Data/hora: 17/06/2024 02:11:57

Biblioteca(s): 	Embrapa Florestas.	
Data corrente: 	02/07/1999	
Data da última atualização: 	20/05/2024	
Autoria: 	FINER, J. J.; KRIEBEL, H. B.; BECWAR, M. R.	
Título: 	Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.).	
Ano de publicação: 	1989	
Fonte/Imprenta: 	Plant Cell Reports, Berlin, v. 8, n. 4, p. 203-206, 1989.	
Idioma: 	Inglês	
Conteúdo: 	Immature female gametophytes were placed on a medium containing 50 mg/litre glutamine, 500 mg/litre casein hydrolysate, 3% sucrose, 2 mg/litre 2,4-D, 1 mg/litre BA [benzyladenine] and 0.2% Gelrite as a solidifying agent. Cultures were initiated and maintained at 230C in the dark. Embryogenic calli could be seen as early as 5 days following culture. Histological studies indicated proliferation of pre-existing embryogenic tissue in the corrosion cavity followed by extrusion of embryogenic callus through the micropylar end of the gametophyte. Embryogenic suspension cultures were obtained by placing embryogenic callus into liquid medium. Suspension cultures were subcultured weekly and proliferated as early-stage embryos with attached suspensors. Embryo development was obtained following transfer of the embryogenic tissue to an auxin-free medium containing 50 mM glutamine, 38 5M ABA and 6% sucrose. Although embryo development could be consistently obtained, whole plants have not yet been recovered from these somatic embryos.	
Palavras-Chave: 	2; 4 D; Cultura; Embriogenese somatica; Growth inhibitors; Pines; Plant growth regulators; Techniques.	
Thesagro: 	Célula; Pinus Strobus; Tecido.	
Thesaurus Nal: 	abscisic acid; benzyladenine; biotechnology; callus; conifers; cytokinins; growth regulators; tissue culture.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Florestas (CNPF)	
Exemplares: 	CNPF - (1039-1) - ADC - AP - DD -  -  -  - DIS