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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
09/03/1998 |
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Data da última atualização: |
09/03/1998 |
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Autoria: |
KONAN, N. K.; SCHOPKE, C.; CARCAMO, R.; BEACHY, R. N.; FAUQUET, C. |
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Título: |
An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Cell Reports, v.16, n.7, p.444-449, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Nodes from3-to 5-week-old in vitro plants of different cassava cultivars were cultured for 2-3 days on solid Murashige and Skoog basal medium supplemented with cytokinin to induce the enlargement of axillary buds. Subculture of these buds on the same medium resulted in multiple shoot formation within 4-6 weeks. Of the four cytokinins tested(6-benzylaminopurine (BAP), thidiazuron (TDZ), zeatin, and kinetin), BAP induced shoot development most efficiently. The best results were obtained with cultivar, TMS 30555, in which 63% of the explants each produced at least 25 shoots on medium with 10 mg/1 BAP. In cultivars that did not produce shoots, the addition of the surfactant Pluronic F-68 (2% wt/vol) raised the percentage of explants forming at least 5 shoots from 0 to 20-60%. Axillary buds were also used to dissect merisstems and test their ability to regenerate into shoots. Shoot formation from meristems of six different cultivars was observed alfter preculture on medium with 5 mg/1 BAP followed by transfer to mg/1 BAP. |
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Palavras-Chave: |
Gemas multiplas; Multiple shoots. |
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Thesagro: |
Explante; Mandioca; Manihot Esculenta; Micropropagação. |
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Thesaurus Nal: |
cassava; micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01758naa a2200265 a 4500
001 1079120
005 1998-03-09
008 1997 bl --- 0-- u #d
100 1 $aKONAN, N. K.
245 $aAn efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants.
260 $c1997
520 $aNodes from3-to 5-week-old in vitro plants of different cassava cultivars were cultured for 2-3 days on solid Murashige and Skoog basal medium supplemented with cytokinin to induce the enlargement of axillary buds. Subculture of these buds on the same medium resulted in multiple shoot formation within 4-6 weeks. Of the four cytokinins tested(6-benzylaminopurine (BAP), thidiazuron (TDZ), zeatin, and kinetin), BAP induced shoot development most efficiently. The best results were obtained with cultivar, TMS 30555, in which 63% of the explants each produced at least 25 shoots on medium with 10 mg/1 BAP. In cultivars that did not produce shoots, the addition of the surfactant Pluronic F-68 (2% wt/vol) raised the percentage of explants forming at least 5 shoots from 0 to 20-60%. Axillary buds were also used to dissect merisstems and test their ability to regenerate into shoots. Shoot formation from meristems of six different cultivars was observed alfter preculture on medium with 5 mg/1 BAP followed by transfer to mg/1 BAP.
650 $acassava
650 $amicropropagation
650 $aExplante
650 $aMandioca
650 $aManihot Esculenta
650 $aMicropropagação
653 $aGemas multiplas
653 $aMultiple shoots
700 1 $aSCHOPKE, C.
700 1 $aCARCAMO, R.
700 1 $aBEACHY, R. N.
700 1 $aFAUQUET, C.
773 $tPlant Cell Reports$gv.16, n.7, p.444-449, 1997.
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Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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