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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
Gláucia Mota Bragança, Universidade Federal Fluminense (UFF) - Rio de Janeiro, RJ; Ribrio Ivan Tavares Pereira Batista; Joanna Maria Gonçalves de Souza Fabjan; Vivian Angélico Pereira Alfradique; Luana Rangel Côrtes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandao. |
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Título: |
FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. |
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Conteúdo: |
Ovarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. MenosOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified in... Mostrar Tudo |
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Palavras-Chave: |
Embryo Technology; Expressão gênica; FSH; In vitro embryo production; Produção de embriões in vitro. |
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Thesagro: |
Ovino; Reprodução animal; Transferência de embrião. |
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Thesaurus Nal: |
Gene expression; Ova; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162972/1/cnpc-2017-FSH.pdf
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Marc: |
LEADER 03935nam a2200349 a 4500
001 2074359
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aBRAGANÇA, G. M.
245 $aFSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.
520 $aOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence.
650 $aGene expression
650 $aOva
650 $aReproduction
650 $aSheep
650 $aOvino
650 $aReprodução animal
650 $aTransferência de embrião
653 $aEmbryo Technology
653 $aExpressão gênica
653 $aFSH
653 $aIn vitro embryo production
653 $aProdução de embriões in vitro
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA FABJAN, J. M. G. de
700 1 $aALFRADIQUE, V. A. P.
700 1 $aCORTÊS, L. R.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
13/01/2020 |
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Data da última atualização: |
23/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIMA, C. L. R. de; DUPONT, P. B.; PILLON, C. N.; MIOLA, E. C. C. |
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Afiliação: |
CLÁUDIA LIANE RODRIGUES DE LIMA, UFPEL; PATRÍCIA BIANCA DUPONT, UFPEL; CLENIO NAILTO PILLON, CPACT; EZEQUIEL CESAR CARVALHO MIOLA, FURG. |
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Título: |
Least limiting water range, S-index and compressibility of a Udalf under different management systems. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientia Agricola, v. 77, n. 1, e20170224, 2020. |
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Páginas: |
8 p. |
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ISSN: |
1678-992X |
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DOI: |
http://dx.doi.org/10.1590/1678-992X-2017-0224 |
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Idioma: |
Inglês |
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Conteúdo: |
Agricultural areas in the region of Turuçu, on the Southeast Hillside in the state of Rio Grande do Sul (RS), Brazil present many examples of soil physical degradation. Accord-ingly, strategies aimed at evaluating and ameliorating the structural quality of soils should be developed to ensure the sustainable use of these areas. This study aimed to evaluate the least limiting water range (LLWR), S-index, compressibility parameters, organic carbon and the organic matter granulometric fractions of a Udalf under different agricultural uses. Soil samples with disturbed and non-disturbed structure from the 0.00 to 0.10 m layer were collected on farms in the Southeast Hillside, Turuçu region, RS, for analyzing soil under: i) conventional system of corn after tobacco crop, (CT); ii) conventional system of corn after native field (CNF), iii) native field (NF) and iv) native area (NA). Both the CT and the CNF systems had a negative influence on the values of bulk density, soil porosity, least limiting water range, compressibility parameters and total organic carbon, coarse fraction carbon and carbon associated with minerals. |
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Palavras-Chave: |
Faixa de água menos limitante; Least limiting water range; LLWR. |
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Thesagro: |
Física do Solo; Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208562/1/Artigo-Claudia-Pillon-2019.pdf
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Marc: |
LEADER 01904naa a2200253 a 4500
001 2118599
005 2020-12-23
008 2020 bl uuuu u00u1 u #d
022 $a1678-992X
024 7 $ahttp://dx.doi.org/10.1590/1678-992X-2017-0224$2DOI
100 1 $aLIMA, C. L. R. de
245 $aLeast limiting water range, S-index and compressibility of a Udalf under different management systems.$h[electronic resource]
260 $c2020
300 $a8 p.
520 $aAgricultural areas in the region of Turuçu, on the Southeast Hillside in the state of Rio Grande do Sul (RS), Brazil present many examples of soil physical degradation. Accord-ingly, strategies aimed at evaluating and ameliorating the structural quality of soils should be developed to ensure the sustainable use of these areas. This study aimed to evaluate the least limiting water range (LLWR), S-index, compressibility parameters, organic carbon and the organic matter granulometric fractions of a Udalf under different agricultural uses. Soil samples with disturbed and non-disturbed structure from the 0.00 to 0.10 m layer were collected on farms in the Southeast Hillside, Turuçu region, RS, for analyzing soil under: i) conventional system of corn after tobacco crop, (CT); ii) conventional system of corn after native field (CNF), iii) native field (NF) and iv) native area (NA). Both the CT and the CNF systems had a negative influence on the values of bulk density, soil porosity, least limiting water range, compressibility parameters and total organic carbon, coarse fraction carbon and carbon associated with minerals.
650 $aFísica do Solo
650 $aSolo
653 $aFaixa de água menos limitante
653 $aLeast limiting water range
653 $aLLWR
700 1 $aDUPONT, P. B.
700 1 $aPILLON, C. N.
700 1 $aMIOLA, E. C. C.
773 $tScientia Agricola$gv. 77, n. 1, e20170224, 2020.
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Embrapa Clima Temperado (CPACT) |
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