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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
24/07/2007 |
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Data da última atualização: |
25/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARCELLOS, F. G.; MENNA, P.; BATISTA, J. S. da S.; HUNGRIA, M. |
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Afiliação: |
FERNANDO GOMES BARCELLOS; PÂMELA MENNA, UEL; JESIANE STEFÂNIA DA SILVA BATISTA†, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Evidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a brazilian savannah soil. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 73, n. 8, p. 2635-2643, Apr. 2007. |
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DOI: |
10.1128/AEM.01823-06 |
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Idioma: |
Inglês |
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Conteúdo: |
The importance of horizontal gene transfer (HGT) in the evolution and speciation of bacteria has been emphasized; however, most studies have focused on genes clustered in pathogenesis and very few on symbiosis islands. Both soybean (Glycine max [L.] Merrill) and compatible Bradyrhizobium japonicum and Bradyrhizobium elkanii strains are exotic to Brazil and have been massively introduced in the country since the early 1960s, occupying today about 45% of the cropped land. For the past 10 years, our group has obtained several isolates showing high diversity in morphological, physiological, genetic, and symbiotic properties in relation to the putative parental inoculant strains. In this study, parental strains and putative natural variants isolated from field-grown soybean nodules were genetically characterized in relation to conserved genes (by repetitive extragenic palindromic PCR using REP and BOX A1R primers, PCR-restriction fragment length polymorphism, and sequencing of the 16SrRNA genes), nodulation, and N2-fixation genes (PCR-RFLP and sequencing of nodY-nodA, nodC, and nifH genes). Both genetic variability due to adaptation to the stressful environmental conditions of the Brazilian Cerrados and HGT events were confirmed. One strain (S 127) was identified as an indigenous B. elkanii strain that acquired a nodC gene from the inoculant B. japonicum. Another one (CPAC 402) was identified as an indigenous Sinorhizobium (Ensifer) fredii strain that received the whole symbiotic island from the B. japonicum inoculant strain and maintained an extra copy of the original nifH gene. The results highlight the strategies that bacteria may commonly use to obtain ecological advantages, such as the acquisition of genes to establish effective symbioses with an exotic host legume. MenosThe importance of horizontal gene transfer (HGT) in the evolution and speciation of bacteria has been emphasized; however, most studies have focused on genes clustered in pathogenesis and very few on symbiosis islands. Both soybean (Glycine max [L.] Merrill) and compatible Bradyrhizobium japonicum and Bradyrhizobium elkanii strains are exotic to Brazil and have been massively introduced in the country since the early 1960s, occupying today about 45% of the cropped land. For the past 10 years, our group has obtained several isolates showing high diversity in morphological, physiological, genetic, and symbiotic properties in relation to the putative parental inoculant strains. In this study, parental strains and putative natural variants isolated from field-grown soybean nodules were genetically characterized in relation to conserved genes (by repetitive extragenic palindromic PCR using REP and BOX A1R primers, PCR-restriction fragment length polymorphism, and sequencing of the 16SrRNA genes), nodulation, and N2-fixation genes (PCR-RFLP and sequencing of nodY-nodA, nodC, and nifH genes). Both genetic variability due to adaptation to the stressful environmental conditions of the Brazilian Cerrados and HGT events were confirmed. One strain (S 127) was identified as an indigenous B. elkanii strain that acquired a nodC gene from the inoculant B. japonicum. Another one (CPAC 402) was identified as an indigenous Sinorhizobium (Ensifer) fredii strain that received the whole symbiotic... Mostrar Tudo |
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Thesagro: |
Fixação de Nitrogênio; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02508naa a2200193 a 4500
001 1470103
005 2017-07-25
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1128/AEM.01823-06$2DOI
100 1 $aBARCELLOS, F. G.
245 $aEvidence of horizontal transfer of symbiotic genes from a Bradyrhizobium japonicum inoculant strain to indigenous diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a brazilian savannah soil.
