto da soja; Nematóide - Cesto de soja; Nematóide do cisto da soja; Proteina muscular; Proteínas Moleculares; Proteinas musculares; Soybean. |
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Thesagro: |
Biologia; Biologia Molecular; Cisto; Engenharia Genética; Glycine Max; Heterodera Glycines; Hospedeiro; Meloidoginose; Nematóide; Proteína; Soja. |
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Thesaurus Nal: |
Meloidogyne; molecular biology; Nematoda; soybeans. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02084nam a2200469 a 4500
001 1185376
005 2007-09-19
008 2001 bl uuuu u0uu1 u #d
100 1 $aGUIMARÃES, P. M.
245 $aIsolamento e caracterização de cDNAs codificadores para proteínas musculares do nematóide do cisto da soja, Heterodera glycines.
260 $aBrasília, DF: Embrapa Recursos Geneticos e Biotecnologia$c2001
300 $a22 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 20).
520 $aVisando a identificação de genes ligados a interação nematóide-hospedeiro, realizou-se uma varredura de biblioteca de cDNA do nematóide Heterodera glycines em vetor À- UniZAPTM XR (Stratagene, La Jolla, CAL), utilizando anticorpos policlonais interespecíficos. Foi possível a clonagem e caracterização de dois cDNAs de Heterodera glycines que apresentaram alta identidade com genes que codificam para proteínas de filamento intermediário e para tropomiosinas de outros organismos, inclusive o nematóide de vida livre Caenorhabditis elegans e outros vermes. Estas seqüências foram denominadas Hg/F1 (proteína de filamento) e HgTrp 1 (tropomiosina) , respectivamente.
650 $aMeloidogyne
650 $amolecular biology
650 $aNematoda
650 $asoybeans
650 $aBiologia
650 $aBiologia Molecular
650 $aCisto
650 $aEngenharia Genética
650 $aGlycine Max
650 $aHeterodera Glycines
650 $aHospedeiro
650 $aMeloidoginose
650 $aNematóide
650 $aProteína
650 $aSoja
653 $acDNAs
653 $aCesto da soja
653 $aCisto da soja
653 $aNamatioide
653 $aNematóide - cesto da soja
653 $aNematóide - Cesto de soja
653 $aNematóide do cisto da soja
653 $aProteina muscular
653 $aProteínas Moleculares
653 $aProteinas musculares
653 $aSoybean
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aBERTIOLI, D. J.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/03/2016 |
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Data da última atualização: |
04/03/2016 |
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Autoria: |
LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. |
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Afiliação: |
VÂNIA F. LEMOS, UFRPE; EDUARDO L. S. GUARANÁ, UFRPE; JOSÉ A. B. AFONSO, UFRPE; JOSÉ J. FAGLIARI, UNESP; PAULO C. SILVA, UNESP; PIERRE C. SOARES, UFRPE; CARLA LOPES DE MENDONÇA, UFRPE. |
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Título: |
Effects of intramammary infection on whey proteinograms os sheep during lactation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. MenosThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for ... Mostrar Tudo |
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Palavras-Chave: |
Marcador biológico; Mastite subclínica; Proteína de fase aguda; Subclinical mastitis. |
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Thesagro: |
Leite; Ovelha. |
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Thesaurus NAL: |
Acute phase proteins; Biomarkers; Ewes; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140638/1/Effects-of-intramammary-infection.pdf
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Marc: |
LEADER 02780naa a2200313 a 4500
001 2039647
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLEMOS, V. F.
245 $aEffects of intramammary infection on whey proteinograms os sheep during lactation.
260 $c2015
520 $aThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.
650 $aAcute phase proteins
650 $aBiomarkers
650 $aEwes
650 $aMilk
650 $aLeite
650 $aOvelha
653 $aMarcador biológico
653 $aMastite subclínica
653 $aProteína de fase aguda
653 $aSubclinical mastitis
700 1 $aGUARANÁ, E. L. S.
700 1 $aAFONSO, J. A. B.
700 1 $aFAGLIARI, J. J.
700 1 $aSILVA, P. C.
700 1 $aSOARES P. C.
700 1 $aMENDONÇA, C. L. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 3, p. 230-236, mar. 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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