Data/hora: 22/06/2024 15:03:02

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	28/10/2019	
Data da última atualização: 	15/09/2020	
Autoria: 	RIBEIRO, N. V. da S.; VIDAL, M. S.; BARRIOS, S. C. L.; BALDANI, J. I.	
Afiliação: 	NATALIA VIRGINIA DA SILVA RIBEIRO, UFRRJ; MARCIA SOARES VIDAL, CNPAB; SANZIO CARVALHO LIMA BARRIOS, CNPGC; JOSE IVO BALDANI, CNPAB.	
Título: 	Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Plant and Soil, First Online: 27 August 2019	
ISSN: 	1573-5036	
DOI: 	https://doi.org/10.1007/s11104-019-04263-y	
Idioma: 	Inglês	
Notas: 	Publicado na Plant and Soil, v. 451, p. 187–205, 2020.	
Conteúdo: 	Background and aims Diazotrophic bacteria, including those of the genus Azospirillum and Nitrospirillum, colonize Brachiaria genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from Brachiaria genotypes. Methods Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20 Brachiaria genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and nifH sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose. Results The diazotrophic population ranged from 102 to 108 g?1 rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera Azospirillum and Nitrospirillum, and revealed the presence of 14 other diazotrophic genera. The genus Nitrospirillum was detected colonizing all niches of most Brachiaria species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation. Conclusions The results showed a high diversity of diazotrophic bacterial species colonizing 20 Brachiaria genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizer	
Palavras-Chave: 	Diazotrophic bacteria; Gene Sequencing; Phylogenetic positioning.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (41237-1) - UPC - AP - DD -  -  -  - DIS