Data/hora: 13/06/2024 22:26:45

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	07/02/2022	
Data da última atualização: 	07/02/2022	
Autoria: 	TAIRA, A. R.; BATISTA, R. I. T. P.; SANTOS, J. D. R.; PINTO, P. H. N.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; SOUZA-FABJAN, J. M. G.; UNGERFELD, R.; FONSECA, J. F. da; BRANDÃO, F. Z.	
Afiliação: 	AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense - Niterói, RJ, Brazil; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense - Niterói, RJ, Brazil; JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense - Niterói, RJ, Brazil; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; MARIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense - Niterói, RJ, Brazil; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense - Niterói, RJ, Brazil; VIVIANE LOPES BRAIR, Universidade Federal Fluminense - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense - Niterói, RJ, Brazil.	
Título: 	Progestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Animal Reproduction Science, v. 238, 106938, Mar. 2022.	
DOI: 	https://doi.org/10.1016/j.anireprosci.2022.106938	
Idioma: 	Inglês	
Conteúdo: 	Abstract: This study aimed to compare the effect of the administration of either medroxyprogesterone acetate (MPA) or progesterone (P4) in superovulation (SOV) treatments applied during the first follicular wave on follicular development, embryo yield, and the expression of genes related to pluripotency maintenance, differentiation of the trophectoderm, cell growth and differentiation, apoptosis and energy metabolism in sheep embryos. The estrous cycle of 36 multiparous ewes was synchronized with a short protocol, and the animals were randomly allocated to three groups. At the beginning of SOV, 12 ewes per treatment received an intravaginal sponge impregnated with 60 mg of MPA (TMPA), or an intravaginal device containing 0.33 g of P4 (TP4), or received no progestogen treatment (CON). The device was kept until the fifth dose of FSH. Ewes were mated with five fertile rams. Gene expression was performed by RT-qPCR using grade I and II blastocysts. The numbers of corpora lutea, total structures and viable embryos recovered per ewe were similar (P > 0.05) among groups. However, the viability rate was higher in TP4 (71.9 ± 16.3%) compared to CON (24.4 ± 16.8%; P = 0.01) and similar to TMPA (49.9 ± 16.3%; P = 0.2). Similarly, when compared with CON, treatment with P4 or MPA positively regulated the TGFB1 transcript involved in cell proliferation and differentiation (P = 0.01 and P = 0.03, respectively). In conclusion, supplementation with P4 during the first follicular wave of the estrous cycle improves embryo viability and alters the expression of the TGFB1 gene.	
Palavras-Chave: 	Day 0 protocol; Embryo production; Multiple ovulations; Progestins.	
Thesaurus Nal: 	Animal reproduction; Estrous cycle; Ewes; Follicular development; Gene expression; Gene expression regulation; Medroxyprogesterone; Ovarian development; Progesterone; Small ruminants; Superovulation; Synthetic progestogens.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (39976-1) - UPC - AP - DD -  -  -  - DIS