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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
23/10/2013 |
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Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
LIMA, N. B.; MARQUES, M. W.; MICHEREFF, S. J.; MORAIS JÚNIOR, M. A.; BARBOSA, M. A. G.; CÂMARA, M. P. S. |
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Afiliação: |
MARIA ANGELICA GUIMARAES BARBOSA, CPATSA. |
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Título: |
First report of mango anthracnose caused by Colletotrichum karstii in Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Plant Disease, v. 97, n. 9, p. 1248, sept. 2013. |
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DOI: |
10.1094/PDIS-01-13-0002-PDN |
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Idioma: |
Inglês |
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Conteúdo: |
From April to June 2010, mango fruits (Mangifera indica L.) (cv. Tommy Atkins) showing post-harvest anthracnose symptoms were collected during a survey conducted in São Francisco Valley, northeastern Brazil. Fruits affected by anthracnose showed sunken, prominent, dark brown to black decay spots. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g liter–1 streptomycin sulfate. Plates were incubated at 25°C in the dark for 5 to 7 days and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA (1). Identification was made using morphological characteristics and phylogenetic analysis. Two isolates (CMM 4101 and CMM 4102) presented colonies that had white aerial mycelia and orange conidial mass, varying between colorless and pale orange in reverse. Conidia were hyaline, cylindrical, and aseptate 14.52 (10.40 to 20.20) μm long and 4.90 (3.80 to 6.50) μm wide, length/width ratio = 3.0. Mycelial growth rate was 5.20 mm per day at 25°C. Morphological and cultural characterizations were consistent with the description of Colletotrichum karstii (3). PCR amplification by universal primers (ITS1/ITS4) and DNA sequencing of the internal transcribed spacer (ITS1-5.8S-ITS2 rRNA gene cluster) were conducted to confirm the identifications. Analysis of representative sequences (GenBank Accession Nos. HM585409 and HM585406) suggested that the isolated pathogen was C. karstii. Using published ITS data for C. karstii (3), a phylogenetic analysis was made via Bayesian inference, which shows that the isolated fungi belong to the C. karstii clade. Sequences of the isolates obtained in this study were deposited in GenBank (KC295235 and KC295236), and cultures were deposited in the Culture Collection of Phytopathogenic Fungi of the Universidade Federal Rural de Pernambuco (CMM, Recife, Brazil). Pathogenicity tests were conducted with the C. karstii strains on mango fruits cv. Tommy Atkins. Mycelial plugs taken from the margin of actively growing colonies (PDA) of each isolate were applied in shallow wounds (0.4 cm in diameter) at the medium region of the each fruit. PDA discs without fungal growing were used as controls. Inoculated fruits were placed in plastic containers lined with paper towels wetted in distilled water. The containers were partially sealed with plastic bags to maintain high humidity and incubated at 25°C in the dark. The plastic bags and paper towels were removed after 24 h, and fruits were kept at the same temperature. The experiment was arranged in a completely randomized design with four replicates per treatment (isolate) and four fruits per replicate. Typical anthracnose symptoms were observed after 10 days in mango fruits. C. karstii was successfully reisolated from symptomatic mango fruits to fulfill Koch's postulates. C. karstiiwas previously described from Orchidaceae in southwest China and the United States (2,3). To our knowledge, this is the first report of C. karstii causing mango anthracnose in Brazil and worldwide MenosFrom April to June 2010, mango fruits (Mangifera indica L.) (cv. Tommy Atkins) showing post-harvest anthracnose symptoms were collected during a survey conducted in São Francisco Valley, northeastern Brazil. Fruits affected by anthracnose showed sunken, prominent, dark brown to black decay spots. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g liter–1 streptomycin sulfate. Plates were incubated at 25°C in the dark for 5 to 7 days and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA (1). Identification was made using morphological characteristics and phylogenetic analysis. Two isolates (CMM 4101 and CMM 4102) presented colonies that had white aerial mycelia and orange conidial mass, varying between colorless and pale orange in reverse. Conidia were hyaline, cylindrical, and aseptate 14.52 (10.40 to 20.20) μm long and 4.90 (3.80 to 6.50) μm wide, length/width ratio = 3.0. Mycelial growth rate was 5.20 mm per day at 25°C. Morphological and cultural characterizations were consistent with the description of Colletotrichum karstii (3). PCR amplification by universal primers (ITS1/ITS4) and DNA sequencing of the internal transcribed spacer (ITS1-5.8S-ITS2 rRNA gene cluster) were conducted to confirm the identifications. Analysis of representative sequences (GenBank Accession Nos. HM58540... Mostrar Tudo |
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Palavras-Chave: |
Colletotrichum karstii. |
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Thesagro: |
Antracnose; Doença; Fungo; Manga; Mangifera Indica; Pós-Colheita. |
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Thesaurus Nal: |
Disease control; Mangoes; Postharvest technology; Postharvest treatment. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/91387/1/Angelica-2013-1.pdf
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Marc: |
LEADER 04089naa a2200325 a 4500
001 1969206
005 2023-06-06
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1094/PDIS-01-13-0002-PDN$2DOI
100 1 $aLIMA, N. B.
