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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Instrumentação. |
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Data corrente: |
18/11/2021 |
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Data da última atualização: |
23/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. |
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Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ALICE KAZUKO INOUE NAGATA, CNPH; ERICH YUKIO TEMPEL NAKASU, CNPH; LUCIMEIRE PILON, CNPH. |
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Título: |
Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. |
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Páginas: |
5266 - 5279 |
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DOI: |
https://doi.org/10.33263/BRIAC124.52665279 |
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Idioma: |
Inglês |
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Conteúdo: |
Technologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection. |
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Palavras-Chave: |
Chitosan nanocarrier; DsRNA phytotoxicity assay; Tomato mosaic virus (ToMV). |
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Thesaurus Nal: |
Double-stranded RNA; Lettuce; RNA interference; Tomato mosaic virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232647/1/P-Physicochemical-and-Toxicity-Investigation-of-Chitosanbased-dsRNA-Nanocarrier-Formation.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227886/1/20695837124.526652791.pdf
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Marc: |
LEADER 02428naa a2200313 a 4500
001 2141015
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.33263/BRIAC124.52665279$2DOI
100 1 $aPETRÔNIO, M. S.
245 $aPhysicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation.$h[electronic resource]
260 $c2022
300 $a5266 - 5279
520 $aTechnologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection.
650 $aDouble-stranded RNA
650 $aLettuce
650 $aRNA interference
650 $aTomato mosaic virus
653 $aChitosan nanocarrier
653 $aDsRNA phytotoxicity assay
653 $aTomato mosaic virus (ToMV)
700 1 $aBARROS-ALEXANDRINO, T. T.
700 1 $aLIMA, A. M. F.
700 1 $aASSIS, O. B. G. de
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAKASU, E. Y. T.
700 1 $aTIERA, M. J.
700 1 $aPILON, L.
773 $tBiointerface Research in Applied Chemistry, vol. 12$gn. 4, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 8 | |
| 2. |  | BARROS-ALEXANDRINO, T. T.; MARTELLI-TOSI, M.; BERNARDES FILHO, R.; ASSIS, O. B. G. de. Comparação entre o uso nanopartículas de quitosana e nanofibras de celulose como material de reforço em filmes a base de polpa de frutas. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p.188-192. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 3. | | BARROS-ALEXANDRINO, T. T.; MARTELLI-TOSI, M.; ASSIS, O. B. G. de. Efeito da adição de pectina nas propriedades mecânicas e de permeação ao vapor de água de filmes a base de purê de mamão. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p.192-195. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | CUNHA, D. A. L. V.; BARROS-ALEXANDRINO, T. T.; ASSIS, O. B. G. de. Otimização do processo de obtenção de nanopartículas de quitosana-TPP pelo método de gelificação ionotrópica. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 56-59. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | BARROS-ALEXANDRINO, T. T.; MARTELLI-TOSI, M.; ASSIS, O. B. G. de. Síntese e caracterização de nanopartículas de quitosana-TPPP como inibidoras do crescimento fúngico. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 161-164. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | RIBEIRO, E. F.; BARROS-ALEXANDRINO, T. T.; ASSIS, O. B. G. de; CRUZ JUNIOR, A.; QUILES, A.; HERNANDO, I.; NICOLETTI, V. R. Chitosan and crosslinked chitosan nanoparticles: Synthesis, characterization and their role as Pickering emulsifiers. Carbohydrate Polymers, v. 250, 116878, 2020. 1 - 10| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 8. | | PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. 5266 - 5279| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Instrumentação. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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