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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/04/2004 |
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Data da última atualização: |
20/04/2004 |
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Autoria: |
LI, H; HAN, Y; BEECHING, J. R. |
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Título: |
Phenylalanine ammonia-lyase gene organisation, structure and activity in cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Salvador, v.17, p.87, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Post-harvest physiological deterioration (PPD) is a major constraint on the marketability and use of cassava. The physiological and biochemical changes observed during PPD show strong parallels with wound responses in other plants, except for the lack of extensive wound healing. Increased gene and enzyme activity of phenylalanine ammonia-lyase (PAL) has been observed in cassava roots during PPD. PAL is the first and key enzyme of the core pathway of phenylpronoid metabolism which plays pleiotropic roles in development and stress responses, including the production of isoflavonoid phytoalexins, flavonoid pigments, UV protectants, lignin, suberin and signalling molecules such as salicylic acid . We have isolated and sequenced two PAL CDNA clones (CPAL l and cPAL2) and a genomic clone (gPAL2). The genomic clone includes a 900 bp promoter region and a 71 1bp intron. The fine structure of the gene and the activity of the promoter are discussed. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01392naa a2200145 a 4500
001 1651974
005 2004-04-20
008 1998 bl uuuu u00u1 u #d
100 1 $aLI, H
245 $aPhenylalanine ammonia-lyase gene organisation, structure and activity in cassava.
260 $c1998
520 $aAbstract: Post-harvest physiological deterioration (PPD) is a major constraint on the marketability and use of cassava. The physiological and biochemical changes observed during PPD show strong parallels with wound responses in other plants, except for the lack of extensive wound healing. Increased gene and enzyme activity of phenylalanine ammonia-lyase (PAL) has been observed in cassava roots during PPD. PAL is the first and key enzyme of the core pathway of phenylpronoid metabolism which plays pleiotropic roles in development and stress responses, including the production of isoflavonoid phytoalexins, flavonoid pigments, UV protectants, lignin, suberin and signalling molecules such as salicylic acid . We have isolated and sequenced two PAL CDNA clones (CPAL l and cPAL2) and a genomic clone (gPAL2). The genomic clone includes a 900 bp promoter region and a 71 1bp intron. The fine structure of the gene and the activity of the promoter are discussed.
700 1 $aHAN, Y
700 1 $aBEECHING, J. R.
773 $tRevista Brasileira de Mandioca, Salvador$gv.17, p.87, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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