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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
05/04/2005 |
Data da última atualização: |
05/04/2005 |
Autoria: |
BARIL, G.; TOUZÉ, J. L.; PIGNON, R.; SAUMANDE, J. |
Título: |
Evaluation of the efficiency of transrectal ultrassound to study ovarian function in goats. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Theriogenology, v. 53, n. 1, p. 371, 2000. |
Idioma: |
Inglês |
Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. |
Conteúdo: |
In goats, laparoscopic examination of the ovaries is used to determine ovulation rate and time of ovulation but this method is stressful and is becoming less acceptable. Time of ovulation can also be evaluated by the occurrence of the LH preovulatory peak; however it is an indirect method that assumes that the LH peak-ovulation interval is constant. A more acceptable technique is ultrasonic examination of the ovaries; which has been used in goats but, to our knowledge, the accuracy has not been evaluated. In the present experiment, ultrasound and laparoscopic examination of the ovaries were compared for 2 purposes: to determine the time of ovulation (Experiment 1) and to determine the CL numbers (Experiment 2). The estrous cycles of Alpine and Saanen goats (Exp 1, n=22; Exp 2, n=116) were synchronized using the FGA, cloprostenol, eCG treatment. TransrectaI examinations of the ovaries were made on goats in a standing position using a 7.5 MHz probe and Kretz Combison 310A ultrasonography unit. Exp 1. The number and size of follicles were measured 24 h after the end of the progestagen treatment then blood samples were coIlected at 4 h intervals to determine time of the LH peak. An LH peak was detected in 20 goats and ovarian ultrasound examinations were performed on these animaIs every 4 h, beginning 18 h after the LH peak until ovulation. Ovulation was considered to have occured when follicles =3mm were no longer present; this was immediately checked by laparoscopy. Two goats with no LH peak had their ovaries checked by both methods 52 h after the end of the progestagen treatment. The correlation between the two methods was evaluated on a per ovary basis. Ovulation occured in the 20 goats where the LH peak was detected and concemed 27 ovaries. According to the sonograms, the LH peak occured for 3 goats before 18 h and between 18-22 h, 22-26 h and 26-30 h for 12,4 and 1 other goat(s), respectively. This distribution is in agreement with the data obtained by laparoscopic method (Leboeuf et al., VI Int Conf Goats, Beijing, China 1996;2 :828). The overaIl correlation between methods on ovulatory (25/27; 92.6%) or anovulatory (14/17; 82.4%) cycles was 88.6% (39/44). Exp 2. The correlation between the numbers of CL detected by ultrasound and laparoscopy was studied in 5 trials (1, n=28; 2, n=31; 3, n=17; 4, n=12; 5, n=14) on Day 6 (Day 0= estrus) and in one trial (6, n=14) on Day 10 between July, 1997 and July, 1999. Ultrasound determination was made by the same operator in the 6 trials, then laparoscopy by a different trained operator who was not aware of ultrasound data. It was possible to observe 230/232 (99.1 %) ovaries; in two goats only one ovary could be scanned. The overaIl agreement for the estimation of CL number was 75.7%. However, operator experience seemed to play an important role in the results. There was a significant effect (P<0.02) of trial number on the correlation between the methods: (1: 62.5%; 2: 77.4%; 3: 66.7%; 4: 83.3%; 5: 92.9%, respectively, for 56, 62, 33, 24, 28 ovaries observed when determinations were made on Day 6). Whereas results for trial 6 (85.2% ; 27 ovaries) were not different from trials 4 and 5, the correlation calculated for the 3 Iast trials was significantly better than the initial3 trials (87.3% vs 69.5%; P<0.003). These data indicate that uItrasonography may provide an alternative to laparoscopic examination of ovaries in goats. However, further research is needed to determine the overaII accuracy ofthis technique. From a weIfare point of view it should be chosen whenever possible. MenosIn goats, laparoscopic examination of the ovaries is used to determine ovulation rate and time of ovulation but this method is stressful and is becoming less acceptable. Time of ovulation can also be evaluated by the occurrence of the LH preovulatory peak; however it is an indirect method that assumes that the LH peak-ovulation interval is constant. A more acceptable technique is ultrasonic examination of the ovaries; which has been used in goats but, to our knowledge, the accuracy has not been evaluated. In the present experiment, ultrasound and laparoscopic examination of the ovaries were compared for 2 purposes: to determine the time of ovulation (Experiment 1) and to determine the CL numbers (Experiment 2). The estrous cycles of Alpine and Saanen goats (Exp 1, n=22; Exp 2, n=116) were synchronized using the FGA, cloprostenol, eCG treatment. TransrectaI examinations of the ovaries were made on goats in a standing position using a 7.5 MHz probe and Kretz Combison 310A ultrasonography unit. Exp 1. The number and size of follicles were measured 24 h after the end of the progestagen treatment then blood samples were coIlected at 4 h intervals to determine time of the LH peak. An LH peak was detected in 20 goats and ovarian ultrasound examinations were performed on these animaIs every 4 h, beginning 18 h after the LH peak until ovulation. Ovulation was considered to have occured when follicles =3mm were no longer present; this was immediately checked by laparoscopy. Two goat... Mostrar Tudo |
