Data/hora: 13/06/2024 16:48:15

Biblioteca(s): | Embrapa Soja.
Data corrente: | 27/07/2009
Data da última atualização: | 04/08/2017
Autoria: | MENNA, P.; PEREIRA, A. A.; BANGEL, E. V.; HUNGRIA, M.
Afiliação: | PÂMELA MENNA, UEL/CNPq-MCT; ALAN ALVES PEREIRA, CNPq-MCT; ELIANE VILLAMIL BANGEL, FEPAGRO; MARIANGELA HUNGRIA DA CUNHA, CNPSO.
Título: | Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool.
Ano de publicação: | 2009
Fonte/Imprenta: | Symbiosis, Philadelphia, v. 48, n. 1-3, p. 120-130, Feb. 2009.
ISSN: | 0334-5114
DOI: | 10.1007/BF03179991
Idioma: | Inglês
Conteúdo: | With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%&#8758;80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies.
Thesagro: | Bactéria; Fixação de Nitrogênio; Inoculante; Taxonomia.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (29444-1) - UPC - AP - PP - 11637 -  - 11637 - DIS