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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
22/05/2018 |
Data da última atualização: |
25/02/2019 |
Autoria: |
PRIORI, D.; VILLARREAL, D. Z.; CHANCÉ EK, V. M. de J.; LIMONES-BRIONES, V.; BARBIERI, R. L. |
Afiliação: |
Daniela Priori, CPACT; Daniel Zizumbo Villarreal, Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán A.C.; Víctor Manuel de Jesús Canché Ek, Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán A.C.; Verónica Limones-Briones, Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán A.C.; ROSA LIA BARBIERI, CPACT. |
Título: |
Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 3, p. 287-297, mar. 2018. |
Idioma: |
Inglês |
Notas: |
Título em português: Dinâmica evolutiva de Cucurbita argyosperma do centro mesoamericano de domesticação por meio de marcadores moleculares SSR. |
Conteúdo: |
The objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated that the evolutionary dynamics in the area occurred under five different ecological scenarios. Eighty-seven alleles with 75% to 100% polymorphic loci were identified. The greater genetic diversity in the weedy-domestic populations may have been the product of recombination due to the high gene flow between these populations promoted by pollinators and human selection. There is high gene flow between the wild and cultivated populations of C. argyrosperma in its domestication centre, highlighting the importance of conserving and maintaining these genetic resources. |
Palavras-Chave: |
Diversidade genética; Genetic diversity; Marcador microssatélite; Microsatellite markers; Recurso fitogenético. |
Thesagro: |
Abóbora; Cucurbitaceae. |
Thesaurus Nal: |
Plant genetic resources; Squashes. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177446/1/Evolutionary-dynamics-of-Cucurbita-argyrosperma.pdf
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Marc: |
LEADER 02388naa a2200289 a 4500 001 2091796 005 2019-02-25 008 2018 bl uuuu u00u1 u #d 100 1 $aPRIORI, D. 245 $aEvolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Dinâmica evolutiva de Cucurbita argyosperma do centro mesoamericano de domesticação por meio de marcadores moleculares SSR. 520 $aThe objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated that the evolutionary dynamics in the area occurred under five different ecological scenarios. Eighty-seven alleles with 75% to 100% polymorphic loci were identified. The greater genetic diversity in the weedy-domestic populations may have been the product of recombination due to the high gene flow between these populations promoted by pollinators and human selection. There is high gene flow between the wild and cultivated populations of C. argyrosperma in its domestication centre, highlighting the importance of conserving and maintaining these genetic resources. 650 $aPlant genetic resources 650 $aSquashes 650 $aAbóbora 650 $aCucurbitaceae 653 $aDiversidade genética 653 $aGenetic diversity 653 $aMarcador microssatélite 653 $aMicrosatellite markers 653 $aRecurso fitogenético 700 1 $aVILLARREAL, D. Z. 700 1 $aCHANCÉ EK, V. M. de J. 700 1 $aLIMONES-BRIONES, V. 700 1 $aBARBIERI, R. L. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 3, p. 287-297, mar. 2018.
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73. | | ROSSATO, M. BARBIERI, R. L.; SCHÄFER, A.; , ZACARIA, J. Caracterização molecular de populações de palmeiras do gênero butia do Rio Grande do Sul através de marcadores ISSR Magistra, Cruz das Almas, v. 19, n.4, p. 311-318, out./dez. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Embrapa Clima Temperado. |
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75. | | FERNANDES, R. C.; SOSINSKI JUNIOR, E. E.; BARBIERI, R. L. Caracterização de frutos de Butia odorata em área de conservação in situ no municipio de Tapes (RS). In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 27.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 20.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 4., 2018, Pelotas. Anais... Pelotas: UFPel, 2018.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Clima Temperado. |
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76. | | BARBIERI, R. L.; MEDEIROS, A. R. M. de. A cebola ao longo da história. In: BARBIERI, R.L. (Ed.). Cebola : ciência, arte e história. 2. ed.. Brasília, DF: Embrapa Informação Tecnológica; Pelotas: Embrapa Clima Temperado, 2007. p. 13-20Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
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