245 $aFirst report of mango anthracnose caused by Colletotrichum karstii in Brazil.$h[electronic resource]
260 $c2013
520 $aFrom April to June 2010, mango fruits (Mangifera indica L.) (cv. Tommy Atkins) showing post-harvest anthracnose symptoms were collected during a survey conducted in São Francisco Valley, northeastern Brazil. Fruits affected by anthracnose showed sunken, prominent, dark brown to black decay spots. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g liter–1 streptomycin sulfate. Plates were incubated at 25°C in the dark for 5 to 7 days and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA (1). Identification was made using morphological characteristics and phylogenetic analysis. Two isolates (CMM 4101 and CMM 4102) presented colonies that had white aerial mycelia and orange conidial mass, varying between colorless and pale orange in reverse. Conidia were hyaline, cylindrical, and aseptate 14.52 (10.40 to 20.20) μm long and 4.90 (3.80 to 6.50) μm wide, length/width ratio = 3.0. Mycelial growth rate was 5.20 mm per day at 25°C. Morphological and cultural characterizations were consistent with the description of Colletotrichum karstii (3). PCR amplification by universal primers (ITS1/ITS4) and DNA sequencing of the internal transcribed spacer (ITS1-5.8S-ITS2 rRNA gene cluster) were conducted to confirm the identifications. Analysis of representative sequences (GenBank Accession Nos. HM585409 and HM585406) suggested that the isolated pathogen was C. karstii. Using published ITS data for C. karstii (3), a phylogenetic analysis was made via Bayesian inference, which shows that the isolated fungi belong to the C. karstii clade. Sequences of the isolates obtained in this study were deposited in GenBank (KC295235 and KC295236), and cultures were deposited in the Culture Collection of Phytopathogenic Fungi of the Universidade Federal Rural de Pernambuco (CMM, Recife, Brazil). Pathogenicity tests were conducted with the C. karstii strains on mango fruits cv. Tommy Atkins. Mycelial plugs taken from the margin of actively growing colonies (PDA) of each isolate were applied in shallow wounds (0.4 cm in diameter) at the medium region of the each fruit. PDA discs without fungal growing were used as controls. Inoculated fruits were placed in plastic containers lined with paper towels wetted in distilled water. The containers were partially sealed with plastic bags to maintain high humidity and incubated at 25°C in the dark. The plastic bags and paper towels were removed after 24 h, and fruits were kept at the same temperature. The experiment was arranged in a completely randomized design with four replicates per treatment (isolate) and four fruits per replicate. Typical anthracnose symptoms were observed after 10 days in mango fruits. C. karstii was successfully reisolated from symptomatic mango fruits to fulfill Koch's postulates. C. karstiiwas previously described from Orchidaceae in southwest China and the United States (2,3). To our knowledge, this is the first report of C. karstii causing mango anthracnose in Brazil and worldwide
650 $aDisease control
650 $aMangoes
650 $aPostharvest technology
650 $aPostharvest treatment
650 $aAntracnose
650 $aDoença
650 $aFungo
650 $aManga
650 $aMangifera Indica
650 $aPós-Colheita
653 $aColletotrichum karstii
700 1 $aMARQUES, M. W.
700 1 $aMICHEREFF, S. J.
700 1 $aMORAIS JÚNIOR, M. A.
700 1 $aBARBOSA, M. A. G.
700 1 $aCÂMARA, M. P. S.
773 $tPlant Disease$gv. 97, n. 9, p. 1248, sept. 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 136 | |
| 101. |  | RODRIGUES, C. dos A.; FARIAS, J. W. de S.; BARBOSA, E. G. M.; BATISTA, D. da C.; BARBOSA, M. A. G. Crescimento de espécies de Campylocarpon em diferentes níveis de pH. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 11., 2016, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2016. p. 317-321. (Embrapa Semiárido. Documentos, 271).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 102. | | VILLELA, J. G. A.; RITSCHEL, P. S.; BARBOSA, M. A. G.; BACCIN, J. M. S.; ROSSATO, M.; MAIA, J. D. G.; FERREIRA, M. A. S. V. Detection of Xanthomonas citri pv. viticola on grapevine by real-time PCR and BIO-PCR using primers designed from pathogenicity and xanthomonadin gene sequences. European Journal of Plant Pathology, v. 155, n. 2, p. 445-459, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 103. | | SILVA, L. B. F. da; AMORIM, C. C. de; BARBOSA, J. da S.; BARBOSA, M. A. G.; BATISTA, D. da C. Efeito de fungicidas no crescimento micelial de Fusicoccum aesculis isolado de manga. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2012. p. 295-299. 1 CD-ROM. (Embrapa Semiárido. Documentos, 248).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 104. | | SILVA, L. B. F. da; AMORIM, C. C. de; BARBOSA, J. da S.; BARBOSA, M. A. G.; BATISTA, D. da C. Efeito de fungicidas no crescimento Micelial de Neofusicoccum parvum isolado de manga. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2012. p. 301-305. 1 CD-ROM. (Embrapa Semiárido. Documentos, 248).