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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/08/2023 |
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Data da última atualização: |
29/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHOCOBAR, M. L. E.; BELLO, H. J. S.; BASSETTO, C. C.; SILVA-MARINHO, N. M.; SATO, L. M. N.; SPERB, C.; CHAGAS, A. C. de S.; AMARANTE, A. F. T. |
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Afiliação: |
M. L. E. CHOCOBAR, São Paulo State University; H. J. S. BELLO, São Paulo State University; C. C. BASSETTO, São Paulo State University; N. M. SILVA-MARINHO, São Paulo State University; L. M. N. SATO, São Paulo State University; C. SPERB, São Paulo State University; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; A. F. T. AMARANTE, São Paulo State University. |
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Título: |
Biotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 227, oct. 2023, 107063. |
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Páginas: |
10 p. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2023.107063 |
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Idioma: |
Inglês |
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Conteúdo: |
The high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), levamisole (LEV), monepantel (MON) and ivermectin (IVE) was evaluated using the Larval Development Assay (RESISTA-Test©) 160 days PI. The resistant isolate showed a high resistance factor (RF) to TIA (141), LEV (822), MON (34) and IVE (196), while the RF of the susceptible isolate remained below three, which indicates high susceptibility to all drugs tested. Our study demonstrates that the susceptible isolate was able to infect lambs and did not show higher pathogenicity nor any differences on its biotic potential when compared to the resistant field isolate. These results indicate that the susceptible isolate has the potential to be used to replace a resistant population of H. contortus, which should be validated in long-term studies under commercial sheep farming conditions. MenosThe high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), le... Mostrar Tudo |
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Palavras-Chave: |
Anthelmintic Resistance; Nematodes; Parasitic Control; Refugia. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03176naa a2200277 a 4500
001 2156181
005 2023-08-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2023.107063$2DOI
100 1 $aCHOCOBAR, M. L. E.
245 $aBiotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil.$h[electronic resource]
260 $c2023
300 $a10 p.
520 $aThe high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), levamisole (LEV), monepantel (MON) and ivermectin (IVE) was evaluated using the Larval Development Assay (RESISTA-Test©) 160 days PI. The resistant isolate showed a high resistance factor (RF) to TIA (141), LEV (822), MON (34) and IVE (196), while the RF of the susceptible isolate remained below three, which indicates high susceptibility to all drugs tested. Our study demonstrates that the susceptible isolate was able to infect lambs and did not show higher pathogenicity nor any differences on its biotic potential when compared to the resistant field isolate. These results indicate that the susceptible isolate has the potential to be used to replace a resistant population of H. contortus, which should be validated in long-term studies under commercial sheep farming conditions.
653 $aAnthelmintic Resistance
653 $aNematodes
653 $aParasitic Control
653 $aRefugia
700 1 $aBELLO, H. J. S.
700 1 $aBASSETTO, C. C.
700 1 $aSILVA-MARINHO, N. M.
700 1 $aSATO, L. M. N.
700 1 $aSPERB, C.
700 1 $aCHAGAS, A. C. de S.
700 1 $aAMARANTE, A. F. T.
773 $tSmall Ruminant Research$gv. 227, oct. 2023, 107063.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 13 | |
| 1. |  | BASSETTO, C. C.; LINS, J. G. G.; SILVA, N. M. M.; BELLO, H. J. S.; CHOCOBAR, M. L. E.; AMARANTE, A. F. T.; CHAGAS, A. C. de S. Detecção da resistência anti-helmíntica em ovinos do estado de São Paulo. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 21., Ilhéus, 2022. Ilhéus: CBPV, 2023. p. 267.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 2. | | BELLO, H. J. S.; SANTOS, J. DE C.; KAPRITCHKOFF, R. T. I.; FIORENTINO, M. I. M.; MELITO, G. R.; DONATONI, F. A. B.; NICIURA, S. C. M.; ESTEVES, S. N.; MATOS, R. S.; CHAGAS, A. C. de S. Avaliação do OPG e frequência de tratamentos com anti-helmínticos em cordeiros submetidos à substituição parasitária de Haemonchus contortus. In: CONGRESSO PAULISTA DE PARASITOLOGIA, 10., Jaboticabal, 2023. Jaboticabal: Sociedade Paulista de Parasitologia, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | BASSETTO, C. C.; LINS, J. G. G.; ALBUQUERQUE, A. C. A.; SILVA, N. M. M. DA; BELLO, H. J. S.; CHOCOBAR, M. L. E.; AMARANTE, A. F. T. DO; CHAGAS, A. C. de S. Atualização da resistência anti-helmíntica em ovinos do estado de São Paulo. