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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/10/1995 |
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Data da última atualização: |
31/05/2023 |
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Autoria: |
HU, J. S.; LI, H. P.; BARRY, K.; WANG, M. |
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Afiliação: |
Department of Plant Pathology, University of Hawaii, Honolulu 96822; and R.Jordan, USDA-ARS, U.S. National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, Md. 20705. |
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Título: |
Comparison of dot blot, ELISA, and RT-PCR Assays detection of two cucumber mosaic virus isolates infecting banana in Hawaii. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Plant Disease, v.79, n.9, 1995. |
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ISSN: |
0191-2917 |
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Idioma: |
Inglês |
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Conteúdo: |
The coat protein genes of cucumber mosaic virus (CMV) isolates infecting banana plants in Hawaii were cloned and sequenced. Based on nucleotide and amino acid sequence comparisons, both isolates belong to CMV subgroup I. One isolate (CMV-Hawaii), wich is common in banana plants in the state of Hawaii and induces mild mosaic symptoms, shares 99% sequence identity (both nucleotid and amino acid sequences) with CMV-C strain. Another isolate (CMV-Oahu), which was found only at two banana farms in the state of Hawaii, induces severe mosaic and leaf distrortion symptoms. CMV-Oahu 91% nucleotide and amino acid sequence identity, respectively, with CMV-C and CMV-Hawaii. A reserve transcription-polymerase chain reaction (RT-PCR) assay was developed for detection of both CMV subgroups from banana samples. The RT-PCR product (~750 bp) was also labeled as a probe to detect CMV in dot blot hybridization tests.PCR is a more sensitive assay than either dot blot or ELISA. The dot blot assay was 100 times more sensitive than ELISA. The distribution ofCMV within banana plants was uneven. CMV concentrations were higher in leaves than in older ones. The CMV-Oahu isolate was not detected in banana by ELISA using antibodies to both CMV subgroups I and II, but was positive in dot blot and RT-PCR tests. |
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Palavras-Chave: |
Detectacao de virus. |
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Thesagro: |
Doença; Gene. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01882naa a2200205 a 4500
001 1634052
005 2023-05-31
008 1995 bl uuuu u00u1 u #d
022 $a0191-2917
100 1 $aHU, J. S.
245 $aComparison of dot blot, ELISA, and RT-PCR Assays detection of two cucumber mosaic virus isolates infecting banana in Hawaii.$h[electronic resource]
260 $c1995
520 $aThe coat protein genes of cucumber mosaic virus (CMV) isolates infecting banana plants in Hawaii were cloned and sequenced. Based on nucleotide and amino acid sequence comparisons, both isolates belong to CMV subgroup I. One isolate (CMV-Hawaii), wich is common in banana plants in the state of Hawaii and induces mild mosaic symptoms, shares 99% sequence identity (both nucleotid and amino acid sequences) with CMV-C strain. Another isolate (CMV-Oahu), which was found only at two banana farms in the state of Hawaii, induces severe mosaic and leaf distrortion symptoms. CMV-Oahu 91% nucleotide and amino acid sequence identity, respectively, with CMV-C and CMV-Hawaii. A reserve transcription-polymerase chain reaction (RT-PCR) assay was developed for detection of both CMV subgroups from banana samples. The RT-PCR product (~750 bp) was also labeled as a probe to detect CMV in dot blot hybridization tests.PCR is a more sensitive assay than either dot blot or ELISA. The dot blot assay was 100 times more sensitive than ELISA. The distribution ofCMV within banana plants was uneven. CMV concentrations were higher in leaves than in older ones. The CMV-Oahu isolate was not detected in banana by ELISA using antibodies to both CMV subgroups I and II, but was positive in dot blot and RT-PCR tests.
650 $aDoença
650 $aGene
653 $aDetectacao de virus
700 1 $aLI, H. P.
700 1 $aBARRY, K.
700 1 $aWANG, M.
