Data/hora: 16/06/2024 23:50:22

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo.
Data corrente: | 21/02/2022
Data da última atualização: | 05/09/2022
Autoria: | ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C.
Afiliação: | EDUARDO ANDRÉ ROESLER, UPF; NATALIA BALBINOTT, UFRGS; RENATA GABRIELA SCHROEDER, UPF; ELENE YAMAZAKI LAU, CNPT; MARIA IMACULADA PONTES M LIMA, CNPT; SANDRA PATUSSI BRAMMER, CNPT; FRANCISCO JOSE LIMA ARAGAO, Cenargen; JOSE MAURICIO CUNHA FERNANDES, CNPT; CAROLINA CARDOSO DEUNER, UPF.
Título: | An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.
Ano de publicação: | 2022
Fonte/Imprenta: | Tropical Plant Pathology, 16 Feb. 2022
DOI: | https://doi.org/10.1007/s40858-022-00497-x
Idioma: | Inglês
Conteúdo: | Abstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis.
Palavras-Chave: | Inoculation assay; Protocol.
Thesagro: | Doença Fúngica; Ferrugem; Fusarium Graminearum; Trigo.
Thesaurus Nal: | Arabidopsis thaliana; Fusarium head blight.
Categoria do assunto: | --
Registro original: | Embrapa Trigo (CNPT)

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