260 $c2007
520 $aThe importance of horizontal gene transfer (HGT) in the evolution and speciation of bacteria has been emphasized; however, most studies have focused on genes clustered in pathogenesis and very few on symbiosis islands. Both soybean (Glycine max [L.] Merrill) and compatible Bradyrhizobium japonicum and Bradyrhizobium elkanii strains are exotic to Brazil and have been massively introduced in the country since the early 1960s, occupying today about 45% of the cropped land. For the past 10 years, our group has obtained several isolates showing high diversity in morphological, physiological, genetic, and symbiotic properties in relation to the putative parental inoculant strains. In this study, parental strains and putative natural variants isolated from field-grown soybean nodules were genetically characterized in relation to conserved genes (by repetitive extragenic palindromic PCR using REP and BOX A1R primers, PCR-restriction fragment length polymorphism, and sequencing of the 16SrRNA genes), nodulation, and N2-fixation genes (PCR-RFLP and sequencing of nodY-nodA, nodC, and nifH genes). Both genetic variability due to adaptation to the stressful environmental conditions of the Brazilian Cerrados and HGT events were confirmed. One strain (S 127) was identified as an indigenous B. elkanii strain that acquired a nodC gene from the inoculant B. japonicum. Another one (CPAC 402) was identified as an indigenous Sinorhizobium (Ensifer) fredii strain that received the whole symbiotic island from the B. japonicum inoculant strain and maintained an extra copy of the original nifH gene. The results highlight the strategies that bacteria may commonly use to obtain ecological advantages, such as the acquisition of genes to establish effective symbioses with an exotic host legume.
650 $aFixação de Nitrogênio
650 $aSoja
700 1 $aMENNA, P.
700 1 $aBATISTA, J. S. da S.
700 1 $aHUNGRIA, M.
773 $tApplied and Environmental Microbiology$gv. 73, n. 8, p. 2635-2643, Apr. 2007.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 9 | |
| 1. |  | CARVALHO, G. M. C.; BEFFA, L. M.; ALMEIDA, M. J. O.; SILVA, L. R. F. da. Caracterização fenotípica de três rebanhos bovinos da raça Pé-duro em ambientes distintos do Piauí. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 504| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | CARVALHO, G. M. C.; MACIEL, G. A.; ALMEIDA, M. J. de O.; SILVA, L. R. F. da. Avaliação de características fenotípicas de pelagens em bovinos da raça Pé-Duro (Bos taurus taurus). In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Ref. Animais. p. 15 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 3. | | CARVALHO, G. M. C.; AZEVEDO, D. M. M. R.; ALMEIDA, M. J. de O.; SILVA, L. R. F. da. Avaliação do desenvolvimento ponderal de bovinos da raça Pé-Duro (Bos taurus taurus), Nelore (Bos taurus indicus) e de seus mestiços F1. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Ref. Animais. p. 50 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | SILVA, L. R. F. da; ALVES, A. A.; VASCONCELOS, V. R.; NASCIMENTO, H. T. S. do; MOREIRA FILHO, M. A. Nutritive value of diets containing pods of faveira (Parkia platycephala Benth.) for confined finishing sheep. Revista Brasileira de Zootecnia, Viçosa, MG, v. 41, n. 4, p. 1065-1069, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 5. | | CARVALHO, G. M. C.; ALMEIDA, M. J. de O.; LEAL, J. A.; SALES, F. S. M.; SILVA, L. R. F. da. Características fenotípicas del ganado curraleiro del Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 5., 2005, Montevideo, Uruguay. Resúmenes... Montevideo: Instituto Nacional de Investigación Agropecuaria: Universidad de la República, Facultad de Agronomía, 2005. n. 311, p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 6. | | AZAR, G. S.; COSTA, J. V.; SILVA, L. R. F. da; RODRIGUES, M. M.; OLIVEIRA, M. E. de; AZEVEDO, D. M. M. R. Características do pasto de capim-marandu irrigado sob sistemas de monocultura e silvipastoril em duas condições de pastejo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 7. | | ALVES, A. A.; SALES, R. de O.; NEIVA, J. N. M.; MEDEIROS, A. N.; BRAGA, A. P.; AZEVEDO, D. M. M. R.; SILVA, L. R. F. da. Metabolismo de compostos nitrogenados em ovinos alimentados com dietas contendo vagens de faveira. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 12, n. 4, p. 1051-1066, out./dez 2011. 1519-9940| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 8. | | LOPES, L. D; SILVA, L. R. F, da; ROMAGNOLI, E. M; FERREIRA, C.; TAKETANI, R. G.; ABDALLA, A. L.; LOUVANDINI, H.; MENDES, R. Metagenomics of sheep rumen microbiome under two diet. XXI ALAM Congresso Latinoamericano de Microbiologia, Santos-Brasil 28/10/12 a 01/11/2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 9. | | ROMAGNOLI, E. M; LOPES, L. D.; SILVA, L. R. F, da; NAKAMURA, F. M; SBARDELLA, M.; PEREIRA, R.; SILVA, W. D.; TSAI, S. M.; ANDREOTE, F. D.; MENDES, R. Quantification of Archaea and bacteria domains in the sheep rumen incubated under two diet conditions. The International Sodety for Microblal EcoJogy(ISME), organlzer of the 14th International SympoSium on Microblal Eco!ogy (ISME14 )in Copenhagen, Denmark, from 19 - 24 August 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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