Data/hora: 23/06/2024 13:26:37

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 03/07/2017
Data da última atualização: | 03/07/2017
Autoria: | FANÉ, R.; KASSOGUÉ, A.; TRAORÉ, D.; DAO, S.; MAÏGA, K.; BAH, A.; VALICENTE, F. H.; BARBANA, A. H.
Afiliação: | FERNANDO HERCOS VALICENTE, CNPMS.
Título: | Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis.
Ano de publicação: | 2017
Fonte/Imprenta: | African Journal of Biotechnology, v. 16, n. 17, p. 983-988, 2017.
DOI: | 10.5897/AJB2017.15908
Idioma: | Inglês
Conteúdo: | The objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera?s larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest.
Thesagro: | Bacteria; Controle biológico.
Categoria do assunto: | S Ciências Biológicas
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/161312/1/Molecular-characterization-1.pdf
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (27798-1) - UPC - AP - DD -  -  -  - DIS