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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
Data corrente: |
22/08/2022 |
Data da última atualização: |
22/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NICIURA, S. C. M.; BENAVIDES, M. V.; OKINO, C. H.; IBELLI, A. M. G.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; ALESSANDRO PELEGRINE MINHO, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Pathogens, v. 11, n. 8, aug. 2022, 939. |
Páginas: |
12 p. |
DOI: |
https://doi.org/10.3390/pathogens11080939 |
Idioma: |
Inglês |
Conteúdo: |
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. MenosAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for... Mostrar Tudo |
Palavras-Chave: |
Gastrointestinal nematode control; GWAS; Molecular markers; Ovine; Parasite resistance. |
Thesagro: |
Marcador Molecular; Ovino; Parasito. |
Thesaurus Nal: |
Genotyping. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145606/1/GenomeWideAssociation.pdf
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Marc: |
LEADER 02449naa a2200325 a 4500 001 2145606 005 2022-08-22 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/pathogens11080939$2DOI 100 1 $aNICIURA, S. C. M. 245 $aGenome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.$h[electronic resource] 260 $c2022 300 $a12 p. 520 $aAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. 650 $aGenotyping 650 $aMarcador Molecular 650 $aOvino 650 $aParasito 653 $aGastrointestinal nematode control 653 $aGWAS 653 $aMolecular markers 653 $aOvine 653 $aParasite resistance 700 1 $aBENAVIDES, M. V. 700 1 $aOKINO, C. H. 700 1 $aIBELLI, A. M. G. 700 1 $aMINHO, A. P. 700 1 $aESTEVES, S. N. 700 1 $aCHAGAS, A. C. de S. 773 $tPathogens$gv. 11, n. 8, aug. 2022, 939.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
17/03/2010 |
Data da última atualização: |
08/06/2011 |
Tipo da produção científica: |
Documentos |
Autoria: |
BELTRAO, N. E. de M.; SILVA, C. A. D. da; SANTOS, J. W. dos; LIMA. M. M. de A.; SILVA, I. C. da; ROCHA, R. W. da C. |
Afiliação: |
NAPOLEAO ESBERARD DE MACEDO BELTRAO, CNPA; Carlos Alberto Domingues da Silva, Embrapa Algodão; José Wellington dos Santos, Embrapa Algodão; Marleide Magalhães de Andrade Lima, Embrapa Algodão; Ivanilda Cardoso da Silva, Embrapa Algodão; RENATO WAGNER DA COSTA ROCHA, CNPA. |
Título: |
ENCONTRO DE PRODUÇÃO CIENTÍFICA DA EMBRAPA ALGODÃO - EPC 2009, 4., 2009, Campina Grande. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2009. |
Páginas: |
55 p. |
Série: |
(Embrapa Algodão. Documentos, 227). |
ISSN: |
0103-0205 |
Idioma: |
Português |
Palavras-Chave: |
Encontro de Produção Científica; EPC; Metodologia Científica; Pesquisa Científica. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/17681/1/DOC227.PDF
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Marc: |
LEADER 00742nam a2200241 a 4500 001 1661590 005 2011-06-08 008 2009 bl uuuu 00u1 u #d 022 $a0103-0205 100 1 $aBELTRAO, N. E. de M. 245 $aENCONTRO DE PRODUÇÃO CIENTÍFICA DA EMBRAPA ALGODÃO - EPC 2009, 4., 2009, Campina Grande. 260 $aCampina Grande: Embrapa Algodão$c2009 300 $a55 p. 490 $a(Embrapa Algodão. Documentos, 227). 653 $aEncontro de Produção Científica 653 $aEPC 653 $aMetodologia Científica 653 $aPesquisa Científica 700 1 $aSILVA, C. A. D. da 700 1 $aSANTOS, J. W. dos 700 1 $aLIMA. M. M. de A. 700 1 $aSILVA, I. C. da 700 1 $aROCHA, R. W. da C.
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Embrapa Algodão (CNPA) |
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