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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/03/2012 |
Data da última atualização: |
19/01/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. |
Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
Título: |
Ubiquitous urease affects soybean susceptibility to fungi. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012. |
DOI: |
DOI 10.1007/s11103-012-9894-1 |
Idioma: |
Inglês |
Conteúdo: |
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. MenosThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of u... Mostrar Tudo |
Palavras-Chave: |
Penicillium herguei; Transformação genética. |
Thesagro: |
Fungo; Phomopsis; Queima da saia; Soja. |
Thesaurus Nal: |
Genetic transformation; Soybeans. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02843naa a2200421 a 4500 001 1917463 005 2017-01-19 008 2012 bl uuuu u00u1 u #d 024 7 $aDOI 10.1007/s11103-012-9894-1$2DOI 100 1 $aWIEBKE-STROHM, B. 245 $aUbiquitous urease affects soybean susceptibility to fungi. 260 $c2012 520 $aThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. 650 $aGenetic transformation 650 $aSoybeans 650 $aFungo 650 $aPhomopsis 650 $aQueima da saia 650 $aSoja 653 $aPenicillium herguei 653 $aTransformação genética 700 1 $aPASQUALI, G. 700 1 $aMARGIS-PINHEIRO, M. 700 1 $aBENCKE, M. 700 1 $aBÜCKER-NETO, L. 700 1 $aBECKER-RITT, A. B. 700 1 $aMARTINELLI, A. H. S. 700 1 $aRECHENMACHER, C. 700 1 $aPOLACCO, J. C. 700 1 $aSTOLF, R. 700 1 $aMARCELINO, F. C. 700 1 $aABDELNOOR, R. V. 700 1 $aHOMRICH, M. S. 700 1 $aDEL PONTE, E. M. 700 1 $aCARLINI, C. R. 700 1 $aCARVALHO, M. C. C. G. de 700 1 $aBODANESE-ZANETTINI, M. H. 773 $tPlant Molecular Biology, The Hague$gv. 79, n. 1/2, p. 75-87, May 2012.
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Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Soja; Embrapa Unidades Centrais. |
Data corrente: |
21/06/2018 |
Data da última atualização: |
25/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MORAES, M. T. de; LEVIEN, R.; TREIN, C. R.; BONETTI, J. de A.; DEBIASI, H. |
Afiliação: |
CENA/USP; UFRGS; UFRGS; IFPR; HENRIQUE DEBIASI, CNPSO. |
Título: |
Corn crop performance in an Ultisol compacted by tractor traffic. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa agropecuária brasileira, Brasília, v. 53, n. 4, p. 464-477, Apr. 2018. |
DOI: |
10.1590/S0100-204X2018000400008 |
Idioma: |
Inglês |
Notas: |
Título em português: Desempenho da cultura do milho em Argissolo Vermelho compactado pelo tráfego agrícola. |
Conteúdo: |
The objective of this work was to determine whether compaction by tractor traffic in areas managed under controlled traffic can be limiting to corn crop, under different tillage systems, in a Typic Paleudult of medium texture. Two experiments were carried out, one in the field over two crop seasons and another in a greenhouse. The treatments consisted of minimum tillage with chiselling; no-tillage subjected to one, three, or six passes of a tractor weighing 3.8 Mg; and an area without traffic. Evaluations were performed for soil physicohydraulic parameters (soil bulk density, penetration resistance, and water retention curve), root and shoot growth, and grain yield. The agricultural traffic increased bulk density, soil penetration resistance, and water content at field capacity. The highest values for soil penetration resistance (1,600 kPa) and bulk density (1.67 g cm-3) in the trafficked soil were not limiting to corn development and increased grain yield for both crop seasons. Tractor traffic of up to six passes is beneficial to corn cultivation, and it increases water availability and corn grain yield. |
Palavras-Chave: |
Crescimento radicular; Produtividade de grão; Sistema plantio direto. |
Thesagro: |
Compactação do Solo; Milho; Plantio Direto; Produtividade; Sistema Radicular; Zea Mays. |
Thesaurus NAL: |
Compacted soils; Corn; Direct seeding; Grain yield; Grains; No-tillage; Root growth; Soil compaction. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191337/1/Moraes-Corn-Crop-open.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178859/1/Corn-crop-performance-in-an-ultisol.pdf
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Marc: |
LEADER 02335naa a2200397 a 4500 001 2104792 005 2019-01-25 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1590/S0100-204X2018000400008$2DOI 100 1 $aMORAES, M. T. de 245 $aCorn crop performance in an Ultisol compacted by tractor traffic.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Desempenho da cultura do milho em Argissolo Vermelho compactado pelo tráfego agrícola. 520 $aThe objective of this work was to determine whether compaction by tractor traffic in areas managed under controlled traffic can be limiting to corn crop, under different tillage systems, in a Typic Paleudult of medium texture. Two experiments were carried out, one in the field over two crop seasons and another in a greenhouse. The treatments consisted of minimum tillage with chiselling; no-tillage subjected to one, three, or six passes of a tractor weighing 3.8 Mg; and an area without traffic. Evaluations were performed for soil physicohydraulic parameters (soil bulk density, penetration resistance, and water retention curve), root and shoot growth, and grain yield. The agricultural traffic increased bulk density, soil penetration resistance, and water content at field capacity. The highest values for soil penetration resistance (1,600 kPa) and bulk density (1.67 g cm-3) in the trafficked soil were not limiting to corn development and increased grain yield for both crop seasons. Tractor traffic of up to six passes is beneficial to corn cultivation, and it increases water availability and corn grain yield. 650 $aCompacted soils 650 $aCorn 650 $aDirect seeding 650 $aGrain yield 650 $aGrains 650 $aNo-tillage 650 $aRoot growth 650 $aSoil compaction 650 $aCompactação do Solo 650 $aMilho 650 $aPlantio Direto 650 $aProdutividade 650 $aSistema Radicular 650 $aZea Mays 653 $aCrescimento radicular 653 $aProdutividade de grão 653 $aSistema plantio direto 700 1 $aLEVIEN, R. 700 1 $aTREIN, C. R. 700 1 $aBONETTI, J. de A. 700 1 $aDEBIASI, H. 773 $tPesquisa agropecuária brasileira, Brasília$gv. 53, n. 4, p. 464-477, Apr. 2018.
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