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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
02/05/1995 |
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Data da última atualização: |
02/05/1995 |
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Autoria: |
WANG, C. H.; BAU, H. J.; YEH, S. D. |
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Afiliação: |
Department of Plant Pathology, National Chung Hsing, University, Taichung, Taiwan, R.O.C. |
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Título: |
Comparison of the nuclear inclusion b protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
The American Phytopathological Society v. 84, n. 10, 1994. |
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ISSN: |
0331-949X |
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Idioma: |
Inglês |
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Conteúdo: |
A complementary DNA library was construced to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residues and represented part of the nuclear inclusion a (NIa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the nonpapaya-infecting Florida strain, PRSV W(fl) (W(fl)), and the nonpapaya- infecting Australia strain PRSV W(aust)). The NIb region had 83.1% nucleotide identy and 95.7% amino acid identity compared to that of HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similiar trend also was found whwen the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1 and PRSV W(fl), with 84.7% and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acididentily (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble basesand, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation. MenosA complementary DNA library was construced to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residues and represented part of the nuclear inclusion a (NIa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the nonpapaya-infecting Florida strain, PRSV W(fl) (W(fl)), and the nonpapaya- infecting Australia strain PRSV W(aust)). The NIb region had 83.1% nucleotide identy and 95.7% amino acid identity compared to that of HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similiar trend also was found whwen the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1 and PRSV W(fl), with 84.7% and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acididentily (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble basesand, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the sa... Mostrar Tudo |
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Palavras-Chave: |
Amino acid sequence. |
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Thesaurus Nal: |
Potyvirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02100naa a2200181 a 4500
001 1633151
005 1995-05-02
008 1994 bl uuuu u00u1 u #d
022 $a0331-949X
100 1 $aWANG, C. H.
245 $aComparison of the nuclear inclusion b protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity
260 $c1994
520 $aA complementary DNA library was construced to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residues and represented part of the nuclear inclusion a (NIa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the nonpapaya-infecting Florida strain, PRSV W(fl) (W(fl)), and the nonpapaya- infecting Australia strain PRSV W(aust)). The NIb region had 83.1% nucleotide identy and 95.7% amino acid identity compared to that of HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similiar trend also was found whwen the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1 and PRSV W(fl), with 84.7% and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acididentily (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble basesand, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation.
650 $aPotyvirus
653 $aAmino acid sequence
700 1 $aBAU, H. J.
700 1 $aYEH, S. D.
773 $tThe American Phytopathological Society$gv. 84, n. 10, 1994.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
25/01/2017 |
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Data da última atualização: |
25/01/2017 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
SCHMIDT, G. S. |
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Afiliação: |
GILBERTO SILBER SCHMIDT, CNPSA. |
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Título: |
Razões para as empresas avícolas não utilizarem hormônios. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Em Foco, Concórdia, ed. 75, ano 5, p. 14-15, 2016. |
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Idioma: |
Português |
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Palavras-Chave: |
Hormone. |
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Thesagro: |
Avicultura; Hormônio; Indústria agrícola; Produção animal. |
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Thesaurus NAL: |
Agricultural industry; Poultry production. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00551nam a2200181 a 4500
001 2061706
005 2017-01-25
008 2016 bl uuuu u00u1 u #d
100 1 $aSCHMIDT, G. S.
245 $aRazões para as empresas avícolas não utilizarem hormônios.$h[electronic resource]
260 $aRevista Em Foco, Concórdia, ed. 75, ano 5, p. 14-15$c2016
650 $aAgricultural industry
650 $aPoultry production
650 $aAvicultura
650 $aHormônio
650 $aIndústria agrícola
650 $aProdução animal
653 $aHormone
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Embrapa Suínos e Aves (CNPSA) |
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