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 105. | | PONTES, S. S.; SILVA, F. M.; CORREIA, J. de S.; BARBOSA, J. da S.; BRAGA, M. B.; TERAO, D.; BARBOSA, M. A. G.; BATISTA, D. da C. Efeito de diferentes coberturas do solo na incidência de cancro das hastes em meloeiro. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2010. p. 136-140. (Embrapa Semiárido. Documentos, 228.).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 106. | | LOURENÇO, D. da S.; FARIAS, J. W. de S.; BARBOSA, E. G. M.; BATISTA, D. da C.; BARBOSA, M. A. G. Resistência de cultivares de videira ao cancro bacteriano. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 13., 2018, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2018. p. 391-396. (Embrapa Semiárido. Documentos, 283).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 107. | | LIMA, N. B.; BATISTA, M. V. de A.; MORAES JÚNIOR.; BARBOSA, M. A. G.; MICHEREFF, S, J.; HYDE, K. D.; CÂMARA, M. P. S. Five Colletotrichum species are responsible for mango anthracnose in Northeastern Brazil Fungal Diversity, v. 61, p. 75-88, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| 108. | | LIMA, N. B.; MARQUES, M. W.; MICHEREFF, S. J.; MORAIS JÚNIOR, M. A.; BARBOSA, M. A. G.; CÂMARA, M. P. S. First report of mango anthracnose caused by Colletotrichum karstii in Brazil. Plant Disease, v. 97, n. 9, p. 1248, sept. 2013.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Semiárido. |
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| 109. | | BARBOSA, J. da S.; AMORIM, C. C. de; FONSECA, C. L. da; LIMA NETO, F. P.; BARBOSA, M. A. G.; BATISTA, D. da C. Reação de genótipos de mangueira à infecção por Fusicoccum aesculis. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 6., 2011, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2011. p. 234-242. (Embrapa Semiárido. Documentos, 238).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 110. | | AMORIM, C. C. de; BARBOSA, J. da S.; FONSECA, C. L. da; LIMA NETO, F. P.; BARBOSA, M. A. G.; BATISTA, D. da C. Reação de genótipos de mangueira à infecção por Neofusicoccum parvum. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 6., 2011, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2011. p. 231-236. (Embrapa Semiárido. Documentos, 238).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 111. | | AMORIM, C. C. de; SILVA, F. M.; FREIRE, E. B.; SOUZA, W. C. O. de; PONTES, S. S.; BARBOSA, M. A. G.; TERAO, D.; BATISTA, D. da C. Relação entre incidência e severidade da ferrugem em videira cultivar Tempranillo. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2010. p. 154-159. (Embrapa Semiárido. Documentos, 228.)| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 113. | | FONSECA, C. L. da; NAUE, C. R.; OLIVEIRA, V. R. de; MELO, N. F. de; BATISTA, D. da C.; BARBOSA, M. A. G. Indexação de variedades de videira provenientes do cultivo in vitro visando a micropropagação de material livre do cancro-bacteriano. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 6., 2011, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2011. p. 59-65. (Embrapa Semiárido. Documentos, 238).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 114. | | SANTOS, L. O.; COSTA, V. S. de O.; FREIRE, E. B.; BATISTA, D. da C.; TERAO, D.; BARBOSA, M. A. G. Inibição in vitro de Xanthomonas campestris pv. viticola por indutores de resistência. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2010. p. 160-164. (Embrapa Semiárido. Documentos, 228.).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 115. | | LIMA, M. A. G.; PEIXOTO, A. R.; BORGES, I. V.; SILVA, M. S. e; BARBOSA, M. A. G.; CAVALCANTI, L. S. Induction of resistance to Xanthomonas campestris pv. viticola in grapevine plants. Revista Brasileira de Fruticultura, Jaboticabal, v. 39, n. 2, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| 116. | | ANDRADE, J. N. de; RODRIGUES, C. dos A.; FARIAS, J. W. de S.; BARBOSA, M. A. G.; BATISTA, D. da C. Infecções de Botryosphaeriaceae em gemas florais e ocorrência da podridão-peduncular em manga. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 11., 2016, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2016. p. 35-40. (Embrapa Semiárido. Documentos, 271).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 119. | | SILVA, F. M; ANJOS, F. G. J. dos; TERAO, D.; BARBOSA, M. A. G.; ANJOS, J. B. dos; BATISTA, D. da C. Padrão de dispersão de fungos em mangueira irrigada. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 4., 2009, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2009. p. 108-113. (Embrapa Semi-Árido. Documentos, 221).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 120. | | NAUE, C. R.; COSTA, V. S. O.; BARBOSA, M. A. G.; BATISTA, D. da C.; SOUZA, E. B.; MARIANO, R. L. R. Xanthomonas campestris pv. viticola on grapevine cutting tools and water: survival and disinfection. Journal of Plant Pathology, v. 96, n. 3, p. 451-458, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 136 | |
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