773 $tPlant Disease$gv.79, n.9, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 8 | |
| 1. |  | MYBURG, A. A.; GRATTAPAGLIA, D.; TUSKAN, G. A.; SCHMUTZ, J.; BARRY, K.; BRISTOW, J. D. Sequencing the Eucalyptus genome: genome resources for renewable energy and fiber production. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, w195.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | MAYBURG, A. A.; GRATTAPAGLIA, D.; TUSKAN, G. A.; SCHMUTZ, J.; ROKHSAR, D. S.; ROTTMANN, W. H.; HINCHEE, M. A.; BARRY, K.; BRISTOW, J. The eucalyptus genome project: a second forest tree genome for renewable energy and fiber production. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 17., 2009, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2009. W178| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | BUTLER, J. B.; FREEMAN, J. S.; POTTS, B. M.; VAILLANCOURT, R. E.; GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; SIMMONS, B. A.; HEALEY, A. L.; SCHMUTZ, J.; BARRY, K. W.; LEE, D. J.; HENRY, R. J.; KING, G. J.; BATEN, A.; SHEPHERD, M. Annotation of the Corymbia terpene synthase gene family shows broad conservation but dynamic evolution of physical clusters relative to Eucalyptus. Heredity, v. 121, n. 1, p. 87-104, 2018. Na publicação: Orzenil B. Silva-Junior.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | BUTLER, J. B.; FREEMAN, J. S.; POTTS, B. M.; VAILLANCOURT, R. E.; GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; SIMMONS, B.; SCHMUTZ, J.; BARRY, K. W.; LEE, D. J.; HEALEY, A.; FURTADO, A.; HENRY, R. J.; BATEN, A.; KING, G.; SHEPHERD, M. Comparative analysis of the terpene synthase gene family between Eucalyptus and Corymbia. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. P0598.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | HEALEY, A.; SHEPHERD, M.; BATEN, A.; KING, G. J.; LEE, D. J.; FURTADO, A.; VAILLANCOURT, R. E.; BUTLER, J. B.; FREEMAN, J. S.; POTTS, B. M.; GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; BARRY, K. W.; SCHMUTZ, J.; SIMMONS, B.; HENRY, R. J. Sequencing the branches of the eucalypt tree: comparison between Eucalyptus and Corymbia genomes. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. W822.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | HEALEY, A. L.; SHEPHERD, M.; KING, G. J.; BUTLER, J. B.; FREEMAN, J. S.; LEE, D. J.; POTTS, B. M.; SILVA JUNIOR, O. B. da; BATEN, A.; JENKINS, J.; SHU, S.; LOVELL, J. T.; SREEDASYAM, A.; GRIMWOOD, J.; FURTADO, A.; GRATTAPAGLIA, D.; BARRY, K. W.; HUNDLEY, H.; SIMMONS, B. A.; SCHMUTZ, J.; VAILLANCOURT, R. E.; HENRY, R. J. Pests, diseases, and aridity have shaped the genome of Corymbia citriodora. Communications Biology, v. 4, 537, 2021. Na publicação: Orzenil B. Silva-Junior.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | SREEDASYAM, A.; PLOTT, C.; HOSSAIN, M. S.; LOVELL, J. T.; GRIMWOOD, J.; JENKINS, J. W.; DAUM, C.; BARRY, K.; CARLSON, J.; SHU, S.; PHILLIPS, J.; AMIREBRAHIMI, M.; ZANE, M.; WANG, M.; GOODSTEIN, D.; HAAS, F. B.; HISS, M.; PERROUD, P.-F.; JAWDY, S. S.; YANG, Y.; HU, R.; JOHNSON, J.; KROPAT, J.; GALLAHER, S. D.; LIPZEN, A.; SHAKIROV, E. V.; WENG, X.; TORRES-JEREZ, I.; WEERS, B.; CONDE, D.; PAPPAS, M. de C. R.; LIU, L.; MUCHLINSKI, A.; JIANG, H.; SHYU, C.; HUANG, P.; SEBASTIAN, J.; LAIBEN, C.; MEDLIN, A.; CAREY, S.; CARRELL, A. A.; CHEN, J.-G.; PERALES, M.; SWAMINATHAN, K.; ALLONA, I.; GRATTAPAGLIA, D.; COOPER, E. A.; THOLL, D.; VOGEL, V. P.; WESTON, D. J.; YANG, X.; BRUTNELL, T. P.; KELLOGG, E. A.; BAXTER, I.; UDVARDI, M.; TANG, Y.; MOCKLER, T. C.; JUENGER, T. E.; MULLET, J.; RENSING, S. A.; TUSKAN, G. A.; MERCHANT, S. S.; STACEY, G.; SCHMUTZ, J. JGI Plant Gene Atlas: an updateable transcriptome resource to improve functional gene descriptions across the plant kingdom. Nucleic Acids Research, v. 51, n. 16, p. 8383-8401, 2023. Na publicação: Marilia R. Pappas.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | MYBURG, A. A.; GRATTAPAGLIA, D.; TUSKAN, G. A.; HELLSTEN, U.; HAYES, R. D.; GRIMWOOD, J.; JENKINS, J.; LINDQUIST, E.; BAUER, D.; GOODSTEIN, D. M.; DUBCHAK, I.; POLIAKOV, A.; MIZRACHI, E.; KULLAN, A. R. K.; HUSSEY, S. G.; PINARD, D.; MERWE, K. van der; SINGH, P.; JAARSVELD, I. van; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS, M. R.; FARIA, D. A.; SANSALONI, C. P.; PETROLI, C. D.; YANG, X.; RANJAN, P.; TSCHAPLINSKI, T. J.; YE, C.-Y.; LI, T.; STERCK, L.; VANNESTE, K.; MURAT, F.; SOLER, M.; SAN CLEMENTE, H.; SAIDI, N.; CASSAN-WANG, H.; DUNAND, C.; HEFER, C. A.; BORNBERG-BAUER, E.; KERSTING, A. R.; VINING, K.; AMARASINGHE, V.; RANIK, M.; NAITHANI, S.; ELSER, J.; BOYD, A. E.; LISTON, A.; SPATAFORA, J. W.; DHARMWARDHANA, P.; RAJA, R.; SULLIVAN, C.; ROMANEL, E.; ALVES-FERREIRA, M.; KULHEIM, C.; FOLEY, W.; CAROCHA, V.; PAIVA, J.; KUDRNA, D.; BROMMONSCHENKEL, S. H.; PASQUALI, G.; BYRNE, M.; RIGAULT, P.; SPOKEVICIUS, A.; JONES, R. C.; STEANE, D. A.; VAILLANCOURT, R. E.; POTTS, B. M.; JOUBERT, F.; BARRY, K.; PAPPAS JUNIOR, G. J.; STRAUSS, S. H.; JAISWAL, P.; GRIMA-PETTENATI, J.; SALSE, J.; PEER, Y. van de; ROKHSAR, D. S.; SCHMUTZ, J. The genome of Eucalyptus grandis. Nature (London), v. 510, p. 356-362, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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