Data/hora: 21/06/2024 03:42:02

Biblioteca(s): | Embrapa Café.
Data corrente: | 15/12/2017
Data da última atualização: | 15/12/2017
Autoria: | BARKA, G. D.; CAIXETA, E. T.; ALMEIDA, R. F. de; ALVARENGA, S. M.; ZAMBOLIM, L.
Afiliação: | Geleta Dugassa Barka, BIOCAFÉ/BIOAGRO/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; Robson Ferreira de Almeida, Instituto Federal do Espírito Santo; Samuel Mazzinghy Alvarenga, FEPAGRO; Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa.
Título: | Differential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions.
Ano de publicação: | 2017
Fonte/Imprenta: | European Journal of Plant Pathology, v. 149, n. 3, p. 543-561, Nov. 2017.
Idioma: | Inglês
Conteúdo: | Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed.
Palavras-Chave: | Coffee; Plant-pathogen interaction; Rust disease.
Thesagro: | Café; Ferrugem; Patogeno.
Thesaurus Nal: | Gene expression; pest resistance.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/169104/1/Differential-expression-of-molecular-rust-resistance.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1218-1) - UPC - AP - DD -  -  -  - DIS