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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
22/12/1999 |
Data da última atualização: |
22/12/1999 |
Autoria: |
BERNARDES FILHO, R.; FORATO, L. A.; COLNAGO, L. A. |
Título: |
Evaluation of the quantitatives methods to determine the secondary structure of proteins by FTIR. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu, MG. Programa e resumos... [S.l.]: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1996. |
Páginas: |
p.129. Ref.M-56. |
Idioma: |
Inglês |
Conteúdo: |
The quantification of secondary structure from infrared bands is not simple and present serious problems. In this paper, we evaluate the quantitatives aspects of "resolution enhancement" and "pattern recognition" methods. In the first case we studied the Fourier deconvolution technique to enhance the resolution and use a fitting program to resolve the individuals bands. The results show that this method is not adequate to quantify the secondary structures. The major problems are the strong influence of the operator in selecting the parameters to fit the data, the correct assignment of the bands, etc. We have observed up to 50% in error using this method. The pattern recognition technique is based on a partial least squares method. It is necessary to obtain a calibration matrix using the infrared spectra and the proportions of secondary structure from X rays. The user almost does not interfere in the process.The problems are the definition of the types of secondary structures to use (alfa helix, beta sheet, beta turns, etc) and chose the method for calculate them from X rays data bank. We tested Levitt & Greer; Kabsch & Sander, Kabsch & Sander Modified algorithms to calculate secondary structures and the FTIR results show a better correlation when we use alfa helix, beta sheet and undefined structures with Levitt & Greer calculation. |
Palavras-Chave: |
Estrutura secundaria; FTIR; Protein; Secondary structure. |
Thesagro: |
Proteína. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02118naa a2200217 a 4500 001 1026966 005 1999-12-22 008 1996 bl --- 0-- u #d 100 1 $aBERNARDES FILHO, R. 245 $aEvaluation of the quantitatives methods to determine the secondary structure of proteins by FTIR. 260 $c1996 300 $ap.129. Ref.M-56. 520 $aThe quantification of secondary structure from infrared bands is not simple and present serious problems. In this paper, we evaluate the quantitatives aspects of "resolution enhancement" and "pattern recognition" methods. In the first case we studied the Fourier deconvolution technique to enhance the resolution and use a fitting program to resolve the individuals bands. The results show that this method is not adequate to quantify the secondary structures. The major problems are the strong influence of the operator in selecting the parameters to fit the data, the correct assignment of the bands, etc. We have observed up to 50% in error using this method. The pattern recognition technique is based on a partial least squares method. It is necessary to obtain a calibration matrix using the infrared spectra and the proportions of secondary structure from X rays. The user almost does not interfere in the process.The problems are the definition of the types of secondary structures to use (alfa helix, beta sheet, beta turns, etc) and chose the method for calculate them from X rays data bank. We tested Levitt & Greer; Kabsch & Sander, Kabsch & Sander Modified algorithms to calculate secondary structures and the FTIR results show a better correlation when we use alfa helix, beta sheet and undefined structures with Levitt & Greer calculation. 650 $aProteína 653 $aEstrutura secundaria 653 $aFTIR 653 $aProtein 653 $aSecondary structure 700 1 $aFORATO, L. A. 700 1 $aCOLNAGO, L. A. 773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu, MG. Programa e resumos... [S.l.]: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1996.
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Embrapa Instrumentação (CNPDIA) |
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27. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRITTO, D. de; BERNARDES FILHO, R.; ASSIS, O. B. G. Caracterização morfológica de filmes de derivados de quitosana por AFM e MEV. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 165-167. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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40. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERNANDES, C. P.; CORRÊA, T. R. A.; BERNARDES FILHO, R. Propriedades mêcanicas de filmes à base de zeína e nanofibras de celulose para revestimento de pêras e maçãs. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 99-101. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
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