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 14., 2022, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2022. p. 19. (Embrapa Instrumentação. Documentos, 73).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | BASSETTO, C. C.; CHOCOBAR, M. L. E.; GAINZA, Y. A.; SANTOS, L. A. L.; BELLO, H. J. S.; SILVA, N. M. M.; AMARANTE, A. F. T.; CHAGAS, A. C. de S. Anthelmintic resistance diagnosis of Haemonchus contortus isolates through Resista-Test©. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 21., Ilhéus, 2022. Ilhéus: CBPV, 2023. p. 344.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | KAPRITCHKOFF, R. T. I.; OKINO, C. H.; NICIURA, S. C. M.; BELLO, H. J. S.; MATOS, R. S.; MELITO, G. R.; DONATONI, F. A. B.; ESTEVES, S. N.; CHAGAS, A. C. de S. Association of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds. Veterinary Parasitology, v. 328, June 2024, 110163. 8 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | CHOCOBAR, M. L. E.; BELLO, H. J. S.; BASSETTO, C. C.; SILVA-MARINHO, N. M.; SATO, L. M. N.; SPERB, C.; CHAGAS, A. C. de S.; AMARANTE, A. F. T. Biotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil. Small Ruminant Research, v. 227, oct. 2023, 107063. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | MELITO, G. R.; BELLO, H. J. S.; KAPRITCHKOFF, R. T. I.; FIORENTINO, M. I. M.; NICIURA, S. C. M.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S.; OKINO, C. H. Quantificação da expressão de genes de resposta imune local em ovinos de diferentes haplótipos da beta-globina. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 15., 2023, São Carlos, SP. Anais [...]. São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2023. p. 24. (Embrapa Pecuária Sudeste. Eventos Técnicos & Científicos, 1)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | BELLO, H. J. S.; SANTOS, J. DE C.; KAPRITCHKOFF, R. T. I.; FIORENTINO, M. I. M.; MELITO, G. R.; DONATONI, F. A. B.; NICIURA, S. C. M.; ESTEVES, S. N.; MATOS, R. S.; CHAGAS, A. C. de S. Influência da substituição parasitária de Haemonchus contortus no ganho de peso de cordeiros. In: CONGRESSO PAULISTA DE PARASITOLOGIA, 10., Jaboticabal, 2023. Jaboticabal: Sociedade Paulista de Parasitologia, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | KAPRITCHKOFF, R. T. I.; OKINO, C. H.; MATOS, R. S.; BELLO, H. J. S.; MELITO, G. R.; DONATONI, F. A. B.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Efeito do polimorfismo da β-globina na anemia por Hemoncose em ovinos de diferentes raças. In: CONGRESSO PAULISTA DE PARASITOLOGIA, 10., Jaboticabal, 2023. Jaboticabal: Sociedade Paulista de Parasitologia, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | BELLO, H. J. S.; KAPRITCHKOFF, R. T. I.; MELITO, G. R.; FIORENTINO, M. I. M.; SANTOS, J. de C.; DONATONI, F. A. B.; MATOS, R. S.; ESTEVES, S. N.; CHAGAS, A. C. de S. Eficácia do tratamento anti-helmíntico em ovinos após substituição parasitária com isolado suscetível Haemonchus contortus. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 15., 2023, São Carlos, SP. Anais [...]. São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2023. p. 27. (Embrapa Pecuária Sudeste. Eventos Técnicos & Científicos, 1)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | KAPRITCHKOFF, R. T. I.; OKINO, C. H.; BELLO, H. J. S.; MELITO, G. R.; FIORENTINO, M. I. M.; SANTOS, J. DE C.; DONATONI, F. A. B.; BARIONI JUNIOR, W.; ESTEVES, S. N.; CHAGAS, A. C. de S. Ovine B-globin polymorphisms and resistance to haemonchosis in different breeds. In: BRAZILIAN CONGRESS OF GENETICS, 68., 2023, Ouro Preto. Abstracts. Ribeirão Preto: SBG, 2023. p. 364.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | OKINO, C. H.; BELLO, H. J. S.; KAPRITCHOKOFF, R. T. I.; MELITO, G. R.; FIORENTINO, M. I. M.; DONATONI, F. A. B.; NICIURA, S. C. M.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Ovine resistance against Haemonchus contortus-globin subtype feature? In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 13., 2023, Satellite Meeting. Programme & Abstracts... Kruger Park/South Africa: IVIS, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | BASSETTO, C. C.; ALBUQUERQUE, A. C. A.; LINS, J. G. G.; SILVA, N. M. M.; CHOCOBAR, M. L. E.; BELLO, H. J. S.; MENA, M. O.; NICIURA, S. C. M.; AMARANTE, A. F. T.; CHAGAS, A. C. de S. Revisiting anthelmintic resistance in sheep flocks from São Paulo State, Brazil. International Journal for Parasitology: Drugs and Drug Resistance, v. 24, apr. 2024, 100527. 8 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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