| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
22/02/2006 |
|
Data da última atualização: |
23/02/2006 |
|
Autoria: |
BENEDITO, V. A.; KRONENBURG-van der Ven, B. C. E. van; TUYL, J. M. van; ANGENENT, G. C.; KRENS, F. A. |
|
Título: |
Transformation of Lilium longiflorum via particle bombardiment and generation of herbicide-resistant plants. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 3, p. 259-264, Sept. 2005. |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study is a contribution to the improvement of lily (Lilium longiflorum Thunb.) transformation procedures. A vector carrying the Arabidopsis SUPERMAN gene driven by the petunia flower-specific FLORAL BINDING PROTEIN 1 promoter and the resistance gene phosphinothricin acetyltransferase against herbicide bialaphos under the CaMV35S promoter was used to transform bulblet slices by particle bombardment. Our aims were to improve the transformation parameters for lily transformation in order to enhance the efficiency and to create novel phenotypes in lily flowers using transcription factors originating from dicot plants. We managed to obtain transgenic lines expressing in vitro resistance to bialaphos. The transgenic plants were transferred to the greenhouse, grown and monitored for two flowering seasons. Flowers derived from these plants appeared normal and indistinguishable from wild-type flowers.. |
|
Palavras-Chave: |
ABCDE model; Desenvolvimento floral; Fator de transcrição; Floral development; Gene homeótico; Homeotic gene; Lily; Modelo ABCDE; Transcription factor. |
|
Thesagro: |
Lírio. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01793naa a2200289 a 4500
001 1203799
005 2006-02-23
008 2005 bl uuuu u00u1 u #d
100 1 $aBENEDITO, V. A.
245 $aTransformation of Lilium longiflorum via particle bombardiment and generation of herbicide-resistant plants.
260 $c2005
520 $aThis study is a contribution to the improvement of lily (Lilium longiflorum Thunb.) transformation procedures. A vector carrying the Arabidopsis SUPERMAN gene driven by the petunia flower-specific FLORAL BINDING PROTEIN 1 promoter and the resistance gene phosphinothricin acetyltransferase against herbicide bialaphos under the CaMV35S promoter was used to transform bulblet slices by particle bombardment. Our aims were to improve the transformation parameters for lily transformation in order to enhance the efficiency and to create novel phenotypes in lily flowers using transcription factors originating from dicot plants. We managed to obtain transgenic lines expressing in vitro resistance to bialaphos. The transgenic plants were transferred to the greenhouse, grown and monitored for two flowering seasons. Flowers derived from these plants appeared normal and indistinguishable from wild-type flowers..
650 $aLírio
653 $aABCDE model
653 $aDesenvolvimento floral
653 $aFator de transcrição
653 $aFloral development
653 $aGene homeótico
653 $aHomeotic gene
653 $aLily
653 $aModelo ABCDE
653 $aTranscription factor
700 1 $aKRONENBURG-van der Ven, B. C. E. van
700 1 $aTUYL, J. M. van
700 1 $aANGENENT, G. C.
700 1 $aKRENS, F. A.
773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 5, n. 3, p. 259-264, Sept. 2005.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
