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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
11/03/2005 |
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Data da última atualização: |
13/11/2019 |
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Autoria: |
BRONOMELLI, A.; MERCIER, L.; FRANCHEL, J.; BAILLIEUL, F.; BENIZRI, E.; MAURO, M.-C. |
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Título: |
Response of grapevine defenses to UV-C exposure. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
American Journal of Enology and Viticulture, Davis, v. 55, n. 1, p. 51-59, 2004. |
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Páginas: |
p. 51-59 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The defense potential of a tolerant American Yitis rupestris cultivar (Rupestris du Lot) and a susceptible European Vitis vinifera cultivar (Chardonnay) in response to UV-C irradiation was investigated, The expression of eight defense-related genes coding for enzymes of the phenylpropanoid pathway (phenylalanine ammonia Jyase and stilbene synthase), the octadecanoid pathway (lipoxygenase), and pathogenesis-related proteins (class I and Ill chitinases, B-I ,3-glllcanase, cIass 6 pathogenesis-related protein, and class 10 pathogenesis-related protein) was followed by real-time reverse transcription polymerase chain reaction (RT-PCR). Phenolic compound accumulation was monitored by microscopic observation. Accumulation of resveratrol, a major grapevine phytoalexin, was evaluated by HPLC, and chitinase and fi-I ,3- glucanase enzyme activities were measured. Both grapevine species responded to UV-C treatment by enhancement of defense mechanisms. Intensity of some defense responses was correlated with tolerance to diseases, as previously described for stilbene accumulation: the tolerant species responded more intensely to UV-C exposure than the susceptibJe one. UV-C irradiation is a practical and reproducible method for inducing grapevine defense responses and can be useful in determining the defense potentiaJ of grapevine cultivars. Keys words: grapevine, UV-C (200 to 280 nm), defense mechanisms, real-time RT-PCR Abbreviations: class I chitinase (Chitlb); class III chitinase (CH3); Iipoxygenase (LOX); phenylalanine ammonia lyase (PAL); class 6 pathogenesis-related protein (PR-6); class 10 pathogenesis-reJated protein (PR-IO); stilbene synthase (StSy) MenosAbstract: The defense potential of a tolerant American Yitis rupestris cultivar (Rupestris du Lot) and a susceptible European Vitis vinifera cultivar (Chardonnay) in response to UV-C irradiation was investigated, The expression of eight defense-related genes coding for enzymes of the phenylpropanoid pathway (phenylalanine ammonia Jyase and stilbene synthase), the octadecanoid pathway (lipoxygenase), and pathogenesis-related proteins (class I and Ill chitinases, B-I ,3-glllcanase, cIass 6 pathogenesis-related protein, and class 10 pathogenesis-related protein) was followed by real-time reverse transcription polymerase chain reaction (RT-PCR). Phenolic compound accumulation was monitored by microscopic observation. Accumulation of resveratrol, a major grapevine phytoalexin, was evaluated by HPLC, and chitinase and fi-I ,3- glucanase enzyme activities were measured. Both grapevine species responded to UV-C treatment by enhancement of defense mechanisms. Intensity of some defense responses was correlated with tolerance to diseases, as previously described for stilbene accumulation: the tolerant species responded more intensely to UV-C exposure than the susceptibJe one. UV-C irradiation is a practical and reproducible method for inducing grapevine defense responses and can be useful in determining the defense potentiaJ of grapevine cultivars. Keys words: grapevine, UV-C (200 to 280 nm), defense mechanisms, real-time RT-PCR Abbreviations: class I chitinase (Chitlb); class III chit... Mostrar Tudo |
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Palavras-Chave: |
Mecanismo de defesa; Radiação UV-C. |
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Thesagro: |
Uva; Variedade; Viticultura. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02370naa a2200253 a 4500
001 1540265
005 2019-11-13
008 2004 bl uuuu u00u1 u #d
100 1 $aBRONOMELLI, A.
245 $aResponse of grapevine defenses to UV-C exposure.$h[electronic resource]
260 $c2004
300 $ap. 51-59
520 $aAbstract: The defense potential of a tolerant American Yitis rupestris cultivar (Rupestris du Lot) and a susceptible European Vitis vinifera cultivar (Chardonnay) in response to UV-C irradiation was investigated, The expression of eight defense-related genes coding for enzymes of the phenylpropanoid pathway (phenylalanine ammonia Jyase and stilbene synthase), the octadecanoid pathway (lipoxygenase), and pathogenesis-related proteins (class I and Ill chitinases, B-I ,3-glllcanase, cIass 6 pathogenesis-related protein, and class 10 pathogenesis-related protein) was followed by real-time reverse transcription polymerase chain reaction (RT-PCR). Phenolic compound accumulation was monitored by microscopic observation. Accumulation of resveratrol, a major grapevine phytoalexin, was evaluated by HPLC, and chitinase and fi-I ,3- glucanase enzyme activities were measured. Both grapevine species responded to UV-C treatment by enhancement of defense mechanisms. Intensity of some defense responses was correlated with tolerance to diseases, as previously described for stilbene accumulation: the tolerant species responded more intensely to UV-C exposure than the susceptibJe one. UV-C irradiation is a practical and reproducible method for inducing grapevine defense responses and can be useful in determining the defense potentiaJ of grapevine cultivars. Keys words: grapevine, UV-C (200 to 280 nm), defense mechanisms, real-time RT-PCR Abbreviations: class I chitinase (Chitlb); class III chitinase (CH3); Iipoxygenase (LOX); phenylalanine ammonia lyase (PAL); class 6 pathogenesis-related protein (PR-6); class 10 pathogenesis-reJated protein (PR-IO); stilbene synthase (StSy)
650 $aUva
650 $aVariedade
650 $aViticultura
653 $aMecanismo de defesa
653 $aRadiação UV-C
700 1 $aMERCIER, L.
700 1 $aFRANCHEL, J.
700 1 $aBAILLIEUL, F.
700 1 $aBENIZRI, E.
700 1 $aMAURO, M.-C.
773 $tAmerican Journal of Enology and Viticulture, Davis$gv. 55, n. 1, p. 51-59, 2004.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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