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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Territorial. |
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Data corrente: |
20/07/2022 |
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Data da última atualização: |
04/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HOFFMANN, L. V.; BRANQUINHO, A. A.; BARROSO, P. A. V.; VASLIN, M. F. S. |
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Afiliação: |
LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, article 814119, 5 p. 2022. |
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ISBN: |
1664-462X |
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DOI: |
10.3389/fpls.2022.814119 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
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Palavras-Chave: |
CLRDV; Enzyme-linked immunosorbent assay (ELISA); Intermediary host. |
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Thesagro: |
Vírus. |
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Thesaurus Nal: |
Serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144832/1/6046.pdf
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Marc: |
LEADER 01770naa a2200241 a 4500
001 2151489
005 2023-05-04
008 2022 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2022.814119$2DOI
100 1 $aHOFFMANN, L. V.
245 $aAntibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.$h[electronic resource]
260 $c2022
520 $aThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.
650 $aSerology
650 $aVírus
653 $aCLRDV
653 $aEnzyme-linked immunosorbent assay (ELISA)
653 $aIntermediary host
700 1 $aBRANQUINHO, A. A.
700 1 $aBARROSO, P. A. V.
700 1 $aVASLIN, M. F. S.
773 $tFrontiers in Plant Science$gv. 13, article 814119, 5 p. 2022.
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Registro original: |
Embrapa Algodão (CNPA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2022 |
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Data da última atualização: |
12/12/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SALGADO, F. F.; VIEIRA, L. R.; SILVA, V, N. B.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
FERNANDA FERREIRA SALGADO, UNIVERSIDADE FEDERAL DE LAVRAS; LETÍCIA RIOS VIEIRA, UNIVERSIDADE FEDERAL DE LAVRAS; VIVIANNY NAYSE BELO SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; ANDRE PEREIRA LEAO, CNPAE; PRISCILA GRYNBERG, Cenargen; MARCOS MOTA DO CARMO COSTA, Cenargen; ROBERTO COITI TOGAWA, Cenargen; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Prediction and identification of miRNAS in Elaeis Guinensis Jacq and analysis of their expression in oil palm plants under salinity drought stress. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. |
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Páginas: |
p. 275 |
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Idioma: |
Inglês |
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Conteúdo: |
Oil palm (Elaeis guineensis Jacq.) is an oilseed crop of great economic importance. The oil palm industry has a large-scale production worldwide and uses high efficient extraction and refining processes to obtain palm oil and palm kernel oil. In Brazil, currently, there is an extensive area suitable for oil palm planting outside the Amazon rainforest; however, these areas go through long periods of water scarcity and demand artificial irrigation to be sustainable. One-quarter of the irrigated area in agriculture has a problem with salinity stress. |
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Thesaurus NAL: |
Abiotic stress; Non-coding RNA; Oil palm products; Transcription factors. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01402nam a2200265 a 4500
001 2149446
005 2022-12-12
008 2022 bl uuuu u01u1 u #d
100 1 $aSALGADO, F. F.
245 $aPrediction and identification of miRNAS in Elaeis Guinensis Jacq and analysis of their expression in oil palm plants under salinity drought stress.$h[electronic resource]
260 $aIn: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN.$c2022
300 $ap. 275
520 $aOil palm (Elaeis guineensis Jacq.) is an oilseed crop of great economic importance. The oil palm industry has a large-scale production worldwide and uses high efficient extraction and refining processes to obtain palm oil and palm kernel oil. In Brazil, currently, there is an extensive area suitable for oil palm planting outside the Amazon rainforest; however, these areas go through long periods of water scarcity and demand artificial irrigation to be sustainable. One-quarter of the irrigated area in agriculture has a problem with salinity stress.
650 $aAbiotic stress
650 $aNon-coding RNA
650 $aOil palm products
650 $aTranscription factors
700 1 $aVIEIRA, L. R.
700 1 $aSILVA, V, N. B.
700 1 $aLEAO, A. P.
700 1 $aGRYNBERG, P.
700 1 $aCOSTA, M. M. do C.
700 1 $aTOGAWA, R. C.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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