Palavras-Chave: |
Função ovariana; Ultra-som transretal. |
Thesagro: |
Caprino; Ovário; Reprodução Animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 04328naa a2200229 a 4500 001 1531043 005 2005-04-05 008 2000 bl uuuu u00u1 u #d 100 1 $aBARIL, G. 245 $aEvaluation of the efficiency of transrectal ultrassound to study ovarian function in goats. 260 $c2000 500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. 520 $aIn goats, laparoscopic examination of the ovaries is used to determine ovulation rate and time of ovulation but this method is stressful and is becoming less acceptable. Time of ovulation can also be evaluated by the occurrence of the LH preovulatory peak; however it is an indirect method that assumes that the LH peak-ovulation interval is constant. A more acceptable technique is ultrasonic examination of the ovaries; which has been used in goats but, to our knowledge, the accuracy has not been evaluated. In the present experiment, ultrasound and laparoscopic examination of the ovaries were compared for 2 purposes: to determine the time of ovulation (Experiment 1) and to determine the CL numbers (Experiment 2). The estrous cycles of Alpine and Saanen goats (Exp 1, n=22; Exp 2, n=116) were synchronized using the FGA, cloprostenol, eCG treatment. TransrectaI examinations of the ovaries were made on goats in a standing position using a 7.5 MHz probe and Kretz Combison 310A ultrasonography unit. Exp 1. The number and size of follicles were measured 24 h after the end of the progestagen treatment then blood samples were coIlected at 4 h intervals to determine time of the LH peak. An LH peak was detected in 20 goats and ovarian ultrasound examinations were performed on these animaIs every 4 h, beginning 18 h after the LH peak until ovulation. Ovulation was considered to have occured when follicles =3mm were no longer present; this was immediately checked by laparoscopy. Two goats with no LH peak had their ovaries checked by both methods 52 h after the end of the progestagen treatment. The correlation between the two methods was evaluated on a per ovary basis. Ovulation occured in the 20 goats where the LH peak was detected and concemed 27 ovaries. According to the sonograms, the LH peak occured for 3 goats before 18 h and between 18-22 h, 22-26 h and 26-30 h for 12,4 and 1 other goat(s), respectively. This distribution is in agreement with the data obtained by laparoscopic method (Leboeuf et al., VI Int Conf Goats, Beijing, China 1996;2 :828). The overaIl correlation between methods on ovulatory (25/27; 92.6%) or anovulatory (14/17; 82.4%) cycles was 88.6% (39/44). Exp 2. The correlation between the numbers of CL detected by ultrasound and laparoscopy was studied in 5 trials (1, n=28; 2, n=31; 3, n=17; 4, n=12; 5, n=14) on Day 6 (Day 0= estrus) and in one trial (6, n=14) on Day 10 between July, 1997 and July, 1999. Ultrasound determination was made by the same operator in the 6 trials, then laparoscopy by a different trained operator who was not aware of ultrasound data. It was possible to observe 230/232 (99.1 %) ovaries; in two goats only one ovary could be scanned. The overaIl agreement for the estimation of CL number was 75.7%. However, operator experience seemed to play an important role in the results. There was a significant effect (P<0.02) of trial number on the correlation between the methods: (1: 62.5%; 2: 77.4%; 3: 66.7%; 4: 83.3%; 5: 92.9%, respectively, for 56, 62, 33, 24, 28 ovaries observed when determinations were made on Day 6). Whereas results for trial 6 (85.2% ; 27 ovaries) were not different from trials 4 and 5, the correlation calculated for the 3 Iast trials was significantly better than the initial3 trials (87.3% vs 69.5%; P<0.003). These data indicate that uItrasonography may provide an alternative to laparoscopic examination of ovaries in goats. However, further research is needed to determine the overaII accuracy ofthis technique. From a weIfare point of view it should be chosen whenever possible. 650 $aCaprino 650 $aOvário 650 $aReprodução Animal 653 $aFunção ovariana 653 $aUltra-som transretal 700 1 $aTOUZÉ, J. L. 700 1 $aPIGNON, R. 700 1 $aSAUMANDE, J. 773 $tTheriogenology$gv. 53, n. 1, p. 371, 2000.
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2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FREITAS, V. J. F.; LOPES-JUNIOR, E. S.; RONDINA, D.; SALMITO-VANDERLEY, C. S. B.; SALLES, H. O.; SIMPLÍCIO, A. A.; BARIL, G.; SAUMANDE, J. Puberty in Anglo-Nubian and Saanen female kids raised in the semi-arid of North-eastern Brazil. Small Ruminant Research, v. 53, n. 1/2, p. 167-172, 2004. Technical note.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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